全文获取类型
收费全文 | 501篇 |
免费 | 45篇 |
国内免费 | 1篇 |
专业分类
化学 | 469篇 |
力学 | 4篇 |
数学 | 41篇 |
物理学 | 33篇 |
出版年
2023年 | 8篇 |
2022年 | 13篇 |
2021年 | 14篇 |
2020年 | 29篇 |
2019年 | 29篇 |
2018年 | 13篇 |
2017年 | 5篇 |
2016年 | 24篇 |
2015年 | 21篇 |
2014年 | 39篇 |
2013年 | 22篇 |
2012年 | 50篇 |
2011年 | 40篇 |
2010年 | 32篇 |
2009年 | 26篇 |
2008年 | 32篇 |
2007年 | 24篇 |
2006年 | 24篇 |
2005年 | 27篇 |
2004年 | 17篇 |
2003年 | 10篇 |
2002年 | 14篇 |
2001年 | 10篇 |
2000年 | 6篇 |
1999年 | 5篇 |
1998年 | 4篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1993年 | 2篇 |
1976年 | 1篇 |
1975年 | 1篇 |
排序方式: 共有547条查询结果,搜索用时 15 毫秒
31.
Nadia Walsby Franciska Sundholm Tanja Kallio Gran Sundholm 《Journal of polymer science. Part A, Polymer chemistry》2001,39(17):3008-3017
A series of commercial fluoropolymer films was irradiated with an electron beam, grafted with styrene, and sulfonated. The influence of the initial fluoropolymer on the grafting yields and the properties of the grafted and sulfonated membranes were investigated. The same synthesis procedure can be followed for most fluoropolymers and samples with a similar degree of grafting, and a homogenous polystyrene distribution can be prepared by varying the absorbed dose. The main difference among different fluoropolymer‐based membranes is the water uptake from liquid water that has a roughly linear dependence on the crystallinity of the sample. The more amorphous the initial material, the greater the water uptake. Mechanical properties of the membranes at 50% relative humidity differ less than those of the starting materials and are comparable to those of Nafion® 105. © 2001 John Wiley & Sons, Inc. J Polym Sci Part A: Polym Chem 39: 3008–3017, 2001 相似文献
32.
Alja Prah Tanja Gavrani Andrej Perdih Marija Sollner Dolenc Janez Mavri 《Molecules (Basel, Switzerland)》2022,27(19)
Monoamine oxidases (MAOs) are an important group of enzymes involved in the degradation of neurotransmitters and their imbalanced mode of action may lead to the development of various neuropsychiatric or neurodegenerative disorders. In this work, we report the results of an in-depth computational study in which we performed a static and a dynamic analysis of a series of substituted β-carboline natural products, found mainly in roasted coffee and tobacco smoke, that bind to the active site of the MAO-A isoform. By applying molecular docking in conjunction with structure-based pharmacophores and molecular dynamics simulations coupled with dynamic pharmacophores, we extensively investigated the geometric aspects of MAO-A binding. To gain insight into the energetics of binding, we used the linear interaction energy (LIE) method and determined the key anchors that allow productive β-carboline binding to MAO-A. The results presented herein could be applied in the rational structure-based design and optimization of β-carbolines towards preclinical candidates that would target the MAO-A enzyme and would be applicable especially in the treatment of mental disorders such as depression. 相似文献
33.
34.
Flavin Adenine Dinucleotide and N5,N10‐Methenyltetrahydrofolate are the in planta Cofactors of Arabidopsis thaliana Cryptochrome 3 下载免费PDF全文
Tanja Göbel Stefan Reisbacher Alfred Batschauer Richard Pokorny 《Photochemistry and photobiology》2017,93(1):355-362
Members of the cryptochrome/photolyase family (CPF) of proteins utilize noncovalently bound light‐absorbing cofactors for their biological function. Usually, the identity of these cofactors is determined after expression in heterologous systems leaving the question unanswered whether these cofactors are identical to the indigenous ones. Here, cryptochrome 3 from Arabidopsis thaliana was expressed as a fusion with the green fluorescent protein in Arabidopsis plants. Besides the confirmation of the earlier report of its localization in chloroplasts, our data indicate that fractions of the fusion protein are present in the stroma and associated with thylakoids, respectively. Furthermore, it is shown that the fusion protein expressed in planta contains the same cofactors as the His6‐tagged protein expressed in Escherichia coli, that is, flavin adenine dinucleotide and N5,N10‐methenyltetrahydrofolate. This demonstrates that the heterologously expressed cryptochrome 3, characterized in a number of previous studies, is a valid surrogate of the corresponding protein expressed in plants. To our knowledge, this is also a first conclusive analysis of cofactors bound to an Arabidopsis protein belonging to the CPF and purified from plant tissue. 相似文献
35.
