排序方式: 共有43条查询结果,搜索用时 15 毫秒
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Marijke WA de Backer Carlos P Fitzsimons Maike AD Brans Mieneke CM Luijendijk Keith M Garner Erno Vreugdenhil Roger AH Adan 《BMC neuroscience》2010,11(1):81
Background
This study compared the transduction efficiencies of an adeno-associated viral (AAV) vector, which was pseudotyped with an AAV1 capsid and encoded the green fluorescent protein (GFP), with a lentiviral (LV) vector, which was pseudotyped with a VSV-G envelop and encoded the discosoma red fluorescent protein (dsRed), to investigate which viral vector transduced the lateral hypothalamus or the amygdala more efficiently. The LV-dsRed and AAV1-GFP vector were mixed and injected into the lateral hypothalamus or into the amygdala of adult rats. The titers that were injected were 1 × 108 or 1 × 109 genomic copies of AAV1-GFP and 1 × 105 transducing units of LV-dsRed. 相似文献24.
Cooper DB Read RW Timperley CM Williams NH Black RM 《Journal of chromatography. A》2004,1040(1):83-95
Organophosphorus nerve agents and their precursors, specifically listed in the schedules of chemicals in the Annex to the Chemical Weapons Convention (CWC), include analogues with C1-C3 alkyl groups on phosphorus. The Organisation for the Prohibition of Chemical Weapons (OPCW) requires designated laboratories to unequivocally identify isomeric propyl groups bonded to phosphorus in analytes that may be present in samples submitted for analysis. Homologous series of isomeric pairs of dialkyl iso- and n-propylphosphonates, alkyl iso- and n-propylphosphonochloridates, and alkyl iso- and n-propylphosphonofluoridates, have been analysed by liquid chromatography-ion trap tandem mass spectrometry and/or by gas chromatography-Fourier transform infrared spectroscopy. The results show that P-propyl isomers can be reliably differentiated by collision induced dissociation (CID) of selected fragment ions and by their infrared P=O stretching and C-H deformation frequencies. 相似文献
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AM Cardoso SM Alexandre CM Barros AJ Correia NM Nibbering 《Rapid communications in mass spectrometry : RCM》1999,13(19):1885-1888
The collision-induced dissociation (CID) of deprotonated arylalkylamines of general formula R(1)C(6)H(4)CHR(2)CH(2)NR(3)(2) (where R(1) = H, OH, F or NO(2); R(2) = H or OH; R(3) = H or CH(3)) generated by negative chemical ionization with H(2)O and D(2)O as ionizing reagents, is discussed. The negative chemical ionization mass spectra show that, in the absence of a hydroxy group in the aromatic ring, deprotonation takes place at the benzylic position whereas the proton is lost from the OH group when present. The nitro compound forms only M(-.) ions. The CID spectra of the deprotonated molecules show that fragmentations are strongly dependent on the structural features of the molecules, namely the presence or absence of substituents in the aromatic ring or aliphatic chain. Copyright 1999 John Wiley & Sons, Ltd. 相似文献
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AM Johnston CM Scrimgeour MO Henry LL Handley 《Rapid communications in mass spectrometry : RCM》1999,13(14):1531-1534
The conversion of nitrate (NO(3)(-)) to 1-phenylazo-2-naphthol (Sudan-1) has been examined as a method for natural abundance measurement of delta(15)N of NO(3)(-). The reaction results in dilution of NO(3)(-)-N with only one reagent-derived N and the product is readily concentrated from dilute samples by reverse phase chromatography. There is systematic isotopic fractionation during the reaction, but this can be allowed for by analysing known NO(3)(-) standards along with each sample set. Sudan-1 prepared from surface water samples containing approximately 50 &mgr;g NO(3)(-)-N can be analysed by automated continuous flow isotope ratio mass spectrometry with a precision of 0.2 per thousand (one standard deviation) and the accuracy is not affected by interference from other nitrogenous species in the sample or reagents. Copyright 1999 John Wiley & Sons, Ltd. 相似文献
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Christopher M. Timperley R. Eric Banks Ian M. Young Robert N. Haszeldine 《Journal of fluorine chemistry》2011,132(8):541-547
Fluoroheterocyclic aldoximes were screened as therapeutic agents for the treatment of anticholinesterase poisoning. 2-Fluoropyridine-3- and -6-aldoxime, and 3-fluoropyridine-2- and -4-aldoxime, were synthesised. Attempts to obtain 3,5,6-trifluoropyridine-2,4-bis(aldoxime) and -2-aldoxime, however, proved unsuccessful. Pentafluorobenzaldoxime was prepared by oximation of pentafluorobenzaldehyde. Acid dissociation constants (pKa) and second-order rate constants (kox−) of the fluorinated pyridinealdoximes towards sarin were measured. 2,3,5,6-Tetrafluoropyridine-4-aldoxime had the best profile: its kox− approached that of the therapeutic oxime P2S (310 vs. 120 l mol−1 min−1), but its higher pKa (9.1 vs. 7.8) fell short of the target figure of 8 required for reactivation of inhibited acetylcholinesterase in vivo. N-alkylation of the fluorinated pyridine-aldoximes may reduce their pKa nearer to 8 and enhance their therapeutic potential. 相似文献
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Joanna L. Mundy John M. Harrison Peter Watts Christopher M. Timperley 《Phosphorus, sulfur, and silicon and the related elements》2013,188(8):1847-1857
The synthesis of fifteen phosphylated compounds having P─OCD3, P─CD3, or P─OCD2CH3 groups is described. Selective chemistry for the precise placement of the deuterated groups was devised, and the products were isolated in a high purity in generally good yields. The compounds were required to study their behavior after electrospray ionization in an ion trap mass spectrometer. 相似文献
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S Rayaprol Krushna Mavani CM Thaker DS Rana Keka Chakravorty SK Paranjape M Ramanadham Nilesh A Kulkarni DG Kuberkar 《Pramana》2002,58(5-6):877-880
A new series of mixed oxide superconductors with the stoichiometric composition La2−x
Dy
x
Ca
y
Ba2Cu4+y
O
z
(x=0.0 − 0.5, y=2x) has been studied for structural and superconductiong properties. Our earlier studies on La2−x
(Y/Er)
x
Ca
y
Ba2Cu4+y
O
z
series, show a strong dependence of T
c on hole concentration (p
sh). In the present work, the results of the analysis of the neutron diffraction measurements at room temprerature on x=0.3 and 0.5 samples are reported. It is interesting to know that Ca substitutes for both La and Ba site with concomitant
displacement of La onto Ba site. Superconductivity studies show that maximum T
c is obtained for x=0.5, y=1.0 sample (T
c ∼ 75 K), for La1.5Dy0.5Ca1Ba2Cu5O
z
(La-2125). 相似文献
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