Surface properties of "jellyfish": Langmuir monolayer and Langmuir-Blodgett film studies of recombinant aequorin

In this paper, we studied the surface properties of recombinant aequorin at the air-water interface. Using the Langmuir monolayer technique, the surface properties of aequorin were studied, including the surface pressure and surface potential-area isotherms, compression-decompression cycles, and stability on Trizma Base (Tris/HCl) buffer at pH 7.6. The results showed that aequorin formed a stable Langmuir monolayer and the surface pressure-area isotherms were dependent on both pH and ionic strength. At a pH higher or lower than 7.6, the limiting molecular area decreased. The circular dichroism (CD) spectra of aequorin in aqueous solutions explained this result: when the pH was higher than 7.6, the alpha-helix conformation changed to unordered structures, whereas at a pH lower than 7.6, the alpha-helix conformation changed to beta-sheet. The addition of calcium chloride to the Tris/HCl buffer subphase (pH 7.6) caused an increase of the limiting molecular area of the aequorin Langmuir monolayer. The fluorescence spectra of a Langmuir-Blodgett (LB) film of aequorin in the presence of calcium chloride indicated that the aequorin transformed to the apoaequorin.     

Double Yielding in a Styrene/Butadiene Star Block Copolymer

Summary: The morphology and tensile deformation behaviour of a highly asymmetric styrene/butadiene star block copolymer (polystyrene (PS) content = 74%) containing random PS‐co‐PB (polybutadiene) copolymer as a rubbery phase were investigated. The existence of double yielding, similar to that observed in some semicrystalline polymers, was detected in this nanostructured amorphous polymer. The observed phenomenon may be correlated with two different micromechanical processes taking place at the initial stage of deformation.

The stress‐strain curve of the star block copolymer prepared here (each curve represents a different method). The two yield points are clearly visible (labelled I and II).     

Effect Algebras with the Riesz Decomposition Property and AF C*-Algebras

Relations between effect algebras with Riesz decomposition properties and AF C^*-algebras are studied. The well-known one-one correspondence between countable MV-algebras and unital AF C^*-algebras whose Murray-von Neumann order is a lattice is extended to any unital AF C^* algebras and some more general effect algebras having the Riesz decomposition property. One-one correspondence between tracial states on AF C^*-algebras and states on the corresponding effect algebras is proved. In particular, pure (faithful) tracial states correspond to extremal (faithful) states on corresponding effect algebras.     

Sense and non-sense about polymers

This paper describes the present developments and future opportunities for industrial biopolymer applications.     

Occurrence of Cationic Intermediates and Deficient Control during the Enzymatic Cyclization of Squalene to Hopanoids

Tetracyclic triterpenes (for example, 3 ) are formed as minor products of the cyclization of squalene ( 1 ) by squalene/hopene cyclase; they have also been found in vivo. The identification of these side products offers new insight into the complex biosynthesis of the hopane skeleton, and proves that the enzyme has no absolute control of the conformation of the acyclic substrate squalene and that tetra- and pentacyclic carbocationic intermediates are involved (for example, 2 ) in the cyclization process.     

Spin Factors as Generalized Hermitian Algebras

We relate so-called spin factors and generalized Hermitian (GH-) algebras, both of which are partially ordered special Jordan algebras. Our main theorem states that positive-definite spin factors of dimension greater than one are mathematically equivalent to generalized Hermitian algebras of rank two. S. Pulmannová was supported by Research and Development Support Agency under the contract No. APVV-0071-06, grant VEGA 2/0032/09 and Center of Excellence SAS, CEPI I/2/2005.