Combinatorial Peptide Library for Probing the Selectivity of the s-1 Subsite of Proteases

A combinatorial tetrapeptide library, Suc-Ala-Phe-Arg-AA₁-OR, in which R = p-formamidobenzyl ester and AA₁ = 17 of the 20 natural occurring amino acids, has been synthesized chemically and separated by a reverse phase HPLC. The library was used to study the s-1 subsite specificity of various proteases. The preferred substrate at the s-1 subsite of chymotrypsin is in the order of Trp > Tyr > Phe > Met > Leu. This agreed with the reported data that the favored substrate at the s-1 subsite for chymotrypsin-catalyzed hydrolysis is an aromatic amino acid residue. The hydrophobic amino acid residues at this subsite can be hydrolysized after a longer incubating time. This procedure of selective hydrolysis of a peptide library was used to probe the selectivity of s-1 subsites of four proteases isolated from Bacillus stearothermophilus, subtilisin Carlsberg, subtilisin BPN' and an engineered protease subtilisin 8397. The protease from Bacillus stearothermophilus favored the substrate with residue Lys, and Arg at the s-1 subsite as a trypsin-like protease. The relative reactivities of amino acid residues in the protease-catalyzed hydrolysis of the library can be used as a fingerprint to identify the protease in a protease family.     

Synthesis of Pyrrolidine Derivatives by the Palladium-Catalyzed Cyclization of N-(2-Cyclohexenyl)bromoacetamide Derivatives

N-(2-Cyclohexenyl)bromoacetamide derivatives underwent intramolecular cyclization to give pyrrolidine derivatives in the presence of a base and a catalytic amount of palladium catalyst.     

Parallel Synchronous and Asynchronous Space-Decomposition Algorithms for Large-Scale Minimization Problems

Three parallel space-decomposition minimization (PSDM) algorithms, based on the parallel variable transformation (PVT) and the parallel gradient distribution (PGD) algorithms (O.L. Mangasarian, SIMA Journal on Control and Optimization, vol. 33, no. 6, pp. 1916–1925.), are presented for solving convex or nonconvex unconstrained minimization problems. The PSDM algorithms decompose the variable space into subspaces and distribute these decomposed subproblems among parallel processors. It is shown that if all decomposed subproblems are uncoupled of each other, they can be solved independently. Otherwise, the parallel algorithms presented in this paper can be used. Numerical experiments show that these parallel algorithms can save processor time, particularly for medium and large-scale problems. Up to six parallel processors are connected by Ethernet networks to solve four large-scale minimization problems. The results are compared with those obtained by using sequential algorithms run on a single processor. An application of the PSDM algorithms to the training of multilayer Adaptive Linear Neurons (Madaline) and a new parallel architecture for such parallel training are also presented.     

Optimal production run length for products sold with warranty

This article studies the optimal production run length for a deteriorating production system in which the products are sold with free minimal repair warranty. The deterioration process of the system is characterized by a two-state continuous-time Markov chain. For products sold with free minimal repair warranty, we show that there exists a unique optimal production run length such that the expected total cost per item is minimized. Since there is no closed form expression for the optimal production run length, an approximate solution is derived. In addition, three special cases which provide bounds for searching the optimal production run length are investigated and some sensitivity analysis is carried out to study the effects of the model parameters on the optimal production run length. Finally, a numerical example is given to evaluate the performance of the optimal production run length.     

Folding and Unfolding of Exogenous G-Rich Oligonucleotides in Live Cells by Fluorescence Lifetime Imaging Microscopy of o-BMVC Fluorescent Probe

Guanine-rich oligonucleotides (GROs) can self-associate to form G-quadruplex (G4) structures that have been extensively studied in vitro. To translate the G4 study from in vitro to in live cells, here fluorescence lifetime imaging microscopy (FLIM) of an o-BMVC fluorescent probe is applied to detect G4 structures and to study G4 dynamics in CL1-0 live cells. FLIM images of exogenous GROs show that the exogenous parallel G4 structures that are characterized by the o-BMVC decay times (≥2.4 ns) are detected in the lysosomes of live cells in large quantities, but the exogenous nonparallel G4 structures are hardly detected in the cytoplasm of live cells. In addition, similar results are also observed for the incubation of their single-stranded GROs. In the study of G4 formation by ssHT23 and hairpin WT22, the analyzed binary image can be used to detect very small increases in the number of o-BMVC foci (decay time ≥ 2.4 ns) in the cytoplasm of live cells. However, exogenous ssCMA can form parallel G4 structures that are able to be detected in the lysosomes of live CL1-0 cells in large quantities. Moreover, the photon counts of the o-BMVC signals (decay time ≥ 2.4 ns) that are measured in the FLIM images are used to reveal the transition of the G4 formation of ssCMA and to estimate the unfolding rate of CMA G4s with the addition of anti-CMA into live cells for the first time. Hence, FLIM images of o-BMVC fluorescence hold great promise for the study of G4 dynamics in live cells.     

