全文获取类型
收费全文 | 238篇 |
免费 | 8篇 |
专业分类
化学 | 191篇 |
晶体学 | 7篇 |
力学 | 6篇 |
数学 | 10篇 |
物理学 | 32篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 6篇 |
2020年 | 2篇 |
2019年 | 7篇 |
2018年 | 3篇 |
2017年 | 1篇 |
2016年 | 5篇 |
2015年 | 8篇 |
2014年 | 5篇 |
2013年 | 10篇 |
2012年 | 3篇 |
2011年 | 17篇 |
2010年 | 8篇 |
2009年 | 9篇 |
2008年 | 11篇 |
2007年 | 13篇 |
2006年 | 19篇 |
2005年 | 15篇 |
2004年 | 20篇 |
2003年 | 11篇 |
2002年 | 7篇 |
2001年 | 1篇 |
2000年 | 4篇 |
1999年 | 4篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1989年 | 2篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 3篇 |
1980年 | 7篇 |
1979年 | 3篇 |
1978年 | 5篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1973年 | 2篇 |
1972年 | 1篇 |
1970年 | 2篇 |
1968年 | 2篇 |
1966年 | 1篇 |
排序方式: 共有246条查询结果,搜索用时 15 毫秒
91.
Masuo Aizawa Aya Morioka Hideaki Matsuoka Shuichi Suzuki Yoichi Nagamura Rikio Shinohara Isao Ishiguro 《Applied biochemistry and biotechnology》1976,1(4):319-328
An enzyme immunosensor has been developed for assaying human immunoglobulin G (IgG). The sensor is composed of an oxygen sensoring system and an antibody-binding membrane. The assay procedure involves the competitive immunochemical reaction of the membrane-bound antibody with nonlabeled and catalase-labeled IgG and the electrochemical determination of membrane-bound catalase activity. The analytical result is directly displayed by the output current of the sensor. The sensor exhibited an excellent performance in monitoring specifically human IgG. 相似文献
92.
E Otsuka M Ubasawa S Morioka M Ikehara 《Journal of the American Chemical Society》1973,95(14):4725-4733
93.
Nishimura K Tsumagari H Morioka A Yamauchi Y Miyashita K Lu S Jisaka M Nagaya T Yokota K 《Applied biochemistry and biotechnology》2002,102(1-6):239-250
The arachidonate cascade includes the cyclooxygenase (COX) pathway to form prostanoids and the lipoxygenase (LOX) pathway
to generate several oxygenated fatty acids, collectively called eicosanoids. Eicosanoids are suggested to play a dual role
in regulating cell survival and apoptosis in various types of cells through an unknown mechanism. We found apoptosis in cultured
Madin-Darby canine kidney (MDCK) cells treated with 12-O-tetradecanoylphorbol β-acetate (TPA), a potent tumor promoter, and nordihydroguaiaretic acid (NDGA), a LOX inhibitor. The
effect of TPA was synergistically stimulated along with NDGA. Aspirin, a COX inhibitor, was not effective. The target of NDGA
might be different from the mechanism involving a LOX activity in some kinds of carcinoma cells because the increased expression
of 12-LOX was not detected in MDCK cells treated with TPA. Caspase and poly(ADP-ribose) metabolites were found to be involved
in the signal transduction pathway of the TPA- and NDGA-induced apoptosis in MDCK cells. Alternatively, hydrogen peroxide-induced
apoptosis was not affected by NDGA. Thus, the TPA-induced response involved the mechanism independent of the oxidative stress.
Obesity is a risk factor for severe diseases including noninsulin-dependent diabetes and atherosclerosis characterized by
the changes of cell properties of adipocytes. We found that conjugated linolenic acid from bitter gourd was able to induce
apoptosis in mouse preadipogenic 3T3-L1 cells. The findings provide the potential use of conjugated fatty acids to regulate
obesity. 相似文献
94.
95.
Simultaneous separation of nonionic surfactants and polyethylene glycols by reversed phase high performance liquid chromatography 总被引:2,自引:0,他引:2
Summary The simultaneous separation of polyethylene glycol and its derivatives such as the lauryl alcohol and lauric acid ethoxylate
oligomers was carried out by reversed phase high performance liquid chromatography. Branched fluorinated silica gel columns
combined with evaporative light scattering detection were used for the characterization of nonionic surfactants. Lauryl alcohol
ethoxylate oligomers were separated at 10°C with an isocratic eluent according to ethoxylate number and the retention time
of the oligomers decreases with increasing ethoxylate number. The Van’t Hoff plots of retention factor of lauryl alcohol ethoxylate
gave a complex cure, which is anomalous behavior for reversed phase high performance liquid chromatography. The anomalous
Van’t Hoff plots were explained by a partial conformational change from polar to less polar conformers with increasing temperature.
The most significant features for the analysis of the lauryl alcohol ethoxylate were the use of acetonitrile as mobile phase
and operating temperature. The polyethylene glycol was separated according to ethoxylate number and the retention time of
oligomers increased with increasing ethoxylate number. The Van’t Hoff plots of retention factor of polyethylene glycol had
negative slopes. It was presumed that the polar conformation of the ethylene oxide chain decreased with increasing temperature.
