A series of o-tropyliobiphenyl tetradfluoroborates has been synthesized. Evidences are presented to show that exhibit strong intramolecular charge-transfer interaction between tropylium ion and remote aryl ring. The structure of was determined by single crystal X-ray diffraction study. 相似文献
A capillary electrophoresis (CE)-based method for the in vitro detection and monitoring of nucleotide-triphosphatase activity
is described. This robust and reproducible method was used to investigate GTPase activity of a recombinant protein construct
containing the catalytic domain of Human SEPT4/Bradeion β (GST-rDGTPase). This example application demonstrates that the CE
technique can replace classical radioactive methods for GTPase activity assays and may be used as a routine analytical tool.
Enzyme kinetics of GST-rDGTPase was studied and yielded the following kinetic parameters: vmax = 1.7 μM min−1 ± 0.1, Km = 1.0 mM ± 0.3, and apKcat = 9 × 10−3 s−1. In addition the effect of co-factors such as Mg2+ and Mn2+ on the catalytic activity was investigated. The described analytical method was also shown to be useful to analyze diphosphated
and triphosphated forms of other nucleotides. 相似文献
The cloning and functional expression of Mycobacterium tuberculosis Rv3377c in Escherichia coli revealed that this gene encodes the diterpene cyclase for producing (+)-5(6),13-halimadiene-15-ol, which accepts geranylgeranyldiphosphate as the intrinsic substrate. 相似文献
A one-dimensional coordination polymer of [Mn(II)(hfac)2] bridged by new bowl-like ligands of cavitands was prepared and the crystal structure was determined by X-ray crystal analysis. Ethyl acetate molecules are necessarily included as guest molecules in each of the void-space cavities of the cavitands, and then are held by weak interaction forces. 相似文献
Non-noble metal-based hydrogenation catalysts have limited practical applications because they exhibit low activity, require harsh reaction conditions, and are unstable in air. To overcome these limitations, herein we propose the alloying of non-noble metal nanoparticles with phosphorus as a promising strategy for developing smart catalysts that exhibit both excellent activity and air stability. We synthesized a novel nickel phosphide nanoalloy (nano-Ni2P) with coordinatively unsaturated Ni active sites. Unlike conventional air-unstable non-noble metal catalysts, nano-Ni2P retained its metallic nature in air, and exhibited a high activity for the hydrogenation of various substrates with polar functional groups, such as aldehydes, ketones, nitriles, and nitroarenes to the desired products in excellent yields in water. Furthermore, the used nano-Ni2P catalyst was easy to handle in air and could be reused without pretreatment, providing a simple and clean catalyst system for general hydrogenation reactions. 相似文献