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101.
Le Bihan T Duewel HS Figeys D 《Journal of the American Society for Mass Spectrometry》2003,14(7):719-727
We have developed an on-line strong cation exchange (SCX)-ESI-MS/MS platform for the rapid identification of proteins contained in mixtures. This platform consists of a SCX precolumn followed by a nanoflow SCX column on-line with an electrospray ion trap mass spectrometer. We also used this platform to study the dynamics of peptide separation/extraction by SCX, in particular to understand the parameters affecting the performance of SCX in multidimensional chromatography. For example, we have demonstrated that the buffer typically used for tryptic digestion of protein mixtures can have a detrimental effect on the chromatographic behaviour of peptides during SCX separations, thereby affecting certain peptide quantitation approaches that rely on reproducible peptide fractionation. We have also demonstrated that band broadening results when a step (discontinuous) gradient approach is used to displace peptides from the SCX precolumn, reducing the separation power of SCX in multidimensional chromatography. In contrast, excellent chromatographic peak shapes are observed when a defined (continuous) gradient is used. Finally, using a tryptic digest of a protein extract derived from human K562 cells, we observed that larger molecular weight peptides are identified using this on-line SCX approach compared to the more conventional reverse phase (RP) LC/MS approach. Both methods used in tandem complement each other and can lead to a greater number of peptide identifications from a given sample. 相似文献
102.
Siham El FangourAlexandre Guy Jean-Pierre VidalJean-Claude Rossi Thierry Durand 《Tetrahedron letters》2003,44(10):2105-2108
The first synthesis of the two enantiomers of phytoprostane F1 methyl ester 1 and 2 is described using the syn-anti-syn alcoxy ester 3 as starting material. 相似文献
103.
Clément Ghiazza Vincent Debrauwer Dr. Cyrille Monnereau Lhoussain Khrouz Dr. Maurice Médebielle Dr. Thierry Billard Dr. Anis Tlili 《Angewandte Chemie (International ed. in English)》2018,57(36):11781-11785
The first visible‐light‐mediated synthesis of trifluoromethylselenolated arenes under metal‐free conditions is reported. The use of an organic photocatalyst enables the trifluoromethylselenolation of arene diazonium salts using the shelf‐stable reagent trifluoromethyl tolueneselenosulfonate at room temperature. The reaction does not require the presence of any additives and shows high functional‐group tolerance, covering a very broad range of starting materials. Mechanistic investigations, including EPR spectroscopy, luminescence investigations, and cyclic voltammetry allow rationalization of the reaction mechanism. 相似文献
104.
UV‐induced formation of pyrimidine dimers in DNA is a major deleterious event in both eukaryotic and prokaryotic cells. Accumulation of cyclobutane pyrimidine dimers and pyrimidine (6‐4) pyrimidone photoproducts can lead to cell death or be at the origin of mutations. In skin, UV induction of DNA damage is a major initiating event in tumorigenesis. To counteract these deleterious effects, all cell types possess DNA repair machinery, such as nucleotide excision repair and, in some cell types, direct reversion. Different analytical approaches were used to assess the efficiency of repair and decipher the enzymatic mechanisms. We presently review the information provided by chromatographic methods, which are complementary to biochemical assays, such as immunological detection and electrophoresis‐based techniques. Chromatographic assays are interesting in their ability to provide quantitative data on a wide range of damage and are also valuable tools for the identification of repair intermediates. 相似文献
105.
Matthew Myers Eric F. Connor Thierry Glauser Andreas Mck Gregory Nyce James L. Hedrick 《Journal of polymer science. Part A, Polymer chemistry》2002,40(7):844-851
A new metal‐free synthetic approach to the controlled ring‐opening polymerization (ROP) of lactide with nucleophilic phosphines as transesterification catalysts is described. P(Bu)3, PhPMe2, Ph2PMe, PPh3, and related phosphines are commercially available, inexpensive catalysts that generate narrowly dispersed polylactides with predictable molecular weights. These organic catalysts must be used in combination with an initiator, such as an alcohol, to generate an alcoholate ester α‐end group upon ROP. A likely polymerization pathway is through a monomer‐activated mechanism, with minimal active species, facilitating narrow molecular weight distributions. © 2002 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 40: 844–851, 2002; DOI 10.1002/pola.10168 相似文献
106.
Biosensors designed for environmental and food quality control based on screen-printed graphite electrodes with different configurations 总被引:3,自引:0,他引:3
Avramescu A Andreescu S Noguer T Bala C Andreescu D Marty JL 《Analytical and bioanalytical chemistry》2002,374(1):25-32
Graphite electrodes fabricated by screen-printing have been used as amperometric detectors in biosensors based on NAD(+)-dependent dehydrogenases, tyrosinase, or genetically modified acetylcholinesterases. The mono-enzyme sensors have been optimized as disposable or reusable devices for detection of a variety of substrates important in the food industry ( D-lactic acid, L-lactic acid, acetaldehyde) or in environmental pollution control (phenols and dithiocarbamate, carbamate and organophosphorus pesticides). The sensors were prepared in four configurations differing in enzyme confinement, enzyme immobilization and location of the immobilization agent in the biosensor assembly. Tests on real samples have been performed with the biosensors; D-lactic acid and acetaldehyde have been detected in wine and phenols in air. 相似文献
107.
Under Lewis acid activation, hemiaminals of trifluoroacetaldehyde and related (fluoroalkyl)aldehydes generate iminium species that can react with various nucleophiles to provide fluorinated amines. 相似文献
108.
109.
Improvements for ground-based remote sensing of atmospheric aerosol properties by additional polarimetric measurements 总被引:1,自引:0,他引:1
Zhengqiang Li Philippe Goloub Oleg Dubovik Luc Blarel Wenxing Zhang Thierry Podvin Alexander Sinyuk Mikhail Sorokin Hongbin Chen Brent Holben Didier Tanr Marius Canini Jean-Pierre Buis 《Journal of Quantitative Spectroscopy & Radiative Transfer》2009,110(17):1954-1961
We discuss the improvements in the aerosol properties characterization resulting from the additional multi-wavelength polarization measurements measured by a new CIMEL polarized sun/sky-photometer, CE318-DP. In order to process direct-sun, sky and polarization measurements in a wide spectral range (340–1640 nm), we developed new calibration methods and strategies, e.g. using the Langley plot method to calibrate both direct-sun irradiance and sky radiance, as well as combining laboratory facilities with a vicarious method to calibrate the polarized sky measurements. For studying the impact of new polarimetric measurements on the retrievals of aerosol properties, we have processed an extensive record of field measurements using an updated Dubovik and King retrieval algorithm [Dubovik O, Sinyuk A, Lapyonok T, Holben BN, Mishchenko MI, et al. Application of spheroid models to account for aerosol particle nonsphericity in remote sensing of desert dust. J Geophys Res 2006;111:D11208.]. A preliminary analysis shows that adding polarization in the inversion can reduce possible errors (notably for about 30% of our field cases) in the fine mode size distribution, real part of refractive index and particle shape parameter retrievals, especially for small particles. 相似文献
110.