排序方式: 共有79条查询结果,搜索用时 15 毫秒
21.
Kan T Hosokawa S Nara S Tamiya H Shirahama H Matsuda F 《The Journal of organic chemistry》2004,69(25):8956-8958
Complete reversal of diastereoselectivity was observed in the SmI(2)-promoted ketyl-olefin coupling cyclizations of the hydroxy ketone or aldehyde and its acetate. For example, the stereodivergent synthesis of the epimeric five-membered-ring alcohols 2 and 4 has been accomplished through the SmI(2)-induced ketyl-olefin coupling cyclizations of the delta-hydroxy ketone 1 and delta-acetoxy ketone 3. 相似文献
22.
Aya Hashimoto Liang‐da Chiu Keigo Sawada Tomohiko Ikeuchi Katsumasa Fujita Masahide Takedachi Yoshinori Yamaguchi Satoshi Kawata Shinya Murakami Eiichi Tamiya 《Journal of Raman spectroscopy : JRS》2014,45(2):157-161
Hydroxyapatite (HA) is synthesized at early stages of bone formation by osteoblasts. Nondestructive observation of early stages of osteoblastic mineralization provides crucial information for biological mechanism of bone formation. Raman microscopy serves as an ideal tool to observe the osteoblastic mineralization process because it shows the chemical information of the sample at a minimally invasive level. In addition, HA is a marker for osteoblastic mineralization, and HA Raman signal is strong enough to identify mineralized spots in osteoblasts. In this research, we visualized the distribution of HA in cultured mouse osteoblasts by Raman imaging and observed the location of the mineralized spots in the culture. We monitored HA Raman signal from osteoblast culture for 3 days after administrating the osteogenic differentiation medium and observed Raman signal associated with HA. We identified mineralized spots of KUSA‐A1 by Raman imaging constructed from the distribution of HA Raman signal. We successfully visualized the distribution of the mineralized spots in the culture of KUSA‐A1. We compared our Raman images with Alizarin red S staining assay, which was a conventional method to evaluate the mineralization process. Raman imaging of the KUSA‐A1 culture visualized the mineralized spots more accurately than Alizarin red S staining assay. Raman imaging of HA serves as a powerful tool to identify the mineralized spots in an in vitro culture of osteogenic lineage cells. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
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24.
Koji Kano Yoko Tamiya Chuhei Otsuki Takako Shimomura Teruhisa Ohno Osamu Hayashida 《Supramolecular chemistry》2013,25(2-3):137-143
Abstract Chiral recognition of binaphthyl derivatives, such as 1,1′-bi-2-naphthol (1), 1,1′-binaphthyl-2,2′-diyl hydrogen phosphate (2), and 2,2′-dihydroxy-1,1′-binaphthyl-3,3′-dicarboxylic acid (3), by cyclodextrins (CDxs) has been studied. The S enantiomers of 1 and 2 are bound to heptakis(2,3,6-tri-O-methyl)-β-CDx (TMe-β-CDx) as well as β-CDx more strongly than the R enantiomers. The molecular mechanics and molecular dynamics calculations for the 1:1 complex of 1 and β-CDx suggest that more effective van der Waals contacts and intermolecular hydrogen bonding stabilize the complex of S-1 compared with that of R-1. Meanwhile the R enantiomer of 3 is the preferable guest for β- and TMe-β-CDxs. Circular dichroism spectroscopy suggests that the complex of S-3 is more unstable than that of R-3 because the dihedral angle of the naphthalene planes of S-3 needs to be reduced for forming the inclusion complex. The enantiomers of the guest binaphthyls are completely separated by means of capillary zone electrophoresis (CZE) when TMe-β-CDx is used as a separating agent. The results of the CZE correspond well with those of the binding constants of the inclusion complexes. 相似文献
25.
Electroactive chitosan nanoparticles for the detection of single-nucleotide polymorphisms using peptide nucleic acids 总被引:1,自引:0,他引:1
Here we report an electrochemical biosensor that would allow for simple and rapid analysis of nucleic acids in combination
with nuclease activity on nucleic acids and electroactive bionanoparticles. The detection of single-nucleotide polymorphisms
(SNPs) using PNA probes takes advantage of the significant structural and physicochemical differences between the full hybrids
and SNPs in PNA/DNA and DNA/DNA duplexes. Ferrocene-conjugated chitosan nanoparticles (Chi-Fc) were used as the electroactive
indicator of hybridization. Chi-Fc had no affinity towards the neutral PNA probe immobilized on a gold electrode (AuE) surface.
