Highly sensitive LC-MS/MS-ESI method for simultaneous quantitation of albendazole and ricobendazole in rat plasma and its application to a rat pharmacokinetic study

A highly sensitive and specific LC-MS/MS-ESI method was developed for simultaneous quantification of albenadazole (ABZ) and ricobendazole (RBZ) in rat plasma (50 μL) using phenacetin as an internal standard (IS). Simple protein precipitation was used to extract ABZ and RBZ from rat plasma. The chromatographic resolution of ABZ, RBZ and IS was achieved with a mobile phase consisting of 5 m m ammonium acetate (pH 6) and acetonitrile (20:80, v/v) at a flow rate of 1 mL/min on a Chromolith RP-18e column. The total chromatographic run time was 3.5 min and the elution of ABZ, RBZ and IS occurred at 1.66, 1.50 and 1.59 min, respectively. A linear response function was established for the ranges of concentrations 2.01-2007 and 6.02-6020 ng/mL for ABZ and RBZ, respectively. The intra- and inter-day precision values for ABZ and RBZ met the acceptance as per FDA guidelines. ABZ and RBZ were stable in battery of stability studies, viz. bench-top, auto-sampler and freeze-thaw cycles. The developed assay was applied to a pharmacokinetic study in rats.     

Minimum Hellinger distance estimation for supercritical Galton–Watson processes

This paper studies the asymptotic behavior of the minimum Hellinger distance estimator of the underlying parameter in a supercritical branching process whose offspring distribution is known to belong to a parametric family. This estimator is shown to be asymptotically normal, efficient at the true model and robust against gross errors. These extend the results of Beran (Ann. Statist. 5, 445–463 (1977)) from an i.i.d., continuous setup to a dependent, discrete setup.     

A potassium–18-crown-6 salt of a cyclic myo-inositol phosphate

The six-membered phosphorinane ring in (1,4,7,10,13,16-hexaoxa­cyclo­octa­decane)­potassium 2-O-benzoyl-1,3,5-O-methyl­idyne-myo-in­osi­tol 4,6-cyclo­phosphate trihydrate, [K(C₁₂H₂₄O₆)](C₁₄H₁₂O₉P)·3H₂O, has a boat rather than a chair conformation. The K⁺ ion is eight-coordinate and is connected to one of the phosphate O atoms, one of the O atoms of the myo-inositol residue and the six O atoms of the crown ether.     

Amino Acid-Derived Sensors for Specific Zn2+ Detection Using Hyperpolarized 13C Magnetic Resonance Spectroscopy

Alterations in Zn²⁺ concentration are seen in normal tissues and in disease states, and for this reason imaging of Zn²⁺ is an area of active investigation. Herein, enriched [1-¹³C]cysteine and [1-¹³C₂]iminodiacetic acid were developed as Zn²⁺-specific imaging probes using hyperpolarized ¹³C magnetic resonance spectroscopy. [1-¹³C]cysteine was used to accurately quantify Zn²⁺ in complex biological mixtures. These sensors can be employed to detect Zn²⁺ via imaging mechanisms including changes in ¹³C chemical shift, resonance linewidth, or T₁.     

Setting sensitivity in Talbot interferometry with modified gratings

A dual field grating has been introduced for use in Talbot interferometry. We report here results of an experimental investigation on setting sensitivity when using these gratings for testing.     

Novel routes for the generation of structurally diverse labdane diterpenes from andrographolide

Andrographolide 1, the major constituent of the Indian medicinal plant Andrographis paniculata (Acanthaceae) was converted into the key intermediate 4 by selective oxidative degradation of the C-12,13 olefin bond. The aldehyde functional group present in 4 has been utilized for synthesizing a number of structurally diverse labdane diterpenes. Synthesis and in vitro cytotoxic activity results of the compounds prepared are discussed.     

Synthesis of side chain truncated 3"-aldehyde, 3"-carboxylic acid, and 1"-aldehyde from nodulisporic acid A

An efficient synthesis of the truncated 3"-aldehyde (3) from nodulisporic acid A (1) under mild conditions is described. Further oxidation of 3 to 3"-carboxylic acid (4) and its subsequent oxidative degradation produced 1"-aldehyde (5). These new derivatives are versatile intermediates for the preparation of new, side chain modified derivatives of nodulisporic acid A. [reaction: see text]     

Light‐Triggered,Spatially Localized Chemistry by Photoinduced Electron Transfer

It is of immense interest to exert spatial and temporal control of chemical reactions. It is now demonstrated that irradiation can trigger reactions specifically at the surface of a simple colloidal construct, obtained by adsorbing polyethyleneimine on fluorescent colloidal particles. Exciting the fluorescent dye in the colloid affords photoinduced electron transfer to spatially proximal amine groups on the adsorbed polymer to form free radical ions. It is demonstrated that these can be harnessed to polymerize acrylic acid monomer at the particle surface, or to break up colloidal assemblies by cleaving a cross‐linked polymer mesh. Formation of free radical ions is not a function of the size of the colloid, neither is it restricted to a specific fluorophore. Fluorophores with redox potentials that allow photoinduced electron transfer with amine groups show formation of free radical ions.     

Quantification of phase shift in the simulation of shallow water waves

Numerical simulation of nonlinear waves to reproduce the laboratory measurements has been a topic of great interest in the recent past. The results reported in the literature are mainly focused on qualitative comparison or on the relative errors between the numerical simulation and measurements in laboratory and hence lack in revealing the existence of phase shift in nonlinear wave simulation. In this paper, the simulation of nonlinear waves in mixed Eulerian and Lagrangian framework using finite element method (FEM) is investigated by applying two different velocity calculation methods viz, cubic spline and least squares (LS). The simulated wave surface elevation has been compared with the experimental measurements. The coherence analysis has been carried out using the wavelet transformation, which gives a better understanding between the numerical and the experimental results with respect to the time–frequency space, compared with the conventional Fourier transformation. It is observed that the application of cubic spline approach leads to a higher phase difference for steeper waves. The present study has shown that the phase difference exists at the higher modes rather than at the primary period. For waves with steepness (wave height/wave length) higher than 0.04, LS approach is found to be effective in capturing the higher‐order frequency components in the event of nonlinearity. In addition, the comparison of numerical simulations with that from PIV measurements for the tests with solitary waves is also reported. Copyright © 2009 John Wiley & Sons, Ltd.