The use of selected ion flow tube mass spectrometry to detect and quantify polyamines in headspace gas and oral air

Polyamines are a class of aliphatic compounds which include putrescine, cadaverine, spermine and spermidine. They are involved in a variety of cellular processes and have been implicated in a number of different pathophysiological mechanisms. Polyamines are volatile compounds having a distinctive odour normally perceived as being unpleasant. The measurement of their abundance has, however, been restricted to compounds present in the aqueous phase. Using selected ion flow tube mass spectrometry (SIFT‐MS) we have shown that the polyamines react with the ions H₃O⁺, NO⁺ and O to form distinctive product ions allowing their levels to be quantified in the vapour phase. The low volatility of spermine did not allow extensive analysis of this compound by SIFT‐MS while the adherent properties of cadaverine and putrescine required the use of PTFE transfer lines and couplers. Our data suggested the presence of cadaverine and putrescine in both oral air and the headspace of putrefying bovine muscle, while product ions corresponding to putrescine and spermidine were found in the headspace of human semen. SIFT‐MS therefore appears to be a practical means of measuring vapour‐phase polyamine levels, having applications in biology, medicine and dentistry, and food science. Copyright © 2009 John Wiley & Sons, Ltd.     

Inhibition of Fungi and Gram-Negative Bacteria by Bacteriocin BacTN635 Produced by Lactobacillus plantarum sp. TN635

The aim of this study was to evaluate 54 lactic acid bacteria (LAB) strains isolated from meat, fermented vegetables and dairy products for their capacity to produce antimicrobial activities against several bacteria and fungi. The strain designed TN635 has been selected for advanced studies. The supernatant culture of this strain inhibits the growth of all tested pathogenic including the four Gram-negative bacteria (Salmonella enterica ATCC43972, Pseudomonas aeruginosa ATCC 49189, Hafnia sp. and Serratia sp.) and the pathogenic fungus Candida tropicalis R2 CIP203. Based on the nucleotide sequence of the 16S rRNA gene of the strain TN635 (1,540 pb accession no FN252881) and the phylogenetic analysis, we propose the assignment of our new isolate bacterium as Lactobacillus plantarum sp. TN635 strain. Its antimicrobial compound was determined as a proteinaceous substance, stable to heat and to treatment with surfactants and organic solvents. Highest antimicrobial activity was found between pH 3 and 11 with an optimum at pH?=?7. The BacTN635 was purified to homogeneity by a four-step protocol involving ammonium sulfate precipitation, centrifugal microconcentrators with a 10-kDa membrane cutoff, gel filtration Sephadex G-25, and C18 reverse-phase HPLC. SDS-PAGE analysis of the purified BacTN635, revealed a single band with an estimated molecular mass of approximately 4 kDa. The maximum bacteriocin production (5,000 AU/ml) was recorded after a 16-h incubation in Man, Rogosa, and Sharpe (MRS) medium at 30?°C. The mode of action of the partial purified BacTN635 was identified as bactericidal against Listeria ivanovii BUG 496 and as fungistatic against C. tropicalis R2 CIP203.     

Incorporation of phonetic constraints in acoustic-to-articulatory inversion

This study investigates the use of constraints upon articulatory parameters in the context of acoustic-to-articulatory inversion. These speaker independent constraints, referred to as phonetic constraints, were derived from standard phonetic knowledge for French vowels and express authorized domains for one or several articulatory parameters. They were experimented on in an existing inversion framework that utilizes Maeda's articulatory model and a hypercubic articulatory-acoustic table. Phonetic constraints give rise to a phonetic score rendering the phonetic consistency of vocal tract shapes recovered by inversion. Inversion has been applied to vowels articulated by a speaker whose corresponding x-ray images are also available. Constraints were evaluated by measuring the distance between vocal tract shapes recovered through inversion to real vocal tract shapes obtained from x-ray images, by investigating the spreading of inverse solutions in terms of place of articulation and constriction degree, and finally by studying the articulatory variability. Results show that these constraints capture interdependencies and synergies between speech articulators and favor vocal tract shapes close to those realized by the human speaker. In addition, this study also provides how acoustic-to-articulatory inversion can be used to explore acoustical and compensatory articulatory properties of an articulatory model.     

Estimating the control parameters of an articulatory model from electromagnetic articulograph data

Finding the control parameters of an articulatory model that result in given acoustics is an important problem in speech research. However, one should also be able to derive the same parameters from measured articulatory data. In this paper, a method to estimate the control parameters of the the model by Maeda from electromagnetic articulography (EMA) data, which allows the derivation of full sagittal vocal tract slices from sparse flesh-point information, is presented. First, the articulatory grid system involved in the model's definition is adapted to the speaker involved in the experiment, and EMA data are registered to it automatically. Then, articulatory variables that correspond to measurements defined by Maeda on the grid are extracted. An initial solution for the articulatory control parameters is found by a least-squares method, under constraints ensuring vocal tract shape naturalness. Dynamic smoothness of the parameter trajectories is then imposed by a variational regularization method. Generated vocal tract slices for vowels are compared with slices appearing in magnetic resonance images of the same speaker or found in the literature. Formants synthesized on the basis of these generated slices are adequately close to those tracked in real speech recorded concurrently with EMA.