When the PNA probe on the electrode surface hybridized with a full-complementary target DNA, Chi-Fc electrostatically attached
to the negatively-charged phosphate backbone of DNA on the surface and gave rise to a high electrochemical oxidation signal
from ferrocene at ∼0.30 V. Exposing the surface to a single-stranded DNA specific nuclease, Nuclease S1, was found to be very
effective for removing the nonspecifically adsorbed SNP DNA. An SNP in the target DNA to PNA made it susceptible to the enzymatic
digestion. After the enzymatic digestion and subsequent exposure to Chi-Fc, the presence of SNPs was determined by monitoring
the changes in the electrical current response of Chi-Fc. The method provided a detection limit of 1 fM (S/N = 3) for the
target DNA oligonucleotide. Additionally, asymmetric PCR was employed to detect the presence of genetically modified organism
(GMO) in standard Roundup Ready soybean samples. PNA-mediated PCR amplification of real DNA samples was performed to detect
SNPs related to alcolohol dehydrogenase (ALDH). Chitosan nanoparticles are promising biometarials for various analytical and
pharmaceutical applications.
Figure The electrochemical method for SNP detection using PNA probes and chitosan nanoparticles takes advantage of the significant
structural and physicochemical differences between PNA/DNA and DNA/DNA duplexes. Single-stranded DNA specific enzymes selectively
choose these SNP sites and hydrolyze the DNA molecules on gold electrode (AuE) surface.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
26.
Tamiya M Ohmori K Kitamura M Kato H Arai T Oorui M Suzuki K 《Chemistry (Weinheim an der Bergstrasse, Germany)》2007,13(35):9791-9823
A general approach to the regio- and stereoselective total synthesis of the benanomicin-pradimicin antibiotics (BpAs) is described. Construction of the aglycon has been achieved by 1) the diastereoselective ring-opening of a biaryl lactone by using (R)-valinol as a chiral nucleophile and 2) the stereocontrolled semi-pinacol cyclization of the aldehyde acetal by using SmI(2) in the presence of BF(3)OEt(2) and a proton source to afford the ABCD tetracyclic monoprotected diol. This strategy enabled us to control the two stereogenic sites in the B ring (C-5 and C-6) and the regioselective introduction of the carbohydrate moiety. The ABCD tetracycle could serve as an ideal platform for the divergent access to various BpAs. The amino acid (D-alanine) was introduced onto the ABCD tetracycle. Glycosylation was promoted by the combination of Cp(2)HfCl(2) and AgOTf (1:2 ratio). Construction of the E ring followed by deprotection completed the first total synthesis of benanomicin A (2 a), benanomicin B (2 b), and pradimicin A (1 a). The route is flexible enough to allow the synthesis of other congeners differing in their amino acid and carbohydrate moieties. 相似文献
27.
Tamiya N Yagi T 《Proceedings of the Japan Academy. Series B, Physical and biological sciences》2011,87(3):41-52
Erabutoxins a and b are neurotoxins isolated from venom of a sea snake Laticauda semifasciata (erabu-umihebi). Amino acid sequences of the toxins indicated that the toxins are members of a superfamily consisting of short and long neurotoxins and cytotoxins found in sea snakes and terrestrial snakes. The short neurotoxins to which erabutoxins belong act by blocking the nicotinic acetylcholine receptor on the post synaptic membrane in a manner similar to that of curare. X-ray crystallography and NMR analyses showed that the toxins have a three-finger structure, in which three fingers made of three loops emerging from a dense core make a gently concave surface of the protein. The sequence comparison and the location of essential residues on the protein suggested the mechanism of binding of the toxin to the acetylcholine receptor. Classification of snakes by means of sequence comparison and that based on different morphological features were inconsistent, which led the authors to propose a hypothesis "Evolution without divergence." 相似文献
28.
Sugano Y Vestergaard MC Saito M Tamiya E 《Chemical communications (Cambridge, England)》2011,47(25):7176-7178
A unique artificial catalyst that mimics the structure of active sites in real enzymes using functionalized carbon nanotubes is presented. This concept will allow for the potential construction of a library of biomimetic catalysts for enzyme active centers, for which the structure-catalysis relationships are well defined. 相似文献
29.
Nagatani N Yamanaka K Ushijima H Koketsu R Sasaki T Ikuta K Saito M Miyahara T Tamiya E 《The Analyst》2012,137(15):3422-3426
Influenza virus RNA was amplified by a continuous-flow polydimethylsiloxane microfluidic RT-PCR chip within 15-20 min. The amplified influenza virus RNA was observed with the naked eye, as the red color at the test line, using a lateral flow immunoassay within 1 min. 相似文献
30.
The venom of the sea krait, Laticauda semifasciata, consists primarily of two toxic proteins, phospholipase A2 (PLA2) and a three-finger-structure toxin. We have cloned both toxic protein genes, including the upstream region. PLA2 genes contain three types of inserted sequences: an AG-rich region, a chicken repeat 1-like long interspersed nucleotide element sequence and an intron II 3′ side repeat sequence. The molecular divergence of L. semifasciata PLA2 genes was defined on the basis of the inserted sequences and their sequence homology. The length of intron I in the three-finger-structure toxin genes differs from species to species. The alignment analysis of intron I of the three-finger-structure toxin genes revealed that the intron I sequence of the ancestral gene comprised ten genetic regions. A hypothetical evolutionary process for the three-finger-structure toxin genes has also been developed. 相似文献