全文获取类型
收费全文 | 90篇 |
免费 | 3篇 |
专业分类
化学 | 53篇 |
数学 | 8篇 |
物理学 | 32篇 |
出版年
2022年 | 2篇 |
2020年 | 1篇 |
2015年 | 1篇 |
2013年 | 4篇 |
2012年 | 3篇 |
2011年 | 1篇 |
2009年 | 2篇 |
2008年 | 5篇 |
2007年 | 4篇 |
2006年 | 5篇 |
2005年 | 4篇 |
2004年 | 5篇 |
2003年 | 3篇 |
2002年 | 8篇 |
2001年 | 3篇 |
2000年 | 3篇 |
1994年 | 2篇 |
1993年 | 4篇 |
1992年 | 6篇 |
1991年 | 1篇 |
1990年 | 8篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1987年 | 3篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1975年 | 1篇 |
排序方式: 共有93条查询结果,搜索用时 15 毫秒
1.
Talukder MM Takeyama T Hayashi Y Wu JC Kawanishi T Shimizu N Ogino C 《Applied biochemistry and biotechnology》2003,110(2):101-112
The activity and stability of Chromobacterium viscosum lipase (glycerolester hydrolase, EC 3.1.1.3)-catalyzed olive oil hydrolysis in sodium bis (2-ethyl-1-hexyl)sulfosuccinate (AOT)/isooctane reverse micelles is increased appreciably when low molecular weight polyethylene
glycol (PEG 400) is added to the reverse micelles. To understand the effect of PEG 400 on the phase behavior of the reverse
micellar system, the phase diagram of AOT/PEG 400/water/isooctane system was studied. The influences of relevant parameters
on the catalytic activity in AOT/PEG 400 reverse micelles were investigated and compared with the results in the simple AOT
reverse micelles. In the presence of PEG 400, the linear decreasing trend of the lipase activity with AOT concentration, which
is observed in the simple AOT reverse micelles, disappeared. Enzyme entrapped in AOT/PEG reverse micelles was very stable,
retaining>75% of its initial activity after 60 d, whereas the half-life in simple AOT reverse micelles was 38 d. The kinetics
parameter maximum velocity (V
max)exhibiting the temperature dependence and the activation energy obtained by Arrhenius plot was suppressed significantly by
the addition of PEG 400. 相似文献
2.
3.
Hidenobu Takeyama Masatoshi Kobayashi Hirofumi Yajima Ryuichi Endo Kaoru Kohyama Katsuyoshi Nishinari 《Macromolecular Symposia》1993,76(1):83-88
Amylose gel is obtained by cooling amylose aqueous solution at a certain cooling rate. In order to clarify the gelation process of amylose in the mixed solvent of water and DMSO, the characteristics of turbidity and dynamic viscoelasticity of the solution as a function of temperature have been studied by optical and rheological methods, respectively. Accordingly, cloud temperature (Tc) at which the aggregation of amylose occurs, and gelation temperature (Tgel) at which the elasticity began to appear were obtained. Tc and Tgel were strongly dependent on cooling rate, and these values shifted to higher temperatures with decreasing cooling rate. However, there was difference between Tc and Tgel, and Tc was higher than Tgel, indicating the gelation process occurs in two stages. 相似文献
4.
We consider the q-hypergeometric equation with q
N = 1 and , , . We solve this equation on the space of functions given by a power series multiplied by a power of the logarithmic function. We prove that the subspace of solutions is two-dimensional over the field of quasi-constants. We get a basis for this space explicitly. In terms of this basis, we represent the q-hypergeometric function of the Barnes type constructed by Nishizawa and Ueno. Then we see that this function has logarithmic singularity at the origin. This is a difference between the q-hypergeometric functions with 0 < |q| < 1 and at |q| = 1. 相似文献
5.
Determination of overt carnitine palmitoyltransferase by reversed-phase high-performance liquid chromatography 总被引:1,自引:0,他引:1
A method for the determination of carnitine palmitoyltransferase I (CPT I; EC 2.3.1.19) in isolated rat liver mitochondria by reversed-phase high-performance liquid chromatography is described. Enzyme activity is assayed by direct determination of coenzyme A (CoA) released from palmitoyl-CoA within 60 min by a linear gradient system. CPT 1 in rat liver mitochondria can be assayed from only 30 micrograms of mitochondrial protein per millilitre of assay mixture. The changes in the kinetic parameters of CPT I, including Ki for malonyl-CoA, resulting from the fasting-feeding cycle are also discussed. 相似文献
6.
