Determination of Iron in Tap and Waste Water Using Liquid–Liquid Extraction in a Flow-Injection System

A flow-injection procedure for the determination of iron(III) in water is described. The procedure is based on the formation of an ion pair between the tetraphenylarsonium (Ph₄As⁺) (TPA) or tetrabutylammonium (But₄N⁺) (TBA) cations and the tetrathiocyanatoferrate(III) complex (TTF). This ion pair is extracted with chloroform, and the absorbance of the organic phase is measured at 503nm (for Ph₄As⁺) or 475nm (for But₄N⁺). Iron concentrations higher than 0.9×10^–6molL^–1 (50µgL^–1) can be detected in the first case, with a relative standard deviation of 1.9% (n=12), a linear application rangeof between 1.34 and 54.0×10^–6molL^–1 (75–3015µgL^–1), and a sampling frequency of 30h^–1. For the ion pair with But₄N⁺, the detection limit is 0.52×10^–6molL^–1 (29µgL^–1), with a relative standard deviation of 1.6% and a linear application range between 0.73 and 54.0×10^–6molL^–1. Under the proposed working conditions, only Pd(IV), Cu(II) and Bi(III) interfere. With the application of the merging zones technique, considerable amounts of organic reagent can be saved. The TBA method was applied to the analysis of iron(III) in tap and industrial waste waters.     

Local Photodynamic Therapy Reduces Tissue Hyperplasia in an Experimental Restenosis Model

Abstract— Local photodynamic therapy may have potential in preventing myointimal hyperplasia after angioplasty. In this study, the effect of photodynamic therapy was evaluated in an experimental model of restenosis. Standardized unidirectional arterial injury with a directional atherectomy catheter was performed in porcine arteries. Animals were randomly allocated to four groups: group 1, unidirectional injury only; group 2, injury followed by local delivery of photosensitizer; group 3, injury followed by local exposure to monochromatic light; and group 4, where injury was followed by local drug delivery of photosensitizer and subsequent exposure to light (photodynamic therapy). Seven, 14 or 21 days after treatment, all experimental vessels were excised, fixed and processed for histology. An inflammatory and myoproliferative response was observed after injury in vessels from groups 1, 2 and 3. In group 4, after injury followed by photodynamic therapy, the myoproliferative response was significantly reduced. Thus, in this study, tissue hyperplasia after unidirectional injury was effectively suppressed by photodynamic therapy.     

Carrier ampholyte-free solution isoelectric focusing as a prefractionation method for the proteomic analysis of complex protein mixtures

The field of proteomics requires methods that offer high sensitivity and wide dynamic range. One of the strategies used to improve the dynamic range is sample prefractionation, such as microsolution isoelectric focusing (IEF). We have modified a commercial solution IEF instrument, the Rotofor, to prefractionate protein mixtures by carrier ampholyte-free solution IEF. The focusing chamber of the Rotofor was divided into several compartments by polyacrylamide membranes with imbedded Immobiline mixtures of specific pH values. When an electric field is applied, each protein migrates to the compartment confined by membranes with pH values flanking its isoelectric point. The approach was demonstrated for the focusing of myoglobin into a predicted compartment, as well as the separation of a complex soluble yeast protein mixture into several distinct fractions. The proteins were dissolved in water or 30% isopropanol. The method is applicable to both gel-based and solution-phase protein identification methods, without the need for further sample preparation.     

