Correlation of the Physicochemical and Structural Properties of pDNA/Cationic Liposome Complexes with Their in Vitro Transfection

In this study, we characterized the conventional physicochemical properties of the complexes formed by plasmid DNA (pDNA) and cationic liposomes (CL) composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) (50/25/25% molar ratio). We found that these properties are nearly unaffected at the studied ranges when the molar charge ratio (R(±)) between the positive charge from the CL and negative charge from pDNA is not close to the isoneutrality region (R(±) = 1). However, the results from in vitro transfection of HeLa cells showed important differences when R(±) is varied, indicating that the relationships between the physicochemical and biological characteristics were not completely elucidated. To obtain information regarding possible liposome structural modifications, small-angle X-ray scattering (SAXS) experiments were performed as a function of R(±) to obtain correlations between structural, physicochemical, and transfection properties. The SAXS results revealed that pDNA/CL complexes can be described as being composed of single bilayers, double bilayers, and multiple bilayers, depending on the R(±) value. Interestingly, for R(±) = 9, 6, and 3, the system is composed of single and double bilayers, and the fraction of the latter increases with the amount of DNA (or a decreasing R(±)) in the system. This information is used to explain the transfection differences observed at an R(±) = 9 as compared to R(±) = 3 and 6. Close to the isoneutrality region (R(±) = 1.8), there was an excess of pDNA, which induced the formation of a fraction of aggregates with multiple bilayers. These aggregates likely provide additional resistance against the release of pDNA during the transfection phenomenon, reflected as a decrease in the transfection level. The obtained results permitted proper correlation of the physicochemical and structural properties of pDNA/CL complexes with the in vitro transfection of HeLa cells by these complexes, contributing to a better understanding of the gene delivery process.     

Luminescence lifetime distributions analysis in heterogeneous systems by the use of Excel's Solver

A detailed study of the luminescence decay curves of pyrene included within p-tert-butylcalix[4]arene cavities and benzophenone into silicalite channels is reported. A new methodology for a lifetime distribution analysis of the decay curve of probes onto heterogeneous surfaces is presented, which allows for asymmetric distributions and uses Voigt profiles (Gaussian and Lorentzian mixture) instead of pure Gaussian or Lorentzian distributions. Our approach uses a very simple and widely available tool for fitting, the Microsoft Excel Solver. In the case of the pyrene/tert-butylcalix[4]arene sample, the room temperature luminescence detected in the microsecond time scale was not only the phosphorescence of pyrene but also monomer delayed fluorescence, crystal phosphorescence, and excimer delayed fluorescence. In the benzophenone/silicalite case, three emissive forms of benzophenone could be assigned, one of benzophenone included into the silicalite circular zigzag channels, another for emplacement into the elliptical straight channels and finally when benzophenone is placed at the crossing points of those silicalite channels, where smaller spatial restrictions for benzophenone exist.     

Spinning rigid bodies driven by orbital forcing: the role of dry friction

Nonlinear Dynamics - A “circular orbital forcing” makes a chosen point on a rigid body follow a circular motion while the body spins freely around that point. We investigate this...     

High Performance of Carbon Nanotube Refrigerators

Vapor‐compression dominates the market for refrigeration devices due to low cost and relatively high efficiency. However, the most efficient vapor refrigerants are either ozone depleting or global warming substances. Solid‐state cooling is a young field of research with promising results toward the development of new, efficient, and environment friendly technology for a new generation of refrigeration devices. One of these methods is based on the so‐called elastocaloric effect (ECE), which consists of a temperature variation of a system in response to the application of adiabatic stresses. Although most of the literature describes the study of ECE solid‐state cooling based on materials undergoing phase‐transitions, a study recently predicted that carbon nanotubes (CNTs) present ECE as large as 30 K for 3% of strain. This motivates research toward the development of nanorefrigerators. As nobody knows the efficiency of such an ECE‐based CNT nanorefrigerator, here, significantly high coefficient of performance values of 4.1 and 6.5, and extracted heat per weight as large as 40 J g^?1 are reported for a zigzag CNT nanorefrigerator operating in an Otto‐like thermodynamic cycle. This efficiency is shown to overcome that of some other ECE materials.     

Analysis of lettuce evapotranspiration across soil water

In this study, we evaluated the lettuce accumulated evapotranspiration (ET) across four levels of irrigation, using a nonlinear mixed‐effects model. The plants were grown in protected environments and monitored over 23 consecutive days. When the moisture of the substrate in the vessels reached 50% of their maximum retention capacity, the water levels were elevated according to four treatments: , , , and . The model appeared to provide a good fit to the data and showed that the estimates of the maximum amount of accumulated ET were similar for the three treatments with soil water deficit and lower for . The results of the study supported the idea that optimization of the ET of lettuce plants could be achieved through irrigation with deficit, also indicating that the economical use of water was the most efficient way to boost agricultural production. Recommendations for resource managers

• The continued growth of the world population will result in a decrease of quality and availability of water and also an increase in demand for food. Therefore, sustainability will depend on high agricultural productivity with rational use of water.
• Considered the most efficient technology for boosting agricultural productivity, irrigation is also the largest water consumer in the world. With any kind of irrigation in the vegetable production area, the water intake for the plants must be treated with great caution.
• Both a lack or excess in water can decrease plant productivity. The amount of water available in the soil should be enough to maximize production. It is shown that high levels of irrigation are not necessary for this.
• It is important to balance the amount of irrigated water for an optimal level, in order that the production has its the production is maximized and valuable water resources are not wasted.