Application of high-resolution magic-angle spinning NMR spectroscopy to define the cell uptake of MRI contrast agents

A new method, based on proton high-resolution magic-angle spinning ((1)H HR-MAS) NMR spectroscopy, has been employed to study the cell uptake of magnetic resonance imaging contrast agents (MRI-CAs). The method was tested on human red blood cells (HRBC) and white blood cells (HWBC) by using three gadolinium complexes, widely used in diagnostics, Gd-BOPTA, Gd-DTPA, and Gd-DOTA, and the analogous complexes obtained by replacing Gd(III) with Dy(III), Nd(III), and Tb(III) (i.e., complexes isostructural to the ones of gadolinium but acting as shift agents). The method is based on the evaluation of the magnetic effects, line broadening, or induced lanthanide shift (LIS) caused by these complexes on NMR signals of intra- and extracellular water. Since magnetic effects are directly linked to permeability, this method is direct. In all the tests, these magnetic effects were detected for the extracellular water signal only, providing a direct proof that these complexes are not able to cross the cell membrane. Line broadening effects (i.e., the use of gadolinium complexes) only allow qualitative evaluations. On the contrary, LIS effects can be measured with high precision and they can be related to the concentration of the paramagnetic species in the cellular compartments. This is possible because the HR-MAS technique provides the complete elimination of bulk magnetic susceptibility (BMS) shift and the differentiation of extra- and intracellular water signals. Thus with this method, the rapid quantification of the MRI-CA amount inside and outside the cells is actually feasible.     

Physical anomalous dimensions at small x

I present a theoretical discussion of the uncertainties related to the QCD analysis of the proton structure function F ₂(x,Q ²) at small x. The role played by the ‘unphysical’ gluon density is pointed out. It is shown how the study of more observables can reduce the theoretical uncertainty and, in particular, an alternative method of analysis, based on the introduction of physical anomalous dimensions, is suggested.     

An iterative adaptive finite element method for elliptic eigenvalue problems

We consider the task of resolving accurately the n$n$th eigenpair of a generalized eigenproblem rooted in some elliptic partial differential equation (PDE), using an adaptive finite element method (FEM). Conventional adaptive FEM algorithms call a generalized eigensolver after each mesh refinement step. This is not practical in our situation since the generalized eigensolver needs to calculate n$n$ eigenpairs after each mesh refinement step, it can switch the order of eigenpairs, and for repeated eigenvalues it can return an arbitrary linear combination of eigenfunctions from the corresponding eigenspace. In order to circumvent these problems, we propose a novel adaptive algorithm that only calls a generalized eigensolver once at the beginning of the computation, and then employs an iterative method to pursue a selected eigenvalue–eigenfunction pair on a sequence of locally refined meshes. Both Picard’s and Newton’s variants of the iterative method are presented. The underlying partial differential equation (PDE) is discretized with higher-order finite elements (hp$hp$-FEM) but the algorithm also works for standard low-order FEM. The method is described and accompanied with theoretical analysis and numerical examples. Instructions on how to reproduce the results are provided.     

Reversibility in Infinite Hamiltonian Systems with Conservative Noise

The set of stationary measures of an infinite Hamiltonian system with noise is investigated. The model consists of particles moving in with bounded velocities and subject to a noise that does not violate the classical laws of conservation, see [OVY]. Following [LO] we assume that the noise has also a finite radius of interaction, and prove that translation invariant stationary states of finite specific entropy are reversible with respect to the stochastic component of the evolution. Therefore the results of [LO] imply that such invariant measures are superpositions of Gibbs states. Received: 26 September 1996 / Accepted: 3 January 1997     

Complex Geometry and Dirac Equation

Complex geometry represents a fundamentalingredient in the formulation of the Dirac equation bythe Clifford algebra. The choice of appropriate complexgeometries is strictly related to the geometricinterpretation of the complex imaginary unit . We discuss two possibilities which appearin the multivector algebra approach: the₁₂₃ and ₂₁ complexgeometries. Our formalism provides a set of rules which allows an immediate translation between thecomplex standard Dirac theory and its version withingeometric algebra. The problem concerning a doublegeometric interpretation for the complex imaginary unit is also discussed.     

Cosmological dark energy effects from entanglement

The thorny issue of relating information theory to cosmology is here addressed by assuming a possible connection between quantum entanglement measures and observable universe. In particular, we propose a cosmological toy model, where the equation of state of the cosmological fluid, which drives the today observed cosmic acceleration, can be inferred from quantum entanglement between different cosmological epochs. In such a way the dynamical dark energy results as byproduct of quantum entanglement.     

Effectivity Functions and Stable Governance Structures

Stability properties of several governance structures are studied by means of their effectivity functions. It is shown that (strong) stability of a governance structure is mainly determined by the collegial nature of the underlying assembly's decision rule.     

Soft Constraint Logic Programming and Generalized Shortest Path Problems

In this paper we study the relationship between Constraint Programming (CP) and Shortest Path (SP) problems. In particular, we show that classical, multicriteria, partially ordered, and modality-based SP problems can be naturally modeled and solved within the Soft Constraint Logic Programming (SCLP) framework, where logic programming is coupled with soft constraints. In this way we provide this large class of SP problems with a high-level and declarative linguistic support whose semantics takes care of both finding the cost of the shortest path(s) and also of actually finding the path(s). On the other hand, some efficient algorithms for certain classes of SP problems can be exploited to provide some classes of SCLP programs with an efficient way to compute their semantics.     

The influence of track modelling options on the simulation of rail vehicle dynamics

This paper investigates the effect of different models for track flexibility on the simulation of railway vehicle running dynamics on tangent and curved track. To this end, a multi-body model of the rail vehicle is defined including track flexibility effects on three levels of detail: a perfectly rigid pair of rails, a sectional track model and a three-dimensional finite element track model. The influence of the track model on the calculation of the nonlinear critical speed is pointed out and it is shown that neglecting the effect of track flexibility results in an overestimation of the critical speed by more than 10%. Vehicle response to stochastic excitation from track irregularity is also investigated, analysing the effect of track flexibility models on the vertical and lateral wheel–rail contact forces. Finally, the effect of the track model on the calculation of dynamic forces produced by wheel out-of-roundness is analysed, showing that peak dynamic loads are very sensitive to the track model used in the simulation.