Frequency conversion in laser-produced boron plasma using longitudinal pump scheme

The high-order harmonic generation from boron plasma was studied using the longitudinal pump scheme. The harmonics up to the 57th order (λ=13.96 nm) were observed. The characteristics of plateau in the range of 15th to 57th harmonics were analyzed depending on fundamental radiation intensity and the saturation of harmonic cutoff was observed. The harmonic cutoff energy was restricted by the self-defocusing of femtosecond radiation. A comparison of longitudinal and orthogonal pump schemes of boron plasma excitation was presented.     

Ultrafast diagnosis of the genetic-related disorders using the combined technologies of multiplex PCR and multichannel microchip electrophoresis.

For the diagnosis of unexplained male infertility a multiplex PCR for 6 markers, which are well-known as candidate genes for studying male infertility and located on the human Y-chromosome, has been designed. The multiplex PCR products have been separated on a 12 channel microchip electrophoresis system, which can analyze different samples simultaneously. By combining the technologies of multiplex PCR with multichannel microchip electrophoresis, the number of the DNA markers that can be screened simultaneously is increased to be 72 marker (12 x 6) in a single run while the electrophoresis analysis time is reduced to be only 180 s.     

Microchip electrophoresis for specific gene detection of the pathogenic bacteria V. cholerae by circle-to-circle amplification.

We have developed a new method for a fast and precise analysis of circle-to-circle amplification (C2CA) product for specific gene detection by microchip electrophoresis. In this method, we have added a new enzymatic step to the C2CA reaction, which could be carried out isothermally at 37 degrees C. Compared to the original single-stranded DNA, the double-stranded DNA that is produced by this enzymatic reaction is more reliable for analysis by microchip electrophoresis. C2CA product was detected within 55 s with high reproducibility by this method which was successfully applied to the detection of 10-ng genomic DNA of the pathogenic bacteria Vibrio. cholerae within 110 s. Purification was found to be an indispensable step for the analysis of the C2CA product of genomic DNA samples.     

Einflu? des pH-Wertes und der Salzkonzentration auf die Helix-Kn?uel-Umwandlung von Poly A nach einer modifizierten DSC-Methode

Ohne Zusammenfassung     

Studies on the biotransformation of paeonol by means of isotope tracer techniques.--Synthesis and physicochemical properties of carbon-13 and deuterium labeled compounds--

In order to perform the metabolic study of paeonol (I) in human and animals by using isotope tracer techniques, synthesis of I and its urinary metabolites labeled with carbon-13 or deuterium was investigated. 2-Hydroxy-4-methoxy[d3]acetophenone, 2,5-dihydroxy-4-methoxy[d3]acetophenone, and resacetophenone[acetyl-13C2] were synthesized from methyl iodide-d3, dimethyl sulfate-d6, and acetic acid-1,2-13C2, respectively. Physicochemical studies by IR and NMR spectra showed that stable isotope was not eliminated during the synthetic process of each compound. For the quantification of the metabolites by GC-MS analysis, isotope effect on gas chromatography and fragmentation was studied.     

In-beam gamma-ray and electron spectroscopy following the75As(p,n)75Se reaction

The level scheme of⁷⁵Se has been studied through the⁷⁵As (p, n) reaction at proton energies from 1.5 to 5.0 MeV.γ-ray and internal conversion electron measurements were made using NaI (T1) and Ge(Li) detectors and a six-gap electron spectrometer. A proportional counter and a thin window NaI(T1) detector were used to detectγ-rays with energies less than 30 keV. The level scheme has been established by observing the thresholds of variousγ-rays and byγ-γ and e^?-γ coincidence measurements. New levels at 133.0, 293.2, 790.0, 953.0, 1020.8, 1184.3, 1198.5 and 1258.2 keV not observed in earlier (p, n) studies have been established. Conversion coefficients of most of the low-lying transitions have been determined. Angular distributions of some of theγ-rays were also measured and compared with the statistical model calculations. DefiniteJ ^π assignments have been made to most of the low-lying levels. Life-times of the 112.1, 133.0, 286.7 and 293.2 keV levels have been measured to be 0.69±0.12, 5.3±0.6, 1.35±0.15 and 31±2 nsec respectively. The reduced transition probabilities for various low-lying transitions have been determined and compared with recent calculations. The 1/2^? and 9/2⁺ levels hitherto unknown in this nucleus has been identified. The structure of the low-lying levels is discussed in terms of the existing models.     

Cycloaddition of oxetane and carbon dioxide catalyzed by tetraphenylstibonium iodide

Trimethylene carbonate was readily obtained in the reaction of oxetane and carbon dioxide in the presence of tetraphenylstibonium iodide.     

Phase unwrapping method along radial coordinates for speckle image reconstruction

We describe a phase estimating technique for a stellar speckle image reconstruction, which is based on the phase unwrapping method in polar coordinates. The phase unwrapping along radial lines is favorable to reduce accumulating errors in the phase estimation. This method is combined with Bruck-Sodin method which gives the estimate of the modulus of an object transform without a point reference source. Computer simulations of the image reconstruction are presented.