全文获取类型
收费全文 | 17592篇 |
免费 | 2719篇 |
国内免费 | 2287篇 |
专业分类
化学 | 13590篇 |
晶体学 | 277篇 |
力学 | 942篇 |
综合类 | 187篇 |
数学 | 1721篇 |
物理学 | 5881篇 |
出版年
2024年 | 28篇 |
2023年 | 260篇 |
2022年 | 457篇 |
2021年 | 543篇 |
2020年 | 618篇 |
2019年 | 633篇 |
2018年 | 494篇 |
2017年 | 509篇 |
2016年 | 798篇 |
2015年 | 776篇 |
2014年 | 947篇 |
2013年 | 1308篇 |
2012年 | 1558篇 |
2011年 | 1643篇 |
2010年 | 1177篇 |
2009年 | 1025篇 |
2008年 | 1170篇 |
2007年 | 990篇 |
2006年 | 990篇 |
2005年 | 934篇 |
2004年 | 743篇 |
2003年 | 653篇 |
2002年 | 721篇 |
2001年 | 480篇 |
2000年 | 436篇 |
1999年 | 385篇 |
1998年 | 285篇 |
1997年 | 262篇 |
1996年 | 253篇 |
1995年 | 236篇 |
1994年 | 188篇 |
1993年 | 175篇 |
1992年 | 161篇 |
1991年 | 138篇 |
1990年 | 123篇 |
1989年 | 99篇 |
1988年 | 86篇 |
1987年 | 57篇 |
1986年 | 61篇 |
1985年 | 63篇 |
1984年 | 33篇 |
1983年 | 29篇 |
1982年 | 21篇 |
1981年 | 20篇 |
1980年 | 9篇 |
1979年 | 7篇 |
1974年 | 2篇 |
1971年 | 2篇 |
1957年 | 6篇 |
1936年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
221.
An end-channel amperometric detector with a guide tube for working electrode was designed and integrated on a home-made glass microchip. The guide tube was directly patterned and fabricated at the end of the detection reservoir, which made the fixation and alignment of working electrode relatively easy. The fabrication was carried out in a two-step etching process. A 30 μm carbon fiber microdisk electrode and Pt cathode were also integrated onto the amperometric detector. The baseline separation of dopamine (DA), catechol (CA) and epinephrine (EP) was achieved within 80 s. Relative standard deviations of not more than 5.2% were obtained for both peak currents and migration times of DA and CA (n=5). Using standard adding method, DA in tLrine and plasma samples was detected. The recoveries were in the range of 83%—103%. 相似文献
222.
Native non‐covalently bonded protein‐protein and protein‐substrate complexes are of great interest and have been extensively studied by electrospray ionization mass spectrometry (ESI‐MS). Multiply charged protein homomultimeric complexes are shown to form by ESI‐MS. This study addresses factors that can artificially induce the formation of multiply charged protein homomultimeric complexes. Cytochrome c (Cyt c) and ubiquitin, which are monomers in solution, were found to generate (Cyt c)mn+ by electrospray ionization (ESI). The homomultimeric complexes were not limited to dimeric complexes but include also multiply charged trimers, tetramers, and pentamers. The observation of these homomultimeric complexes has never been revealed from a Cyt c solution at the concentration as low as 10 μM. Increasing the concentration of Cyt c enhanced the formation of (Cyt c)mn+ as expected; however, the protein concentration does not affect the relative intensities of monomeric and dimeric complexes. Additionally the enrichment of NH4OH also promotes the formation of (Cyt c)mn+. Notably, source collision‐induced dissociations (source‐CID) of (Cyt c)mn+ alter the charge state distribution (CSD) and may lead to an incorrect interpretation of Cyt c conformations. Hence, extra care should be taken when using CSD to interpret the conformation of a protein derived from ESI‐MS. 相似文献
223.
高效石墨浮选剂的合成金婵,薛玉,汪进,刘欣荣,秦英海(哈尔滨工业大学应用化学系哈尔滨150001)(大庆石化总公司大庆)关键词石墨,浮选剂,合成,混合烃由于石墨具有良好的力学性能、导电、高熔点等性质,广泛用于原子能、宇航和民用工业。隐晶质石墨属于微晶... 相似文献
224.
