Generation of multicolor vector Kerr solitons by cross-phase modulation, four-wave mixing, and stimulated Raman scattering

We numerically and experimentally show the existence of multicolor vector spatial solitons in a Kerr planar waveguide through the combined effects of cross-phase modulation, four-wave mixing, and stimulated Raman scattering. Mutual spatial guiding of the Raman-Stokes, anti-Stokes, and pump waves is achieved in the high-conversion regime mainly by cross-phase modulation and phase-matched four-wave mixing induced by a power imbalance between Stokes and anti-Stokes components, leading to the generation of a clear-cut sech-shape three-frequency spatial soliton.     

Generation of a broadband single-mode supercontinuum in a conventional dispersion-shifted fiber by use of a subnanosecond microchip laser

We report the experimental generation, simply by use of a subnanosecond microchip laser at 532 nm and a conventional dispersion-shifted fiber, of a supercontinuum that spans more than 1100 nm. We show by detailed spectral analysis that this supercontinuum originates from a preliminary four-wave mixing process with multimode phase matching and subsequent double-cascade stimulated Raman scattering and is transversely single mode as a result of Raman-induced mode competition. This technique is believed to be the simplest configuration that allows one to generate a stable supercontinuum.     

Réponse dynamique asymptotique de plaques minces linéairement piézoélectriques dans l'approximation quasi-électrostatiqueAsymptotic dynamic response of thin linearly piezoelectric plates in the quasi-electrostatic approximation

We exhibit four types of asymptotic dynamic behaviors of a piezoelectric plate according to the magnitudes of its thickness and density. To cite this article: T. Weller, C. Licht, C. R. Mecanique 332 (2004).     

Variations of secretome profiles according to conditioned medium preparation: The example of human mesenchymal stem cell‐derived adipocytes

One challenging point in analyzing cellular secretome collected as conditioned medium is cross‐contamination by cell culture media components, especially bovine serum proteins. A common approach for serum removal is to wash the cells, an alternative is to grow cells using serum‐free conditions. Given that the sample processing may influence the phenotype of cells and thus the secretome, it is important to establish the optimal protocol for each cell type. In this study, we compared two methods for preparing conditioned medium from human adipocytes derived from mesenchymal stem cells. Cells were either washed twice with PBS or cultured the last four days of differentiation in serum‐free adipogenic medium. Gene expression of the cells was evaluated by using real‐time PCR and 1D LC‐MS/MS was used to compare secreted proteins present in the culture supernatants. Surprisingly, results showed significant differences in gene expression patterns of the cells and in protein content of the conditioned media and suggested that PBS washes induced severe modifications of the phenotype of cells and thus changes in protein secretion profiles. These data emphasize the significant variations in protein species related to cell manipulations and underline the importance of procedure optimization prior to any proteomic investigation.     

Synthesis of Thiadiazolylaminoglycosides Through Ring Contraction of Triazinoglycosides Induced by [TBA‐Ox]

A new synthetic route to thiadiazolylaminoglycosides, by a ring expansion/ring contraction sequence of glycosyl triazines, has been developed. Two potential mechanisms of this oxidative ring contraction using [TBA‐Ox] are discussed. The more plausible involves formation of an oxatriazepane intermediate followed by loss of formic acid, ring opening, and subsequent recyclization.     

Template directed synthesis of antibody Fc conjugates with concomitant ligand release

Antibodies are an attractive therapeutic modality for cancer treatment as they allow the increase of the treatment response rate and avoid the severe side effects of chemotherapy. Notwithstanding the strong benefit of antibodies, the efficacy of anti-cancer antibodies can dramatically vary among patients and ultimately result in no response to the treatment. Here, we have developed a novel means to regioselectively label the Fc domain of any therapeutic antibody with a radionuclide chelator in a single step chemistry, with the aim to study by SPECT/CT imaging if the radiolabeled antibody is capable of targeting cancer cells in vivo. A Fc-III peptide was used as bait to bring a carbonate electrophilic site linked to a metal chelator and to a carboxyphenyl leaving group in close proximity with an antibody Fc nucleophile amino acid (K317), thereby triggering the covalent linkage of the chelator to the antibody lysine, with the concomitant release of the carboxyphenyl Fc-III ligand. Using CHX-A′′-DTPA, we radiolabeled trastuzumab with indium-111 and showed in biodistribution and imaging experiments that the antibody accumulated successfully in the SK-OV-3 xenograft tumour implanted in mice. We found that our methodology leads to homogeneous conjugation of CHX-A′′-DTPA to the antibody, and confirmed that the Fc domain can be selectively labeled at K317, with a minor level of unspecific labeling on the Fab domain. The present method can be developed as a clinical diagnostic tool to predict the success of the therapy. Furthermore, our Fc-III one step chemistry concept paves the way to a broad array of other applications in antibody bioengineering.

A method is reported to attach a radionuclide chelator in a single step chemistry to the Fc domain of any therapeutic antibody.     

Structural Variations in the Central Heterocyclic Scaffold of Tripartite 2,6-Difluorobenzamides: Influence on Their Antibacterial Activity against MDR Staphylococcus aureus

Five series of heterocyclic tripartite 2,6-difluorobenzamides, namely 1,2,3-triazoles, 1,2,4- and 1,3,4-oxadiazoles, analogs of reported model anti-staphylococcal compounds, were prepared. The purpose was to investigate the influence of the nature of the heterocyclic central scaffold on the biological activity against three strains of S. aureus, including two drug-resistant ones. Among the 15 compounds of the new collection, a 3-(4-tert-butylphenyl)-1,2,4-oxadiazole linked via a methylene group with a 2,6-difluorobenzamide moiety (II.c) exhibited a minimal inhibitory concentration between 0.5 and 1 µg/mL according to the strain. Subsequent studies on II.c demonstrated no human cytotoxicity, while targeting the bacterial divisome.     

Vitamin B12: How the Problem of Its Biosynthesis Was Solved

Vitamin B₁₂ is an essential vitamin for human health, and lack of it leads to pernicious anemia. This biological activity has attracted intense interest for some time; in addition, the complex architecture of the B₁₂ molecule has fascinated chemists and biochemists since its discovery as the first natural organocobalt complex and the establishment of its structure by X-ray analysis. The organic ligand surrounding the cobalt displays many stereogenic centers along its periphery carrying reactive functional groups. This complexity led vitamin B₁₂ to be rightly regarded as an extreme challenge to the synthetic chemist. Yet microorganisms achieve this synthesis in vivo with complete control of regio- and stereochemistry. How do they do it? This review tells the full remarkable story. Success in unraveling this biosynthetic puzzle resulted from a collaborative effort by biologists and chemists using the full range of methods available from their disciplines–from genetics at one end of the spectrum to synthesis and NMR spectroscopy at the other. This work can act as a guide for future research on the biosynthesis of yet more complex natural substances.