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951.
952.
Rapid quantitative measurements of proteomes by Fourier transform ion cyclotron resonance mass spectrometry. 总被引:1,自引:0,他引:1
R D Smith L Pasa-Toli? M S Lipton P K Jensen G A Anderson Y Shen T P Conrads H R Udseth R Harkewicz M E Belov C Masselon T D Veenstra 《Electrophoresis》2001,22(9):1652-1668
The patterns of gene expression, post-translational modifications, protein/biomolecular interactions, and how these may be affected by changes in the environment, cannot be accurately predicted from DNA sequences. Approaches for proteome characterization are generally based upon mass spectrometric analysis of in-gel digested two dimensional polyacrylamide gel electrophoresis (2-D PAGE) separated proteins, allowing relatively rapid protein identification compared to conventional approaches. This technique, however, is constrained by the speed of the 2-D PAGE separations, the sensitivity limits intrinsic to staining necessary for protein visualization, the speed and sensitivity of subsequent mass spectrometric analyses for identification, and the limited ability for accurate quantitative measurements based on differences in spot intensity. We are presently developing alternative approaches for proteomics based upon the combination of fast capillary electrophoresis, or other suitable chromatographic separations, and the high mass accuracy and sensitivity obtainable with unique Fourier transform ion cyclotron resonance (FTICR) mass spectrometers available at our laboratory. Several approaches are presently being pursued; one based upon the analysis of intact proteins and the second upon approaches for global protein digestion and accurate peptide mass analysis. Quantitation of protein/peptide levels are based on using two or more stable-isotope labeled versions of proteomes which are combined to obtain precise quantitation of relative protein abundances. We describe the status of our efforts towards the development of a high-throughput proteomics capability and present initial results for application to several microorganisms and discuss our efforts for extending the developed capability to mammalian proteomes. 相似文献
953.
The problem of a linear viscoelastic body, containing a penny-shaped crack subjected to the shear parallel to the edge of the crack is considered in this paper. Closed form expressions for the displacements over the surface of the crack, the shear components in the plane of the crack and the stress intensity factors are determined. The various expressions are then specialized for two particular linear viscoelastic materials and the effect of viscoelasticity, wherever possible, is pointed out. 相似文献
954.
955.
956.
Jerald K. Rasmussen Steven M. Heilmann Larry R. Krepski Howell K. Smith Alan R. Katritzky Kumars Sakizadeh 《Journal of polymer science. Part A, Polymer chemistry》1986,24(11):2739-2747
The reaction of bisazlactones (2-oxazolin-5-ones) with primary diamines containing additional secondary or tertiary amine functionality (e.g., diethylenetriamine, triethylenetetramine, or N-methyliminobispropylamine) readily produces polyamides which serve as precursors to a new class of heterocyclic polymers. Thermal cyclodehydration takes place under relatively mild conditions (180–200°C) to produce water-soluble polymers containing the 2-imidazolin-5-one heterocycle. Model reactions have been studied to verify this mode of cyclization and confirm the proposed polymer structure. 相似文献
957.
E. D. Minor T. A. Armstrong R. Bishop V. Harris R. A. Lewis G. A. Smith 《Zeitschrift für Physik A Hadrons and Nuclei》1990,336(4):461-468
The momentum spectra of charged pions following antiproton annihilation at rest in carbon and uranium have been measured. This information complements our previous measurement of the neutral pion spectra. The total charged pion multiplicity is 2.84±0.10 and 2.47±0.09 for carbon and uranium, respectively, in good agreement with recent INC model predictions of 2.96 and 2.48 for the same quantities. However, structures predicted by the model near 200 MeV/c and 300 MeV/c related to delta-resonance production are not seen in the data. The total energy transfers to the nucleus are calculated to be 119±59 MeV(carbon) and 455±50 MeV(uranium). The possibility of exciting multifragmentation with a¯p beam impinging on heavy nuclei is discussed. 相似文献
958.
An earlier paper has put forward a methodology for measuring the ‘need to spend’ on education by local authorities. This paper examines the economic principles underlying the measurement of the need to spend, and finds some problems inherent in the earlier work. The measurement of spending need would nevertheless seem an essential step in the pursuit of territorial equity, particularly in the light of policy developments in the United Kingdom concerning local management of schools. The paper therefore identifies the areas where further clarification of key issues is required. 相似文献
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960.
This article summarizes the basic concepts of multiplication and provides some evidence that the traditional third‐grade curriculum and instruction emphasizing memorization of multiplication facts produces much less understanding of the basic concepts of multiplication than a standards‐based curriculum and instruction emphasizing construction of number sense and meaning for operations. This study also describes a collection of assessment tasks that provided meaningful evidence of children's understandings of basic multiplication concepts, including understandings of the relationships between multiplication and addition. 相似文献