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排序方式: 共有433条查询结果,搜索用时 15 毫秒
31.
Helene?Bonkerud?Dugstad Nickolaj?Jacob?PetersenEmail author Henrik?Jensen Charlotte?Gabel-Jensen Steen?Honoré?Hansen Stig?Pedersen-Bjergaard 《Analytical and bioanalytical chemistry》2014,406(2):421-429
A small and very simple electromembrane extraction probe (EME-probe) was developed and coupled directly to electrospray ionization mass spectrometry (ESI-MS), and this system was used to monitor in real time in vitro metabolism by rat liver microsomes of drug substances from a small reaction (incubation) chamber (37 °C). The drug-related substances were continuously extracted from the 1.0 mL metabolic reaction mixture and into the EME-probe by an electrical potential of 2.5 V. The extraction probe consisted of a 1-mm long and 350-μm ID thin supported liquid membrane (SLM) of 2-nitrophenyl octyl ether. The drugs and formed metabolites where extracted through the SLM and directly into a 3 μL min?1 flow of 60 mM HCOOH inside the probe serving as the acceptor solution. The acceptor solution was directed into the ESI-MS-system, and the MS continuously monitored the drug-related substances extracted by the EME-probe. The extraction efficiency of the EME-probe was dependant on the applied electrical potential and the length of the SLM, and these parameters as well as the volume of the reaction chamber were set to the values mentioned above to avoid serious depletion from the reaction chamber (soft extraction). Soft extraction was mandatory in order not to affect the reaction kinetics by sample composition changes induced by the EME-probe. The EME-probe/MS-system was used to establish kinetic profiles for the in vitro metabolism of promethazine, amitriptyline and imipramine as model substances. 相似文献
32.
A laboratory system for the on-line monitoring of important lactic acid fermentation variables is described. The system contains flow-injection analysers for glucose, lactose, galactose, lactate and protein and a continuous-flow analyser for the biomass concentration. The sugar and lactate analysers are based on enzymatic reactions involving oxidases followed by chemiluminescence detection of the hydrogen peroxide formed. The protein analyser is based on the biuret reaction. The system has been used to monitor many fermentation experiments, and some results are presented as examples. 相似文献
33.
34.
P. Kluczynski S. Lundqvist J. Westberg O. Axner 《Applied physics. B, Lasers and optics》2011,103(2):451-459
A Faraday modulation spectrometer for sensitive and fast detection of nitric oxide at 5.33 μm utilizing a room temperature
continuous wave distributed feedback quantum cascade laser and a Peltier cooled MCT detector is presented. The magnetic field
was modulated at 7.4 kHz whereas the laser wavelength was scanned at 20 Hz across the most favorable rotational-vibrational
transition for FAMOS, Q
3/2(3/2), at 5.33 μm. Using a 15 cm optical path and lineshape fitting, the spectrometer provides a detection limit of 4.5 ppb
for a response time of 1 s. An Allan variance analysis demonstrates that the system has an excellent stability, up to several
hours of operation. 相似文献
35.
Skorodumova NV Simak SI Lundqvist BI Abrikosov IA Johansson B 《Physical review letters》2002,89(16):166601
The microscopic mechanism behind the extraordinary ability of ceria to store, release, and transport oxygen is explained on the basis of first-principles quantum mechanical simulations. The oxygen-vacancy formation energy in ceria is calculated for different local environments. The reversible CeO2-Ce2O3 reduction transition associated with oxygen-vacancy formation and migration is shown to be directly coupled with the quantum process of electron localization. 相似文献
36.
Andersen Sune K. Johannessen Tue Mosleh Majid Wedel Stig Tranto Janne Livbjerg Hans 《Journal of nanoparticle research》2002,4(5):405-416
Flame-generated aerosol particles of Al2O3 were deposited by gas filtration on two types of porous and ceramic tubes of -Al2O3 with mean pore diameters of 450 and 2700nm, respectively. The particles were aggregates with average mobility diameters in the range of 30–100nm and primary particle diameters of 4–8nm. The particles are characterized by differential mobility analysis, transmission electron microscopy, and by their specific surface area. The deposited membranes are characterized by gas permeability measurements, scanning electron microscopy, and by their pore size distribution from nitrogen capillary condensation. The particles form a distinct, homogeneous membrane layer with a porosity of 90% on top of the substrate surface and only penetrate slightly into the substrate structure. The mean pore sizes of the deposited membranes determined by nitrogen condensation agree approximately with those determined by gas permeation and the specific surface area. The mean pore diameter varies in the range of 30–70nm. The gas permeability of the deposited membranes is related to the specific surface area but influenced by the high porosity. The mean pore size and the permeability of the membranes are almost independent of the substrate structure.The development of a membrane with uniform properties is preceded by a short initial period in which the deposited particles, with an equivalent membrane thickness of roughly 2m, have a significantly lower permeability than the ultimately developed uniform membrane layer. This effect is particularly significant for the aerosol particles with the lowest mean size, probably due to particles deposited in the pore mouths of the substrate.The particles and the deposited membranes are X-ray amorphous but retain their specific surface area on heating to even high temperatures. When the membranes are heated to 1473K for 10h, X-ray diffraction shows a mixture of - and -alumina, accompanied by a partial disintegration of the membrane and a considerable loss of surface area. 相似文献
37.
