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101.
THE PHOTOTOXICITY OF PHENYLHEPTATRIYNE: OXYGEN-DEPENDENT HEMOLYSIS OF HUMAN ERYTHROCYTES AND INACTIVATION OF Escherichia coli 总被引:3,自引:0,他引:3
Hai-Hua Gong Jacques Kagan Rosemarie Seitz Allison B. Stokes Frederick A. Meyer R. W. Tuveson 《Photochemistry and photobiology》1988,47(1):55-63
Abstract— Phenylheptatriyne (PHT) plus near-ultraviolet light(320–400 nm; NUV) hemolyzed human erythrocytes in an oxygen dependent manner. When the phototoxicity of PHT plus NUV was tested with a series of Escherichia coli strains carrying all four possible combinations of genes controlling excision proficiency ( uvrA6 vs uvrA + ) and catalase activity (HPII, katF vs katF *), the membrane was found to be an important lethal target. Consistent with this observation. PHT plus NUV did not induce histidine independent ( his-4 + ) mutations in the four tester strains (RT7h-RT10h). Using tester strain RT10h, it was shown that there was no inactivation by PHT plus NUV in nitrogen. Results of experiments with an E. coli fatty acid auxotroph (K1060) treated with PHT plus NUV are also consistent with membrane proteins being the chief targets for attack. Radicals were formed during the photolysis of PHT plus NUV in aqueous solutions, both in the presence of air and under nitrogen. Since PHT plus NUV did not hemolyze erythrocytes or inactivate E. coli cells under nitrogen, these radicals are not cytotoxic. 相似文献
102.
R Thedford L P Fauntleroy Y L Murray M Stokes C L Thornton 《Journal of chromatography. A》1992,582(1-2):253-257
High-performance liquid chromatography was used to analyze cell lysates and growth medium of mouse hepatoma cells, separately treated with N6-cyclopropyl-, N6-cyclobutyl-, and N6-cyclopentyladenosines, in an effort to gain insight into the mechanism by which these modified nucleosides exert their cytotoxic effect(s). The corresponding 5'-monophosphate of the respective modified nucleoside was detected in the separate cell lysate samples. Both the modified nucleoside and its corresponding 5'-monophosphate were detected in the separate growth medium samples and their relative concentrations therein were determined. These results indicate that the cytotoxicity of these N6-cycloalkylated nucleosides may be attributed to their 5'-monophosphates within the cells. 相似文献
103.
Solvation equilibria in very concentrated electrolyte solutions 总被引:2,自引:0,他引:2
A stepwise hydration-equilibrium model for aqueous electrolytes is developed and shown to give a good quantitative account of osmotic coefficients of strong, highly soluble electrolytes up to concentrations of 20–30 m. The main parameters needed are two equilibrium constants describing the stepwise hydration and the number of hydration sites. Choice of ion-size parameter has only a minor effect.This paper was presented at the symposium, The Physical Chemistry of Aqueous Systems, held at the University of Pittsburgh, Pittsburgh, Pennsylvania, June 12–14, 1972, in honor of the 70th birthday of Professor H. S. Frank.Contribution from the Diffusion Research Unit, Research School of Physical Sciences, Australian National University, Canberra, A.C.T., Australia and the University of New England, Armidale, N. S. W., Australia. 相似文献
104.
Using the isothermal displacement calorimeter, enthalpies of dilution are obtained for solutions of urea in water, methanol, ethanol, formamide, N,N-dimethyl-formamide, and dimethyl sulfoxide. The results are discussed in terms of solute-solvent and solute-solute interactions.Now Diane Beaumont. 相似文献
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106.
Vijay K. Stokes 《Zeitschrift für Angewandte Mathematik und Physik (ZAMP)》1989,40(2):201-215
The structure of the principal directions of stress is characterized for the general case of asymmetric stress. It is shown that, for asymmetric stress, the principal directions can comprise either only one direction, or only two directions, or only three directions, or a plane of directions, or a plane of directions together with a direction external to this plane. The asymmetric stress obtained by combining a one-dimensional stress field with a skew-symmetric stress field is analyzed in detail. 相似文献
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