Recovery and sporicidal resistance of various B. subtilis spore preparations on porcelain penicylinders compared with results from AOAC test methods

Sporicidal test results obtained from carriers inoculated with 4 types of defined Bacillus subtilis spore preparations were compared with the standard AOAC sporicidal test using soil extract nutrient broth (SENB) B. subtilis 19659 spores. Recoveries of spores inoculated on penicylinders from B. subtilis clean spores (washed and suspended in water) and B. subtilis 19659 spores inoculated from culture filtrates according to the AOAC method were compared. Spores were exposed to 6 concentrations (0.5-3.0% w/v) of glutaraldehyde in phosphate buffer (pH 7.5) for 10 h. Concentrations were established by titrimetry and liquid chromatography. Recoveries of surviving spores were determined for 3 types of clean B. subtilis var. niger preparations, one clean B. subtilis 19659 preparation, and the SENB B. subtilis 19659 filtrates. Spore carriers, inoculated by the standard AOAC protocol, resulted in as much as a 2-log number difference in runs 1-12, but not more than 0.5 log number for each clean spore preparation. The SENB spores varied most in resistance to glutaraldehyde, with no growth in recovery media from 3 different batches of 1, 1.5, and 2% glutaraldehyde. Separate batches of SENB preparations of B. subtilis 19659 were resistant and destroyed by 1.0% glutaraldehyde, with 3.98 and 6.0 log numbers of spores on penicylinders, respectively. Clean spore preparations of B. subtilis 19659 on porcelain penicylinders were more resistant to glutaraldehyde than were SENB spores. Nutrient agar/Mg/Ca and nutrient agar/Mg spore preparations of B. subtilis var. niger showed the most uniform resistance to glutaraldehyde. Spores with calcium added showed increased resistance to glutaraldehyde. B. subtilis 19659 spores from the Columbia broth spore preparation were the most resistant and were recovered after exposure to 3.0% glutaraldehyde.     

The theory of non-Cottrellian diffusion on the surface of a sphere or truncated sphere.

A method is developed whereby spherical and other particles can be derivatised with electroactive species on their surface and then immobilised on the surface of an electrode. The chronoamperometric and voltammetric responses in the limit of reversible electrode kinetics are modelled using a theory of charge movement over the surface of the spheres where this movement is considered as a diffusional process. The model is extended to include different distributions of sphere radii and to model the scenario of truncated spheres resting on the electrode surface. It is found that a good estimation of the truncation angle can be found by fitting the experimental data with theoretical predictions.     

Dinuclear zinc(II) double-helicates of homochirally substituted bis(dipyrromethene)s

[structure: see text] A series of bis(dipyrromethene)s substituted with aromatic amide and aliphatic ester homochiral auxiliaries have been prepared and complexed with zinc(II) ions to form double-helical dinuclear complexes. CD analysis of the crude complexes revealed that the helicates formed in a diastereoselective manner. The helicates have been resolved into their constituent M and P helices by HPLC, indicating that the helical sense of the complexes is stable to racemization.     

Synthesis of some pyridylamino-1,4-disubstituted phthalazines

Two general procedures involving the condensation of phthalonitrile or 1,3-diiminoisoindoline with various aminopicolines, followed by ring expansion with hydrazine to the corresponding phthalazine are described. Syntheses are reported of 1, 4-di(3′-methyl-2′-pyridyl) aminophthalazine, 1,4-di(5′-methyl-2′-pyridyl)aminophthalazine, and 1,4-di(4′, 6′-dimethyl-2′-pyridyl)aminophthalazine.     

Improving splitless injection with electronic pressure programming

An experimental injection port has been designed for split or splitless sample introduction in capillary gas chromatography; the inlet uses electronic pressure control, in order that the column head pressure may be set from the GC keyboard, and the inlet may be used in the constant flow or constant pressure modes. Alternatively, the column head pressure may be programmed up or down during a GC run in a manner analogous to even temperature programming. Using electronic pressure control, a method was developed which used high column head pressures (high column flow rates) at the time of injection, followed by rapid reduction of the pressure to that required for optimum GC separation. In this way, high flow rates could be used at the time of splitless injection to reduce sample discrimination, while lower flow rates could be used for the separation. Using this method, up to 5 μl of a test sample could be injected in the splitless mode with no discrimination; in another experiment, 2.3 times as much sample was introduced into the column by using electronic pressure programming. Some GC peak broadening was observed in the first experiment.     

高锰酸钾-甲醛-噻枯唑化学发光体系测定噻枯唑

在酸性条件下,甲醛与高锰酸钾能够产生较弱的化学发光,而噻枯唑的存在能够大大地增强该化学发光强度。在一定浓度范围内,增加的发光强度与噻枯唑的浓度呈良好的线性关系,由此建立了测定噻枯唑的流动注射化学发光新方法。该方法的线性范围为0.03~0.8 mg/L和1.0~5.0 mg/L,检出限为8.9μg/L,对0.3 mg/L噻枯唑平行测定11次,其相对标准偏差为3.9%。方法用于测定环境水样中噻枯唑含量,回收率为95.4%~104.9%。     

Effects of high intensity ultrasound on inorganic solids

Ultrasonic irradiation dramatically affects the reactivity of a variety of inorganic solids. We have found, for example, large increases in the rates of intercalation of a wide range of compounds into various layered inorganic solids (such as ZrS2, V2O5, TaS2 and MoO3. High intensity ultrasound also enhances the heterogeneous catalysis of alkene hydrogenation by Ni powders. Scanning electron microscopy reveals that ultrasound has multiple effects on the morphology and surface characteristics of inorganic solids, creating substantial surface damage, increasing surface areas significantly and causing increased particle aggregation.     

Na_3AlF_6-Al_2O_3系熔盐离子结构的拉曼光谱研究

利用激光共焦拉曼光谱仪测定了不同氧化铝含量的Na3AlF6-Al2O3体系的常温与升温拉曼光谱,通过解析该拉曼光谱总结了其离子结构的种类以及随温度的变化规律,并对相应的拉曼谱峰进行了归属。结果表明当温度升至1173K及以上时,样品开始熔化并出现AlF63-→AlF4-的转化,同时伴有复杂离子的生成。将实验结果与模拟计算结合,发现在氧化铝含量较高的样品中存在络合离子Al2O2F42-。     

2-F-环氧丁烷与几种生物小分子间的赝氢键结构

采用密度泛函B3LYP(Becke,three-parameter,Lee-Yang-PaH)/6-311++G**和HF(Hartree Fock)/6-311++G**方法,从理论上探讨了2-F-环氧丁烷分别与几种常见而重要的生物小分子咪唑(Iminazole)、噻唑(Thiazole)和恶唑(Oxazo1e)等分子间的弱相互作用,发现分子间同时存在N…H常规氢键和C-F…H-C赝氢键结构.弱相互作用能计算表明3个复合物的相对稳定性相当.计算结果表明,在C-F…H-C赝氢键结构中,与电子的直接供体F密切相关的共价键C-F键长增大,伸缩振动的频率红移,而作为电子受体的H-C基团,其C-H键伸缩振动光谱蓝移;另外,电子密度拓扑性质表明C-F…H-C赝氢键的共价性及离子性均与常规氢键相当.     

三次Gauss和及二项指数和的混合均值

我们用三角和的性质研究一类三次Gauss和与两项指数和混合均值的计算问题,并给出一个精确的计算公式.