Canine Parvovirus Type 2a (CPV-2a)-Induced Apoptosis in MDCK Involves Both Extrinsic and Intrinsic Pathways

The canine parvovirus type 2 (CPV-2) causes an acute disease in dogs. It has been found to induce cell cycle arrest and DNA damage leading to cellular lysis. In this paper, we evaluated the apoptotic potential of the “new CPV-2a” in MDCK cells and elucidated the mechanism of the induction of apoptosis. The exposure of MDCK cells to the virus was found to trigger apoptotic response. Apoptosis was confirmed by phosphatidylserine translocation, DNA fragmentation assays, and cell cycle analysis. Activation of caspases-3, -8, -9, and -12 and decrease in mitochondrial potential in CPV-2a-infected MDCK cells suggested that the CPV-2a-induced apoptosis is caspase dependent involving extrinsic, intrinsic, and endoplasmic reticulum pathways. Increase in p53 and Bax/Bcl2 ratio was also observed in CPV-2a-infected cells.     

A PASE-based approach towards 12-(1H-1,2,3-triazol-1-yl)-indolo[2,1-a]isoquinolines via the reaction of 3-(isoquinolin-1-yl)-1,2,4-triazines with benzyne

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Three-step model for high-harmonic generation in many-electron systems

The three-step model (TSM) of high-harmonic generation (HHG) is generalized to atomic and molecular many-electron systems. Using many-body perturbation theory, corrections to the standard TSM due to exchange and electron-electron correlations are derived. It is shown that canonical Hartree-Fock orbitals represent the most appropriate set of one-electron states for calculating the HHG spectrum. To zeroth order in many-body perturbation theory, a HHG experiment allows direct access, in general, to a combination of occupied Hartree-Fock orbitals rather than to the highest occupied molecular orbital by itself.     

Thin film flow over a non-linear stretching sheet in presence of uniform transverse magnetic field

A thin viscous liquid film flow is developed over a stretching sheet under different non-linear stretching velocities in presence of uniform transverse magnetic field. Evolution equation for the film thickness is derived using long-wave approximation of thin liquid film and is solved numerically by using the Newton–Kantorovich method. It is observed that all types of stretching produces film thinning, but non-monotonic stretching produces faster thinning at small distance from the origin. Effect of the transverse magnetic field is to slow down the film thinning process. Observed flow behavior is explained physically.     

TAT conjugated, FITC doped silica nanoparticles for bioimaging applications

Water-in-oil (w/o) microemulsion synthesis of 70 nm size monodisperse TAT (a cell penetrating peptide, CPP) conjugated, FITC (fluorescein isothiocyanate) doped silica nanoparticles (TAT-FSNPs) is reported; human lung adenocarcinoma (A549) cells (in vitro) and rat brain tissue (in vivo) were successfully labeled using TAT-FSNPs.     

Robust reliable L2 – L∞ control for continuous‐time systems with nonlinear actuator failures

This article examines the reliable L₂ – L_∞ control design problem for a class of continuous‐time linear systems subject to external disturbances and mixed actuator failures via input delay approach. Also, due to the occurrence of nonlinear circumstances in the control input, a more generalized and practical actuator fault model containing both linear and nonlinear terms is constructed to the addressed control system. Our attention is focused on the design of the robust state feedback reliable sampled‐data controller that guarantees the robust asymptotic stability of the resulting closed‐loop system with an L₂ – L_∞ prescribed performance level γ > 0, for all the possible actuator failure cases. For this purpose, by constructing an appropriate Lyapunov–Krasovskii functional (LKF) and utilizing few integral inequality techniques, some novel sufficient stabilization conditions in terms of linear matrix inequalities (LMIs) are established for the considered system. Moreover, the established stabilizability conditions pave the way for designing the robust reliable sampled‐data controller as the solution to a set of LMIs. Finally, as an example, a wheeled mobile robot trailer model is considered to illustrate the effectiveness of the proposed control design scheme. © 2016 Wiley Periodicals, Inc. Complexity 21: 309–319, 2016     

On the accuracy of density-functional theory exchange-correlation functionals for H bonds in small water clusters: benchmarks approaching the complete basis set limit

The ability of several density-functional theory (DFT) exchange-correlation functionals to describe hydrogen bonds in small water clusters (dimer to pentamer) in their global minimum energy structures is evaluated with reference to second order Moller-Plesset perturbation theory (MP2). Errors from basis set incompleteness have been minimized in both the MP2 reference data and the DFT calculations, thus enabling a consistent systematic evaluation of the true performance of the tested functionals. Among all the functionals considered, the hybrid X3LYP and PBE0 functionals offer the best performance and among the nonhybrid generalized gradient approximation functionals, mPWLYP and PBE1W perform best. The popular BLYP and B3LYP functionals consistently underbind and PBE and PW91 display rather variable performance with cluster size.