The lauryl alcohol ethoxylate and polyethylene glycol were separated simultaneously in gradient elution as a result of the
conformational change of the ethylene oxide chain. As a practical example, lauric acid ethoxylate simultaneously separated
into free polyethylene glycol, ethoxylate monolaurate and ethoxylate dilaurate in gradient elution. 相似文献
96.
We study the spectral properties of Schrödinger operators on perturbed lattices. We shall prove the non-existence or the discreteness of embedded eigenvalues, the limiting absorption principle for the resolvent, construct a spectral representation, and define the S-matrix. Our theory covers the square, triangular, diamond, Kagome lattices, as well as the ladder, the graphite and the subdivision of square lattice. 相似文献
97.
98.
Pressure wave propagation into a separated gas-liquid layer in a horizontal duct with a step is investigated analytically. The linear solution is derived assuming a large density ratio of liquid to gas. The solution can be found first for the gas layer and then for the liquid layer. The linear wave in a liquid layer is valid even for fairly large initial pressure ratios, and clearly exhibits the dispersive characteristics of the pressure wave in a liquid layer. As the initial pressure ratio is increased, the pressure wave in the gas layer becomes a shock wave. Thus, its effect on the wave in a liquid layer can be found analytically by modifying the boundary condition in part. The wave in a liquid layer consists of a main wave, which propagates with the shock speed in gas, and a precursor wave, whose front propagates with the speed of sound in liquid. The precursor wave has an oscillatory structure; its amplitude increases with increasing shock strength and also with liquid layer thickness. 相似文献
99.
Masahiro Yoshida Takako Suzuki Hideharu Ishida Makoto Kiso Akira Hasegawa 《Journal of carbohydrate chemistry》2013,32(2):147-162
Abstract Three sialyl-Lex epitope analogs, which carry fucose and α-sialyl-(2→3)-galactose residues at O-2 and O-3, O-3 and O-2, and O-4 and O-6 positions of 1-deoxy-D-glucose backbone, respectively, have been synthesized. Glycosylation of 1,5-anhydro-4,6-O-benzylidene-D-glucitol (1) or 1,5-anhydro-6-O-benzoyl-2,3-di-O-benzyl-d-glucitol (4) prepared from 1,5-anhydro-d-glucitol, with methyl 2,3,4-tri-O-benzyl-1-thio-β-L-fucopyranoside (5) using dimethyl(methylthio)sulfonium triflate (DMTST) as a promoter, afforded the corresponding fucosyl 1,5-anhydro-d-glucitol derivatives 7, 8 and 9. Glycosylation of 7, 8 or 10 derived from 9, with methyl O-(methyl 5-acetamido-4,7,8,9-tetra-O-acetyl-3,5-dideoxy-d-glyceroα-d-galacto-2-nonulopyranosylonate)-(2→3)-2,4,6-tri-O-benzoyl-1-thio-β-d-galactopyranoside (11) in the presence of DMTST gave the expected tetrasaccharide derivatives 12, 16 and 20. Hydrolysis of the benzylidene group in 12 and 16 gave compounds 13 and 17. Finally 13, 17 and 20 were transformed, by reductive removal of the benzyl groups, O-deacylation and subsequent hydrolysis of the methyl ester, into the sialyl-Lex epitope analogs 15, 19 and 22, respectively. 相似文献
100.
Koji Kano Yoko Tamiya Chuhei Otsuki Takako Shimomura Teruhisa Ohno Osamu Hayashida 《Supramolecular chemistry》2013,25(2-3):137-143
Abstract Chiral recognition of binaphthyl derivatives, such as 1,1′-bi-2-naphthol (1), 1,1′-binaphthyl-2,2′-diyl hydrogen phosphate (2), and 2,2′-dihydroxy-1,1′-binaphthyl-3,3′-dicarboxylic acid (3), by cyclodextrins (CDxs) has been studied. The S enantiomers of 1 and 2 are bound to heptakis(2,3,6-tri-O-methyl)-β-CDx (TMe-β-CDx) as well as β-CDx more strongly than the R enantiomers. The molecular mechanics and molecular dynamics calculations for the 1:1 complex of 1 and β-CDx suggest that more effective van der Waals contacts and intermolecular hydrogen bonding stabilize the complex of S-1 compared with that of R-1. Meanwhile the R enantiomer of 3 is the preferable guest for β- and TMe-β-CDxs. Circular dichroism spectroscopy suggests that the complex of S-3 is more unstable than that of R-3 because the dihedral angle of the naphthalene planes of S-3 needs to be reduced for forming the inclusion complex. The enantiomers of the guest binaphthyls are completely separated by means of capillary zone electrophoresis (CZE) when TMe-β-CDx is used as a separating agent. The results of the CZE correspond well with those of the binding constants of the inclusion complexes. 相似文献