Simple photografting method to chemically modify and micropattern the surface of SU-8 photoresist

SU-8 has gained widespread acceptance as a negative photoresist. It is also finding increasing use as a structural material in microanalytical devices. Consequently, methods to tailor the surface properties of SU-8 as well as to micropattern coatings on the surface of SU-8 are needed. The SU-8 photoresist consists of EPON SU-8 resin mixed with the photoacid generator triarylsulfonium hexafluoroantimonate. This photoacid generator can also serve as a photoinitiator generating free radicals when illuminated with UV light. Under the appropriate conditions, sufficient triarylsulfonium hexafluoroantimonate remains within cured SU-8 to act as a source of free radicals and initiate UV-mediated grafting of polymers onto the surface of the SU-8. UV-mediated grafting was used to coat SU-8 surfaces with poly(acrylic acid) and other water-soluble monomers. The SU-8 surface was chemically micropatterned by placing a mask between the UV light and SU-8. The X-Y spatial resolution of micropatterned poly(acrylic acid) on the SU-8 surface was 2 mum. Three applications of these chemically modified SU-8 surfaces were demonstrated. In the first, poly(ethylene glycol) was used to protect the SU-8 from interactions with proteins, yielding a surface resistant to biofouling. In the second demonstration, the SU-8 surface was micropatterned with a cell-resistant layer to guide cellular attachment and growth. In the final application, SU-8 micropallets were encoded with polymer lines. The bar codes were read by either absorbance or fluorescence measurements. Thus, UV-mediated graft polymerization is an efficient and effective method to micropattern coatings onto the surface of SU-8.     

Trapping cells on a stretchable microwell array for single-cell analysis

There is a need for a technology that can be incorporated into routine laboratory procedures to obtain a continuous, quantitative, fluorescence-based measurement of the dynamic behaviors of numerous individual living cells in parallel, while allowing other manipulations, such as staining, rinsing, and even retrieval of targeted cells. Here, we report a simple, low-cost microarray platform that can trap cells for dynamic single-cell analysis of mammalian cells. The elasticity of polydimethylsiloxane (PDMS) was utilized to trap tens of thousands of cells on an array. The PDMS microwell array was stretched by a tube through which cells were loaded on the array. Cells were trapped on the array by removal of the tube and relaxation of the PDMS. Once that was accomplished, the cells remained trapped on the array without continuous application of an external force and permitted subsequent manipulations, such as staining, rinsing, imaging, and even isolation of targeted cells. We demonstrate the utility of this platform by multicolor analysis of trapped cells and monitoring in individual cells real-time calcium flux after exposure to the calcium ionophore ionomycin. Additionally, a proof of concept for target cell isolation was demonstrated by using a microneedle to locally deform the PDMS membrane in order to retrieve a particular cell from the array.     

Alternating projections in CAT(0) spaces

By using recently developed theory which extends the idea of weak convergence into CAT(0) space we prove the convergence of the alternating projection method for convex closed subsets of a CAT(0) space. Given the right notion of weak convergence it turns out that the generalization of the well-known results in Hilbert spaces is straightforward and allows the use of the method in a nonlinear setting. As an application, we use the alternating projection method to minimize convex functionals on a CAT(0) space.     

Experimental investigation of two-phase discharge from a stratified region through a small branch mounted on an inclined wall

Experimental data are presented for the mass flow rate and quality of two-phase discharge through a small branch of diameter d (=6.35 mm) attached normally to an inclined flat plane. The flat plane was situated in a large tank containing a stratified mixture of air and water under pressure (316 kPa) and at room temperature. The plane was inclined through various angles (θ) in increments of 30°, from the outlet-branch orientation being vertically upward through the horizontal to vertically downward. The bulk of the data correspond to seven inclination angles and two test-section-to-separator pressure differences (ΔP) of 11.0 and 115.5 kPa, and for each combination of θ and ΔP, the mass flow rate and quality were measured at different values of the interface level (h) between the onsets of gas and liquid entrainment. Four additional data sets were generated for other values of ΔP in order to confirm certain trends. Influences of these independent variables on the mass flow rate and quality are discussed and normalized plots are presented showing that the data can be collapsed for a wide range of conditions. Comparisons are made between the present data and previous correlations/models and new empirical correlations are formulated and shown to be capable of predicting the present data with good accuracy.     

Property (M) and the weak fixed point property

It is shown that in Banach spaces with the property (M) of Kalton, nonexpansive self mappings of nonempty weakly compact convex sets necessarily have fixed points. The stability of this conclusion under renormings is examined and conditions for such spaces to have weak normal structure are considered.

     

Heliuim-3 mass spectrometry for low-level tritium analysis of environmental samples

Helium-3 (³He) mass spectrometry for the analysis of low-level tritium (³H) concentrations (0.5 to 5 Bq·l^–1) in environmental sample matrices was compared with conventional low-level -decay counting methods. The mass-spectrometry method compared favorably, equalling or surpassing conventional decay-counting methods with respect to most criteria. Additional research and method refinements may make³He mass spectrometry the method of choice for routine, low-level to very-low-level (L<0.5 Bq·l^–1)³H measurements in a wide variety of environmental samples in the future.     

Seperation of neptunium from irradiated uranium targets

A new chemical method based in two separation steps was developed to isolate²³⁵Np from uranium targets irradiated with charged particles. Neptunium and plutonium are separated from uranium and most of the fission products by ion exchange. Then, neptunium is isolated from plutonium and remaining contaminants by extraction chromatography with tributyl phosphate in hydrochloric acid solution. High decontamination was achieved.