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91.
The inverse mean first passage time problem is given a positive matrix M∈Rn,n, then when does there exist an n-state discrete-time homogeneous ergodic Markov chain C, whose mean first passage matrix is M? The inverse M-matrix problem is given a nonnegative matrix A, then when is A an inverse of an M-matrix. The main thrust of this paper is to show that the existence of a solution to one of the problems can be characterized by the existence of a solution to the other. In so doing we extend earlier results of Tetali and Fiedler. 相似文献
92.
Chen X Fung YM Chan WY Wong PS Yeung HS Chan TW 《Journal of the American Society for Mass Spectrometry》2011,22(12):2232-2245
Electron capture dissociation (ECD) of model peptides adducted with first row divalent transition metal ions, including Mn2+, Fe2+, Co2+, Ni2+, Cu2+, and Zn2+, were investigated. Model peptides with general sequence of ZGGGXGGGZ were used as probes to unveil the ECD mechanism of
metalated peptides, where X is either V or W; and Z is either R or N. Peptides metalated with different divalent transition
metal ions were found to generate different ECD tandem mass spectra. ECD spectra of peptides metalated by Mn2+ and Zn2+ were similar to those generated by ECD of peptides adducted with alkaline earth metal ions. Series of c-/z-type fragment ions with and without metal ions were observed. ECD of Fe2+, Co2+, and Ni2+ adducted peptides yielded abundant metalated a-/y-type fragment ions; whereas ECD of Cu2+ adducted peptides generated predominantly metalated b-/y-type fragment ions. From the present experimental results, it was postulated that electronic configuration of metal ions
is an important factor in determining the ECD behavior of the metalated peptides. Due presumably to the stability of the electronic
configuration, metal ions with fully-filled (i.e., Zn2+) and half filled (i.e., Mn2+) d-orbitals might not capture the incoming electron. Dissociation of the metal ions adducted peptides would proceed through
the usual ECD channel(s) via “hot-hydrogen” or “superbase” intermediates, to form series of c-/z
•- fragments. For other transition metal ions studied, reduction of the metal ions might occur preferentially. The energy liberated
by the metal ion reduction would provide enough internal energy to generate the “slow-heating” type of fragment ions, i.e.,
metalated a-/y- fragments and metalated b-/y- fragments. 相似文献
93.
Chen X Chan WY Wong PS Yeung HS Chan TW 《Journal of the American Society for Mass Spectrometry》2011,22(2):233-244
Peptides adducted with different divalent Group IIB metal ions (Zn2+, Cd2+, and Hg2+) were found to give very different ECD mass spectra. ECD of Zn2+ adducted peptides gave series of c-/z-type fragment ions with and without metal ions. ECD of Cd2+ and Hg2+ adducted model peptides gave mostly a-type fragment ions with M+• and fragment ions corresponding to losses of neutral side chain from M+•. No detectable a-ions could be observed in ECD spectra of Zn2+ adducted peptides. We rationalized the present findings by invoking both proton-electron recombination and metal-ion reduction
processes. As previously postulated, divalent metal-ions adducted peptides could adopt several forms, including (a) [M + Cat]2+, (b) [(M + Cat – H) + H]2+, and (c) [(M + Cat – 2H) + 2H]2+. The relative population of these precursor ions depends largely on the acidity of the metal–ion peptide complexes. Peptides
adducted with divalent metal-ions of small ionic radii (i.e., Zn2+) would form predominantly species (b) and (c); whereas peptides adducted with metal ions of larger ionic radii (i.e., Hg2+) would adopt predominantly species (a). Species (b) and (c) are believed to be essential for proton-electron recombination
process to give c-/z-type fragments via the labile ketylamino radical intermediates. Species (c) is particularly important for the formation of
non-metalated c-/z-type fragments. Without any mobile protons, species (a) are believed to undergo metal ion reduction and subsequently induce
spontaneous electron transfer from the peptide moiety to the charge-reduced metal ions. Depending on the exothermicity of
the electron transfer reaction, the peptide radical cations might be formed with substantial internal energy and might undergo
further dissociation to give structural related fragment ions. 相似文献
94.
A clickable, affinity-based probe (AfBP), which was modified from staurosporine (a natural product kinase inhibitor), has been synthesized and used in situ for activity-based proteome profiling of potential cellular targets of staurosporine in HepG2 cancer cells. 相似文献
95.