A nonlinear programming approach to lower bounds for the ground‐state energy of helium

Lower‐bound estimates for the ground‐state energy of the helium atom are determined using nonlinear programming techniques. Optimized lower bounds are determined for single‐particle, radially correlated, and general correlated wave functions. The local nature of the method employed makes it a very severe test of the accuracy of the wave function. ©1999 John Wiley & Sons, Inc. Int J Quant Chem 71: 455–463, 1999     

A fourth‐order method for numerical integration of age‐ and size‐structured population models

In many applications of age‐ and size‐structured population models, there is an interest in obtaining good approximations of total population numbers rather than of their densities. Therefore, it is reasonable in such cases to solve numerically not the PDE model equations themselves, but rather their integral equivalents. For this purpose quadrature formulae are used in place of the integrals. Because quadratures can be designed with any order of accuracy, one can obtain numerical approximations of the solutions with very fast convergence. In this article, we present a general framework and a specific example of a fourth‐order method based on composite Newton‐Cotes quadratures for a size‐structured population model. © 2008 Wiley Periodicals, Inc. Numer Methods Partial Differential Eq, 2009     

The advantage of knowing where to listen

This study examined the role of focused attention along the spatial (azimuthal) dimension in a highly uncertain multitalker listening situation. The task of the listener was to identify key words from a target talker in the presence of two other talkers simultaneously uttering similar sentences. When the listener had no a priori knowledge about target location, or which of the three sentences was the target sentence, performance was relatively poor-near the value expected simply from choosing to focus attention on only one of the three locations. When the target sentence was cued before the trial, but location was uncertain, performance improved significantly relative to the uncued case. When spatial location information was provided before the trial, performance improved significantly for both cued and uncued conditions. If the location of the target was certain, proportion correct identification performance was higher than 0.9 independent of whether the target was cued beforehand. In contrast to studies in which known versus unknown spatial locations were compared for relatively simple stimuli and tasks, the results of the current experiments suggest that the focus of attention along the spatial dimension can play a very significant role in solving the "cocktail party" problem.     

Direct magnitude estimation and interval scaling of pleasantness and severity in dysphonic and normal speakers

The purpose of this study was to determine the validity of voice pleasantness and overall voice severity ratings of dysphonic and normal speakers using direct magnitude estimation (DME) and equal-appearing interval (EAI) auditory-perceptual scaling procedures. Twelve naive listeners perceptually evaluated voice pleasantness and severity from connected speech samples produced by 24 adult dysphonic speakers and 6 normal adult speakers. A statistical comparison of the two auditory-perceptual scales yielded a linear relationship representative of a metathetic continuum for voice pleasantness. A statistical relationship that is consistent with a prothetic continuum was revealed for ratings of voice severity. These data provide support for the use of either DME or EAI scales when making auditory-perceptual judgments of pleasantness, but only DME scales when judging overall voice severity for dysphonic speakers. These results suggest further psychophysical study of perceptual dimensions of voice and speech must be undertaken in order to avoid the inappropriate and invalid use of EAI scales used in the auditory-perceptual evaluation of the normal and dysphonic voice.     

Phase IIb randomized trial of adjunct immunotherapy in patients with first-diagnosed tuberculosis,relapsed and multi-drug-resistant (MDR) TB

Placebo-controlled, randomized, phase 2b trial was conducted in 34 adults comprising 18 first-diagnosed (52.9%), 6 relapsed (17.6%), and 10 MDR-TB (29.4%) cases to investigate the safety and efficacy of an oral immune adjunct (V5). The immunotherapy (N = 24) and placebo (N = 10) arms received once-daily tablet of V5 or placebo for one month in addition to conventional anti-TB therapy (ATT) administered under directly observed therapy (DOT).     

A general solution method for an anti-plane shear crack dynamically accelerating along a bimaterial interface

We develop a general solution method for a dynamically accelerating crack under anti-plane shear conditions along the interface between two different homogeneous isotropic elastic materials. The crack is initially at rest, and after loading is applied the crack-tip speed which may accelerate up to the shear wave speed of the more compliant material. The analysis includes an exact, closed-form expression for the stress intensity factor for an arbitrary time-dependent crack-face traction, as well as expressions for computing the crack-face displacements and the stress in front of the crack. We also present some numerical examples for fixed loads and for loads moving with the crack tip, using a stress intensity factor fracture criterion, in order to examine the predicted effect of material mismatch on interfacial fracture.