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排序方式: 共有124条查询结果,搜索用时 15 毫秒
41.
Urano Y Kamiya M Kanda K Ueno T Hirose K Nagano T 《Journal of the American Chemical Society》2005,127(13):4888-4894
Fluorescence imaging is the most powerful technique currently available for continuous observation of dynamic intracellular processes in living cells. Suitable fluorescence probes are naturally of critical importance for fluorescence imaging, but only a very limited range of biomolecules can currently be visualized because of the lack of flexible design strategies for fluorescence probes. At present, design is largely empirical. Here we show that the carboxylic group of traditional fluorescein dyes, formerly considered indispensable, has been replaced with other substituents, affording various kinds of new fluoresceins. Further, by breaking out of the traditional structure of fluorescein, we developed the first and totally rational design strategy for novel fluorescence probes based on a strict photochemical basis. The value of this approach is exemplified by its application to develop a novel, highly sensitive, and membrane-permeable fluorescence probe for beta-galactosidase, which is the most widely used reporter enzyme. 相似文献
42.
Konno T Haneishi K Hirotsu M Yamaguchi T Ito T Yoshimura T 《Journal of the American Chemical Society》2003,125(31):9244-9245
Treatment of fac(S)-[Rh(aet)3] (aet = 2-aminoethanethiolate) with aqueous HBF4 in air led to the protonation at coordinated thiolato groups to give a rhodium(III) dimer, [{Rh(aet)2(Haet)}{Rh(aet)(Haet)2}](BF4)3 ([1](BF4)3). On the other hand, similar treatment of fac(Se)-[Rh(aes)3] (aes = 2-aminoethaneselenolate) produced a dinuclear rhodium(III) complex, [Rh2(selenocystamine)3](BF4)6 ([2](BF4)6), because of the autoxidation of coordinated selenolato groups by air. The crystal structures of [1](BF4)3, DeltaDelta-[1](BF4)3, and [2](BF4)6 were determined by X-ray crystallography. In [1]3+ two RhIII octahedrons are connected through a strong triple thiol-thiolate S-H...S hydrogen bond, while two RhIII octahedrons are directly joined by a triple diselenide bond in [2]6+. The cyclic voltammetry indicated that in acidic media the RhIII center in fac(Se)-[Rh(aes)3] is more easily oxidized to RhIV than that in fac(S)-[Rh(aet)3], which is responsible for the formation of coordinated diselenide bonds. 相似文献
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Sakai K Ishigami E Konno Y Kajiwara T Ito T 《Journal of the American Chemical Society》2002,124(41):12088-12089
The first/second examples of partially oxidized 1-D platinum chain compounds consisting of cationic dimer units have been obtained from electro-oxidation of an aqueous solution containing cis-[Pt(NH3)2(OH2)2]2+ and acetate/propionate. The analytical and crystallographic studies reveal the mixed-valency of Pt(2.2+)infinity. The XPS confirms the presence of both Pt(II) and Pt(III). The solid-state physical measurements reveal that they are diamagnetic semiconductors and display a fairly broad, low-energy absorption band in the range of 500-3200 nm. 相似文献
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We present a theoretical model of the behavior of a concentrated electrorheological fluid (ERF) which explicitly takes into account the effects of conductivity. The increase in shear viscosity under an electric field is due to a layered structure between the electrodes, made up of the remnants of particle chains adhering to the electrodes by electrostatic image forces, and a freely flowing liquid layer where all the shear flow is concentrated. This layered model can explain the variation of electric current with shear rate, as well as the rheological response of a dynamic yield stress proportional to the square of the applied electric field. 相似文献
48.
Koichi Inoue Nuntawat Prayoonhan Haruhito Tsutsui Tasuku Sakamoto Maiko Nishimura Toshimasa Toyo'oka 《Journal of separation science》2013,36(8):1356-1361
Dichlorprop is available for agricultural use as a chiral pesticide. In this study, the stereoselective determination of dichlorprop enantiomers in tea samples such as green, black, jasmine, and oolong was developed by ultra performance LC with fluorescence spectrometry after covalent chiral derivatization. The separation was achieved on an Acquity BEH C18 column with the mobile phase consisting of 0.1% formic acid in acetonitrile/water at a flow rate of 0.4 mL/min. In the covalent chiral derivatization using (S)‐(+)‐4‐(N,N‐dimethylaminosulfonyl)‐7‐(3‐aminopyrrolidin‐1‐yl)‐2,1,3‐benzoxadiazole, the peak resolution between the S and R‐dichlorprop enantiomers was 2.6. LODs and LOQs values were 10 and 50 ng/mL standard solution. The linearity of the calibration curves yielded the coefficients (r2 > 0.99, ranging from 0.05 to 5 μg/mL) of determination of each of the dichlorprop enantiomers. SPE extraction was used for the sample preparation of dichlorprop in various tea samples. Recoveries were in the range of 82.4–97.6% with associated precision values (within‐day: 82.4–95.8%, n = 6, and between‐day: 83.7–97.6% for 3 days) for repeatability and reproducibility. Based on this result, our method has been proven to be highly efficient and suitable for the routine assay of dichlorprop enantiomers in various tea samples. We propose that the ultra performance LC assay after covalent chiral derivatization would be the renewed tools in the era of chiral stationary platform for chiral pesticide residues in foods. 相似文献
49.
Sasakura K Hanaoka K Shibuya N Mikami Y Kimura Y Komatsu T Ueno T Terai T Kimura H Nagano T 《Journal of the American Chemical Society》2011,133(45):18003-18005
Hydrogen sulfide (H(2)S) has recently been identified as a biological response modifier. Here, we report the design and synthesis of a novel fluorescence probe for H(2)S, HSip-1, utilizing azamacrocyclic copper(II) ion complex chemistry to control the fluorescence. HSip-1 showed high selectivity and high sensitivity for H(2)S, and its potential for biological applications was confirmed by employing it for fluorescence imaging of H(2)S in live cells. 相似文献
50.
Selective Ablation of β‐Galactosidase‐Expressing Cells with a Rationally Designed Activatable Photosensitizer 下载免费PDF全文
Dr. Yuki Ichikawa Dr. Mako Kamiya Dr. Fumiaki Obata Prof. Masayuki Miura Dr. Takuya Terai Dr. Toru Komatsu Dr. Tasuku Ueno Dr. Kenjiro Hanaoka Prof. Tetsuo Nagano Prof. Yasuteru Urano 《Angewandte Chemie (International ed. in English)》2014,53(26):6772-6775
We have developed an activatable photosensitizer capable of specifically inducing the death of β‐galactosidase‐expressing cells in response to photoirradiation. By using a selenium‐substituted rhodol scaffold bearing β‐galactoside as a targeting substituent, we designed and synthesized HMDESeR‐βGal, which has a non‐phototoxic spirocyclic structure owing to the presence of the galactoside moiety. However, β‐galactosidase efficiently converted HMDESeR‐βGal into phototoxic HMDESeR, which exists predominantly in the open xanthene form. This structural change resulted in drastic recovery of visible‐wavelength absorption and the ability to generate singlet oxygen (1O2). When HMDESeR‐βGal was applied to larval Drosophila melanogaster wing disks, which express β‐galactosidase only in the posterior region, photoirradiation induced cell death in the β‐galactosidase‐expressing region with high specificity. 相似文献