Site‐Selective Disulfide Modification of Proteins: Expanding Diversity beyond the Proteome 下载免费PDF全文
Dr. Seah Ling Kuan Dr. Tao Wang Prof. Dr. Tanja Weil 《Chemistry (Weinheim an der Bergstrasse, Germany)》2016,22(48):17112-17129
The synthetic transformation of polypeptides with molecular accuracy holds great promise for providing functional and structural diversity beyond the proteome. Consequently, the last decade has seen an exponential growth of site‐directed chemistry to install additional features into peptides and proteins even inside living cells. The disulfide rebridging strategy has emerged as a powerful tool for site‐selective modifications since most proteins contain disulfide bonds. In this Review, we present the chemical design, advantages and limitations of the disulfide rebridging reagents, while summarizing their relevance for synthetic customization of functional protein bioconjugates, as well as the resultant impact and advancement for biomedical applications. 相似文献
36.
37.
Andreas J. Steiner Georg Schitter Arnold E. Stütz Tanja M. Wrodnigg Chris A. Tarling Stephen G. Withers Don J. Mahuran Michael B. Tropak 《Tetrahedron: Asymmetry》2009,20(6-8):832-835
Cyclisation by double reductive amination of 2-acetamino-2-deoxy-d-xylo-hexos-5-ulose with N-2 protected l-lysine derivatives provided 2-acetamino-1,2-dideoxynojirimycin derivatives without any observable epimer formation at C-5. Modifications on the lysine moiety gave access to lipophilic derivatives that exhibited improved hexosaminidase inhibitory activities. 相似文献
38.
39.
Michael Müller Prof. Dr. Markus Albrecht Verena Gossen Tanja Peters Andreas Hoffmann Prof. Dr. Gerhard Raabe Dr. Arto Valkonen Prof. Kari Rissanen 《Chemistry (Weinheim an der Bergstrasse, Germany)》2010,16(41):12446-12453
The directionality of interaction of electron‐deficient π systems with spherical anions (e.g,. halides) can be controlled by secondary effects like NH or CH hydrogen bonding. In this study a series of pentafluorophenyl‐substituted salts with polyhalide anions is investigated. The compounds are obtained by aerobic oxidation of the corresponding halide upon crystallization. Solid‐state structures reveal that in bromide 2 , directing NH–anion interactions position the bromide ion in an η1‐type fashion over but not in the center of the aromatic ring. The same directing forces are effective in corresponding tribromide salt 3 . In the crystal, the bromide ion is paneled by four electron‐deficient aromatic ring systems. In addition, compounds 4 and 6 , which have triiodide and the rare tetraiodide dianion as anions, are described. Computational studies reveal that the latter is highly unstable. In the present case it is stabilized by the crystal lattice, for example, by interaction with electron‐deficient π systems. 相似文献
40.
Valeri I. Poltev Victor M. Anisimov Victor I. Danilov Tanja van Mourik Alexandra Deriabina Eduardo González Maria Padua Dolores Garcia Francisco Rivas Nina Polteva 《International journal of quantum chemistry》2010,110(13):2548-2559
We apply DFT calculations to deoxydinucleoside monophosphates (dDMPs) which represent minimal fragments of the DNA chain to study the molecular basis of stability of the DNA duplex, the origin of its polymorphism and conformational heterogeneity. In this work, we continue our previous studies of dDMPs where we detected internal energy minima corresponding to the “classical” B conformation (BI‐form), which is the dominant form in the crystals of oligonucleotide duplexes. We obtained BI local energy minima for all existing base sequences of dDMPs. In the present study, we extend our analysis to other families of DNA conformations, successfully identifying A, BI, and BII energy minima for all dDMP sequences. These conformations demonstrate distinct differences in sugar ring puckering, but similar sequence‐dependent base arrangements. Internal energies of BI and BII conformers are close to each other for nearly all the base sequences. The dGpdG, dTpdG, and dCpdA dDMPs slightly favor the BII conformation, which agrees with these sequences being more frequently experimentally encountered in the BII form. We have found BII‐like structures of dDMPs for the base sequences both existing in crystals in BII conformation and those not yet encountered in crystals till now. On the other hand, we failed to obtain dDMP energy minima corresponding to the Z family of DNA conformations, thus giving us the ground to conclude that these conformations are stabilized in both crystals and solutions by external factors, presumably by interactions with various components of the media. Overall the accumulated computational data demonstrate that the A, BI, and BII families of DNA conformations originate from the corresponding local energy minimum conformations of dDMPs, thus determining structural stability of a single DNA strand during the processes of unwinding and rewinding of DNA. © 2010 Wiley Periodicals, Inc. Int J Quantum Chem 110:2548–2559, 2010 相似文献