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Marcela S. O. Wanderley Carla Oliveira Danyelly Bruneska Lucília Domingues José L. Lima Filho José A. Teixeira Solange I. Mussatto 《Chemical Papers》2013,67(7):682-687
Frutalin, a galactose-specific lectin used to detect specific tumour markers, is a protein with low expression level in breadfruit. In the present study, fed-batch fermentation in a stirred tank bioreactor was used as a strategy to enhance protein production by a recombinant Pichia pastoris KM71H. By using this process, the production of recombinant frutalin was 4-fold higher than the value obtained in shaker flasks batch assays. Supplementation of the fermentation medium with trace elements (Pichia trace minerals, PTM) was also evaluated in order to stimulate production of the recombinant protein. The addition of PTM to the minimum medium afforded a recombinant protein production of 13.4 mg L?1, which was 2.5-fold higher than that achieved from the culture medium without PTM supplementation. These results are significant as the development of strategies to improve the production of recombinant frutalin may broaden its application in cancer diagnosis. 相似文献
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J. M. Teixeira Pinto F. Touchard S. Castagnet C. Nadot-Martin D. Mellier 《Experimental Mechanics》2013,53(8):1311-1321
To improve physical motivation of mechanical modeling for semi-crystalline polymers, a better understanding of micro-mechanisms and a quantification of the mechanical response at the microstructure scale are needed. Strain field evolutions during deformation would be useful information but experimental techniques are still lacking for these materials. In this paper, an in-situ Digital Image Correlation (DIC) technique in the Scanning Electron Microscope (SEM) was developed to access strain fields at the spherulitic scale of a polypropylene. The evolution of strain heterogeneities during deformation were analyzed and correlated to the microstructure. 相似文献
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Tanay Chattopadhyay Cláudia Reis Paulo André António Teixeira 《Optics Communications》2012,285(9):2266-2275
A novel scheme for an all-optical clocked D flip-flop, with very low complexity, is proposed and numerically demonstrated. This new flip-flop configuration is based on a semiconductor optical amplifier — Mach–Zehnder interferometer (SOA-MZI), with a feedback loop, and presents two stable states determined by the phase shift between the two MZI arms. 相似文献
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Jaume Llibre Paulo R. da Silva Marco A. Teixeira 《Journal of Dynamics and Differential Equations》2012,24(1):1-12
In this article we establish an interaction between non-smooth systems, geometric singular perturbation theory and synchronization
phenomena. We find conditions for a non-smooth vector fields be locally synchronized. Moreover its regularization provide
a singular perturbation problem with attracting critical manifold. We also state a result about the synchronization which
occurs in the regularization of the fold-fold case. We restrict ourselves to the 3-dimensional systems (ℓ = 3) and consider the case known as a T-singularity. 相似文献
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Luís Antônio Dantas Silva Fernanda Vieira Teixeira Raphael Caixeta Serpa Najla Locatelli Esteves Rayane Ramos dos Santos Eliana Martins Lima Marcílio Sérgio Soares da Cunha-Filho Adriano Antunes de Souza Araújo Stephânia Fleury Taveira Ricardo Neves Marreto 《Journal of Thermal Analysis and Calorimetry》2016,123(3):2337-2344
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do Nascimento Marreiro Teixeira Ana Siqueira Siqueira Teixeira Paulo Ronaldo Sousa de Oliveira Farias Emanuel Airton Ferraz e Sousa Brandon Moura Sérvulo Kátia Bonfim de Leite da Silva Durcilene Alves Eiras Carla 《Journal of Solid State Electrochemistry》2020,24(5):1143-1155
Journal of Solid State Electrochemistry - The present work proposes a new electrochemical sulfite biosensor based on babassu mesocarp nanoparticles (BMNPs) immobilized on the surface of a pyrolytic... 相似文献
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