THE SPECTROSCOPY OF FOUR-BARYON SYSTEM

In this paper, using SU(6) symmetry theory four-baryon system is discussed and a method of analyzing experimental spectra is provided. Because of the gound states of _ΛΛ⁴H, _ΛΛ⁴He_{, Λ}⁴He and ⁴He are in the same I. R. of SU(6), on can derives masses for _ΛΛ⁴H, _ΛΛ⁴He from the experimental data of _Λ⁴H, _Λ⁴He, and ⁴He. Calculation shows that these J^π=0⁺ double Λ hypernuclei _ΛΛ⁴H, _ΛΛ⁴He are not likely bound states.     

Random RotBoost: An Ensemble Classification Method Based on Rotation Forest and AdaBoost in Random Subsets and Its Application to Clinical Decision Support

In the era of bathing in big data, it is common to see enormous amounts of data generated daily. As for the medical industry, not only could we collect a large amount of data, but also see each data set with a great number of features. When the number of features is ramping up, a common dilemma is adding computational cost during inferring. To address this concern, the data rotational method by PCA in tree-based methods shows a path. This work tries to enhance this path by proposing an ensemble classification method with an AdaBoost mechanism in random, automatically generating rotation subsets termed Random RotBoost. The random rotation process has replaced the manual pre-defined number of subset features (free pre-defined process). Therefore, with the ensemble of the multiple AdaBoost-based classifier, overfitting problems can be avoided, thus reinforcing the robustness. In our experiments with real-world medical data sets, Random RotBoost reaches better classification performance when compared with existing methods. Thus, with the help from our proposed method, the quality of clinical decisions can potentially be enhanced and supported in medical tasks.     

Exploration of an Efficient Method for Optical Resolution of Etodolac

The exploration of an efficient method for resolving etodolac using either L‐cinchonidinium salt or chiral isopinocamphenol diastereomeric esters is described herein. Furthermore, racemization mechanism of chiral etodolac is rationalized in terms of an isotope labeling experiment.     

New organo‐soluble aromatic polyimides based on 3,3′,5,5′‐tetrabromo‐2,2‐bis[4‐(3,4‐dicarboxyphenoxy)phenyl]propane dianhydride and aromatic diamines

New aromatic tetracarboxylic dianhydride, having isopropylidene and bromo‐substituted arylene ether structure 3,3′,5,5′‐tetrabromo‐2,2‐bis[4‐(3,4‐dicarboxyphenoxy)phenyl]propane dianhydride, was synthesized by the reaction of 4‐nitrophthalonitrile with 3,3′,5,5′‐tetrabromobisphenol A, followed by alkaline hydrolysis of the intermediate bis(ether dinitrile) and subsequent dehydration of the resulting bis(ether diacid). The novel aromatic polyetherimides having inherent viscosities up to 1.04 dL g⁻¹ were obtained by either a one‐step or a conventional two‐step polymerization process starting from the bis(ether anhydride) and various aromatic diamines. All the polyimides showed typical amorphous diffraction patterns. Most of the polyimides were readily soluble in common organic solvents such as N,N‐dimethylacetamide (DMAc), N‐methyl‐2‐pyrrolidone (NMP), pyridine, and even in less polar solvents like chloroform and tetrahydrofuran (THF). These aromatic polyimides had glass transition temperatures in the range of 256–303°C, depending on the nature of the diamine moiety. Thermogravimetric analysis (TGA) showed that all polymers were stable, with 10% weight loss recorded above 470°C in nitrogen. © 1999 John Wiley & Sons, Inc. J Polym Sci A: Polym Chem 37: 1673–1680, 1999     

Computation of three‐dimensional flow around square and circular piers

The aim of the present study is to investigate, by numerical simulation, the three‐dimensional turbulent flow field around square and circular piers. The numerical model employs a finite volume method based on MacCormack's explicit predictor–corrector scheme to solve weakly compressible hydrodynamic equations for turbulent flow. Computed results are compared with Dargahi's experimental measurements to assess the validity of the proposed model. Very good agreements are obtained. The results of flow simulation indicate that near the upstream face of the pier there exists a downflow, which joins the separated flow to form the horseshoe vortex stretched around the pier. This horseshoe vortex interacts with the wake vortex to create the upflow behind the pier. These phenomena appear to be very important to the mechanism of scouring around the pier. In general, the flow patterns for the square and circular piers are similar. However, the strengths of the downflow and horseshoe vortex are greater in the case of the square pier. The position of the horseshoe vortex around the circular pier is closer to the front face than that around the square pier. In the meantime, the domain of the wake flow in the case of the square pier is greater than that in the case of the circular one. Copyright © 2000 John Wiley & Sons, Ltd.