Thermomagnetic experiments are shown to be a useful and rapid technique for studying phase changes involving magnetic materials. Particular examples are chosen from Chromindur II (Fe28Cr10.5Co) alloys having different thermal histories. The Curie temperature of the single phase alloy is found to be ~650°C and the metastable spinodal decomposition boundary is at essentially the same temperature. The Cr-rich near equilibrium decomposition product is less magnetic and has a lower Tc, while the Cr-deficient product is more magnetic, with a Tc ? 720°C.Comparisons are made at different heating and cooling rates between temperatures measured using magnetic standards and those measured by a thermocouple in close proximity to the sample. 相似文献
225.
Four lanthanide coordination polymers with benzophenone‐4,4′‐dicarboxylic acid (H2bpndc) and 1,10‐phenanthroline (phen), [Ln2(bpndc)3(phen)] (Ln=La (1), Pr (2) and Tb (3)), [Yb(bpndc)15(phen)].05H2O (4) were obtained through solvothermal synthesis. The crystallographic data show that 1, 2, and 3 are isostructural, the Ln(III) ions in 1, 2 and 3 are all eight‐ and ten‐coordinated, respectively, and thus the Ln(III) ions are connected by bpndc ligands, resulting in an interpenetrating 3D structure. While in 4, the Yb(III) ions are eight‐coordinated and connected by bpndc ligands into a 3D structure with 1D rhombic channels, which result from the effect of lanthanide contraction from La(III) to Yb(III) ions, and the bpndc ligands in 1, 2, 3, and 4 display three types of coordination modes. 相似文献
226.
227.
A fast and matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS) compatible protein staining method in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1- and 2-D SDS-PAGE) is described. It is based on the counterion dye staining method that employs oppositely charged two dyes, zincon (ZC) and ethyl violet (EV) to form an ion-pair complex. The protocol, including fixing, staining and quick washing steps, can be completed in 1-1.5 h depending upon gel thickness. It has a sensitivity of 4-8 ng, comparable to that of colloidal Coomassie Brilliant Blue G (CBBG) staining with phosphoric acid in the staining solution. The counterion dye stain does not induce protein modifications that complicate interpretation of peptide mapping data from MS. Considering the speed, sensitivity and compatibility with MS, the counterion dye stain may be more practical than any other dye-based protein stains for routine proteomic researches. 相似文献
228.
229.
Aqua dissociation nature of cesium hydroxide 总被引:1,自引:0,他引:1
To understand the mechanism of aqueous base dissociation chemistry, the ionic dissociation of cesium-hydroxide in water clusters is examined using density functional theory and ab initio calculations. In this study, we report hydrated structures, stabilities, thermodynamic quantities, dissociation energies, infrared spectra, and electronic properties of CsOH(H(2)O)(n=0-4). With the addition of water molecules, the Cs-OH bond lengthened significantly from 2.46 A for n=1 to 3.08 A for n=4, which causes redshift in Cs-O stretching frequency. It is found that three water molecules are needed for the dissociation of Cs-OH, in contrast to the case of strong acid dissociation which requires at least four water molecules. However, the dissociation for n=3 could be considered as incomplete because a very weak CS em leader OH stretch mode is still present, while that for n=4 is complete since the Cs em leader OH mode no longer exists. This study can be related with hydration chemistry of cations and anions, and extended into the intra- and intercharge-transfer phenomena. 相似文献
230.
A background-free, fast protein staining method in polyacrylamide gel electrophoresis using an acidic dye, zincon (ZC) and a basic dye, ethyl violet (EV) is described. It is based on the counterion dye staining technique that employs two oppositely charged dyes to form an ion-pair complex in staining solution. The selective binding of free dye molecules to proteins in acidic solution produces bluish violet-colored bands. It is a rapid and end-point staining procedure, involving only fixing and staining steps that are completed in 1-1.5 h. The detection limit of this method is 8-15 ng of protein that is comparable to the sensitivity of the colloidal Coomassie Brilliant Blue G (CBBG) stain. Due to its sensitivity and speed, this stain may be more practical than any other dye-based stains for routine laboratory purposes. 相似文献