Stig Flodmark 《International journal of quantum chemistry》1967,1(2):147-159
An electronic-computer programme has been written by the author for calculations of energy bands in the tight-binding approximation. This programme has been applied to crystals of α zirconium with and without dissolved oxygen. The resulting energy bands and orbital distributions represent an enormous amount of numerical data. Another programme has now also been written by the author for population analysis of the occupied bands. This programme will reduce the resulting output considerably, and only occupied bands are represented in a number of directions in k space. The occupation numbers are summed up for orbitals with equal quantum numbers l and |m|, and are found for each band as a function of k . 相似文献
38.
Protein adsorption onto silica nanoparticles: conformational changes depend on the particles' curvature and the protein stability 总被引:6,自引:0,他引:6
Lundqvist M Sethson I Jonsson BH 《Langmuir : the ACS journal of surfaces and colloids》2004,20(24):10639-10647
We have analyzed the adsorption of protein to the surfaces of silica nanoparticles with diameters of 6, 9, and 15 nm. The effects upon adsorption on variants of human carbonic anhydrase with differing conformational stabilities have been monitored using methods that give complementary information, i.e., circular dichroism (CD), nuclear magnetic resonance (NMR), analytical ultracentrifugation (AUC), and gel permeation chromatography. Human carbonic anhydrase I (HCAI), which is the most stable of the protein variants, establishes a dynamic equilibrium between bound and unbound protein following mixture with silica particles. Gel permeation and AUC experiments indicate that the residence time of HCAI is on the order of approximately 10 min and slowly increases with time, which allows us to study the effects of the interaction with the solid surface on the protein structure in more detail than would be possible for a process with faster kinetics. The effects on the protein conformation from the interaction have been characterized using CD and NMR measurements. This study shows that differences in particle curvature strongly influence the amount of the protein's secondary structure that is perturbed. Particles with a longer diameter allow formation of larger particle-protein interaction surfaces and cause larger perturbations of the protein's secondary structure upon interaction. In contrast, the effects on the tertiary structure seem to be independent of the particles' curvature. 相似文献
39.
Rydberg H Dion M Jacobson N Schröder E Hyldgaard P Simak SI Langreth DC Lundqvist BI 《Physical review letters》2003,91(12):126402
To understand sparse systems, we must account for both strong local atom bonds and weak nonlocal van der Waals forces between atoms separated by empty space. A fully nonlocal functional form [Phys. Rev. B 62, 6997 (2000)]] of density-functional theory (DFT) is applied here to the layered systems graphite, boron nitride, and molybdenum sulfide to compute bond lengths, binding energies, and compressibilities. These key examples show that the DFT with the generalized-gradient approximation does not apply for calculating properties of sparse matter, while use of the fully nonlocal version appears to be one way to proceed. 相似文献
40.
A. Sjöström V. Tolmachev O. Lebeda J. Koziorowski J. Carlsson H. Lundqvist 《Journal of Radioanalytical and Nuclear Chemistry》2003,256(2):191-197
We have investigated a method for direct astatine labeling of proteins. Binding sites for astatine were created by coupling of a nido-carborane derivative to a protein, the human epidermal growth factor (hEGF), using two different conjugation methods - by glutaraldehyde cross-linking or by introduction of sulfohydryl groups by Traut's reagent with subsequent linking of ANC-1 with m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester. The conjugates were astatinated using the Chloramine-T method in high yield. The best labeling was obtained by the glutaraldehyde conjugate with an average yield of 68±9%. In vitro stability tests indicated that the glutaraldehyde conjugated label was as stable as hEGF labeled with astatobenzoate. 相似文献