Ryu G Huang J Hofmann O Walshe CA Sze JY McClean GD Mosley A Rattle SJ deMello JC deMello AJ Bradley DD 《Lab on a chip》2011,11(9):1664-1670
We demonstrate a compact, low cost and practical fluorescence detection system for lab-on-a-chip applications. The system comprises a commercially available InGaN light emitting diode (501 nm) as light source, an organic or silicon photodiode detector, absorptive dye coated colour filters and linear and reflective polarisers. An injection moulded polystyrene microfluidic chip is used as the platform for fluorescence immunoassays for cardiac markers myoglobin and CK-MB. The optical limit of detection (LOD) is measured using a TransFluoSphere? suspension at 5.6 × 10(4) beads μl(-1) which can be equated to ~3 nM fluorescein equivalent concentration. The LOD for the human plasma immunoassays is measured as 1.5 ng ml(-1) for both myoglobin and CK-MB. 相似文献
96.
Li Qun Xu Bin Zhang Yu Chen Koon‐Gee Neoh En‐Tang Kang Guo Dong Fu 《Macromolecular rapid communications》2013,34(3):234-238
Graphene oxide (GO) nanosheets can be functionalized with reactive pentafluorophenyl ester via esterification of the carboxylic groups. The resulting reactive GO nanosheets provide a versatile platform for grafting of amino‐containing polymers or biomolecules via ester–amine coupling. Coupling of poly[(9,9‐dioctylfluorene)‐alt‐(4‐amino‐phenylcarbazole)] (PFCz‐NH2), amino‐terminated hyperbranched polyglycerol (HPG‐NH2), and lysozyme (Lyz) was illustrated. The Al/GO‐g‐PFCz/ITO sandwich thin‐film device exhibits bistable electrical switching and rewritable memory effects. The GO‐g‐Lyz nanohybrids exhibit high bactericidal efficacy against S. aureus and E. coli, while the GO‐g‐HPG nanohybrids exhibit reduced cytotoxicity toward 3T3 fibroblasts. 相似文献
97.
98.
Colorectal cancer (CRC) is the fourth most common cause of death from cancer in the world. The limitations of the currently available methods and biomarkers for CRC management highlight the necessity of finding novel markers. Metabonomics can be used to search for potential markers that can provide molecular insight into human CRC. The emergence of two-dimensional gas chromatography time of flight mass spectrometry (GC?×?GC/TOFMS) has comprehensively enhanced the metabolic space coverage of conventional GC/MS. In this study, a GC?×?GC/TOFMS was developed for the tissue-based global metabonomic profiling of CRC. A Pegasus GC?×?GC/TOFMS (Leco Corp., St. Joseph, MI, USA) system comprising an Agilent 7890 GC and Pegasus IV TOFMS was used for this purpose. An Agilent DB-1 (30 m?×?250 μm?×?0.25 μm) fused silica capillary column and a Restek Rxi®-17 (1 m?×?100 μm?×?0.10 μm) fused silica capillary column were used as the primary and secondary columns, respectively. The method was applied for global metabonomic profiling of matched CRC and normal tissues (n?=?63) obtained from 31 CRC patients during surgery. An attempt was also made to compare GC?×?GC/TOFMS with GC/MS and NMR in similar application. The results showed that the metabotype associated with CRC is distinct from that of normal tissue and led to the identification of chemically diverse marker metabolites. Metabolic pathway mapping suggested deregulation of various biochemical processes such as glycolysis, Krebs cycle, osmoregulation, steroid biosynthesis, eicosanoid biosynthesis, bile acid biosynthesis, lipid, amino acid and nucleotide metabolism. 相似文献
99.
100.
Wahl EH Fidric B Rella CW Koulikov S Kharlamov B Tan S Kachanov AA Richman BA Crosson ER Paldus BA Kalaskar S Bowling DR 《Isotopes in environmental and health studies》2006,42(1):21-35
Recent measurements of carbon isotopes in carbon dioxide using near-infrared, diode-laser-based cavity ring-down spectroscopy (CRDS) are presented. The CRDS system achieved good precision, often better than 0.2 per thousand, for 4% CO2 concentrations, and also achieved 0.15-0.25 per thousand precision in a 78 min measurement time with cryotrap-based pre-concentration of ambient CO2 concentrations (360 ppmv). These results were obtained with a CRDS system possessing a data rate of 40 ring-downs per second and a loss measurement of 4.0 x 10(-11) cm(-1) Hz(-1/2). Subsequently, the measurement time has been reduced to under 10 min. This standard of performance would enable a variety of high concentration (3-10%) isotopic measurements, such as medical human breath analysis or animal breath experiments. The extension of this ring-down to the 2 microm region would enable isotopic analysis at ambient concentrations, which, combined with the small size, robust design, and potential for frequent measurements at a remote site, make CRDS technology attractive for remote atmospheric measurement applications. 相似文献