Sirtuin 1 (SIRT1) is a nicotinamide adenine dinucleotide-dependent histone deacetylase that plays diverse physiological roles. However, little is known about the regulation of SIRT1 activity. Here, we show that phospholipase D2 (PLD2), but not PLD1, selectively interacts with SIRT1 and increases the deacetylase activity of SIRT1. PLD2 does not interact with the other isozymes of SIRT (SIRT2–7). Two leucine residues in the LXXLL motif (L173 and L174) in the phox domain of PLD2 interact with the region essential for SIRT1 activity. PLD2 stimulates the SIRT1-mediated deacetylation of p53 independent of its lipase activity. In our study, mutagenesis of the LXXLL motif suppressed the interaction of PLD2 with SIRT1 and inhibited SIRT1-mediated p53 deacetylation and p53-induced transactivation of proapoptotic genes. Ultimately, overexpression of wild-type PLD2 but not that of LXXLL-mutant PLD2 protected cells against etoposide-induced apoptosis. Moreover, PLD2 did not protect against apoptosis induced by SIRT1 depletion under genotoxic stress. Collectively, our results suggest that PLD2 is a positive regulator of SIRT1 and modulates p53-mediated apoptosis via SIRT1.Subject terms: Lipid signalling, Cancer metabolism相似文献
Self‐doped sulfonated polyaniline (PSA) has been synthesized on the surface of micellar nanoparticles made from positively charged surfactants by biocatalysis. The conformation forced by the electrostatic charge interactions between the positively charged micelle and the negatively charged PSA increases the conductivity of the PSA by three orders of magnitude. The pure PSA recovered from ion exchange, however, shows quite similar electrical properties compared with sulfonated polyanilines reported earlier. The increased conductivity of PSA complexes is as a result of the increased charge carrier concentration caused by a certain conformational locking.
We revisit the protocols to create maximally entangled states between two Josephson junction (33) charge phase qubits coupled to a microwave field in a cavity as a quantum data bus. We analyze a novel mechanism of quantum decoherence due to the adiabatic entanglement between qubits and the data bus, the off-resonance microwave field. We show that even if the variable of the data bus can be adiabatically eliminated, the entanglement between the qubits and data bus remains and can decohere the superposition of two-particle state. Fortunately we can construct a decoherencefree subspace of two-dimension to against this adiabatic decoherence. To carry out the analytic study for this decoherence problem, we develop Frohlich transformation to re-derive the effective Hamiltonian of these systems, which is equivalent to that obtained from the adiabatic elimination approach. 相似文献
Polyethylene(PE)/clay nanocomposites have been successfully prepared by in situ polymerization with an intercalation catalyst titanium-montmorillonite (Ti-MMT) and analyzed by X-ray diffraction analysis (XRD), Fourier transform infrared analysis (FT-IR), Transmission electron microscopy (TEM), differentail scanning calorimetry (DSC), thermal gravimetric analysis (TGA) and tensile testing. XRD and TEM indicate that the clay is exfoliated into nanometer size and disorderedly dispersed in the PE matrix, and the PE crystallinity of PE/clay nanocomposite declines to 15∼30%. Compared with pure PE, PE/clay nanocomposites behave higher thermal, physical and mechanical properties; the layer structure of the clay decreases the polymerization activity and produce polymer with a high molecular weight. For PE/clay nanocomposites, the highest tensile strength of 33.4 MPa and Young's modulus of 477.4 MPa has been achieved when clay content is 7.7 wt %. The maximum thermal decomposition temperature is up to 110 °C higher, but the thermal decomposition temperature of the PE/clay nanocomposites decreases with the increases of the clay contents in the PE matrix. 相似文献
Based on the modified spectrum, the analytic expressions for the
variance and normalized covariance of angle-of-arrival (AOA)
fluctuations are presented, which are applicable to the weak and
strong regimes. The experimental data of AOA fluctuations validate
the new derived expressions in weak and strong regimes. The results
show that the receiving aperture D, outer scale and cell scale
larger than the scattering disc S contribute significantly to the
AOA fluctuations, and contributions from the small-scale turbulence
are negligible. For the case of 4S/D\ll 1, the receiving aperture
dominates low-pass filtering effects and the new displacement
variances are in good agreement with the results from the
old weak-fluctuation theory. For the case of 4S/D\gg 1, the scattering disc dominates the low-pass
filtering effects and the new displacement variances depart from the
results from the old
weak-fluctuation theory. 相似文献
Completely solving the dissipative dynamics of nonlinear Jaynes-Cumming model is a very difficult task.In our recent work (Phys. Lett. A284 (2001) 156), we just obtained analytical results of the field dissipative dynamics of the nonlinear JCM. In the present paper, employing the perturbative expansion of master equation, we obtain the density operator of the system (field +atom). The coherence losses of the system and of the atom are investigated when two-photon process is involved. We also study the effect of different atomic initial states and the influence of the field amplitude on the atomic coherence loss. 相似文献
The convergence problem of the family of Euler-Halley methods is considered under the Lipschitz condition with the L-average,and a united convergence theory with its applications is presented. 相似文献