Enzyme immunoassay of somatostatin (SS)-like immunoreactive substance in bovine milk 总被引:1,自引:0,他引:1
M Takeyama N Yanaga K Yarimizu J Ono R Takaki N Fujii H Yajima 《Chemical & pharmaceutical bulletin》1990,38(2):456-459
A sensitive and specific enzyme immunoassay (EIA) for somatostatin (SS)-like immunoreactivity (SS-LI) was developed with the use of beta-D-galactosidase labeled antigen. The minimum amount of SS-like immunoreactive substance (SS-IS) detectable by this method was 1.0 fmol/well (25 pmol/l). The level of SS-IS in bovine foremilk was about 20 pmol/l, and the level was unchanged after delivery. On the other hand, the levels of gastrin releasing peptide (GRP)-IS and vasoactive intestinal polypeptide (VIP)-IS in bovine foremilk were very high, but fell during 1 week after delivery to about 10% of those in foremilk. 相似文献
7.
A sensitive and specific double-antibody enzyme immunoassay (EIA) for a thyrotropin releasing hormone (TRH)-like immunoreactive substance has been developed. In order to synthesize TRH-labeled beta-D-galactosidase (beta-gal), a newly devised TRH derivative, pGlu-His-Pro-NH-(CH2)6-NH2 (TRH-Hex), was employed. TRH-Hex was linked to beta-gal by the N-(epsilon-maleimidocaproyloxy) succinimide coupling procedure. For competitive reactions, the TRH antibody was incubated with standard TRH and TRH-Hex-beta-gal (delayed addition). Free and antibody-bound enzyme hapten were separated by using an anti-rabbit immunoglobulin G coated immunoplate. Activity of the enzyme on the plate was fluorometrically determined. The present immunoassay allows detection of 0.8 to 100 pmol/well of TRH. 相似文献
8.
T. Minamisono Y. Nojiri K. Matsuta K. Takeyama A. Kitagawa T. Ohtsubo A. Ozawa 《Hyperfine Interactions》1990,59(1-4):31-40
Electromagnetic moments of doubly closed shell ±1 nucleon nuclei were studied. Recent technical developments of the β-NMR
was shown that was applicable for the polarized β emitters. Also, the implication of those new moments were discussed especially
on the nuclear interactions and non nucleonic degrees of freedom. 相似文献
9.
Arakaki Atsushi Takeyama Haruko Tanaka Tsuyoshi Matsunaga Tadashi 《Applied biochemistry and biotechnology》2002,98(1-9):833-840
Cadmium recovery by a sulfate-reducing magnetotactic bacterium, Desulfovibrio magneticus strain RS-1, was investigated. D. magneticus precipitated >95% of cadmium at an initial concentration of 1.3 ppm in the growth medium. Electron microscopic analysis revealed
that D. magneticus formed electron-dense particles on its surface when cultivated in the presence of cadmium ions (Cd2+). Sulfide was also found in the precipitate, and the composition ratio of sulfide/cadmium was 0.7. Sixty percent of viable
RS-1 cells was recovered by a simple magnetic separation revealing the removal of 58% cadmium from the culture medium. 相似文献
10.
Ryuiti Ikee Haruo Takeyama 《Journal of Quantitative Spectroscopy & Radiative Transfer》1975,15(6):543-547
The brush cathode helium discharge in the magnetic field has been operated stably at discharge currents larger than those without magnetic field. The diameter of the plasma column has been determined by the configuration of the magnetic field. The measurements of the spectral intensities of the recombination continuum followed by the 23S-n3P series reveals that the electron density is 1·8 × 1013 cm-3 and the electron temperature is 0·17 eV at a discharge current of 500 mA and a pressure of 0·9 torr for a magnetic flux density of 1·3 kG. The principal quantum number for line merging is 20. 相似文献