Investigation of DNA condensing properties of amphiphilic triblock cationic polymers by atomic force microscopy

Introduction of nucleic acids into cells is an important biotechnology research field which also holds great promise for therapeutic applications. One of the key steps in the gene delivery process is compaction of DNA into nanometric particles. The study of DNA condensing properties of three linear cationic triblock copolymers poly(ethylenimine-b-propylene glycol-b-ethylenimine), namely, LPEI(50)-PPG(36)-LPEI(50), LPEI(19)-PPG(36)-LPEI(19), and LPEI(14)-PPG(68)-LPEI(14), indicates that proper DNA condensation is driven by both the charge and the size of the respective cationic hydrophilic linear polyethylenimine (LPEI) and neutral hydrophobic poly(propylene glycol) (PPG) parts. Atomic force microscopy was used to investigate the interactions of the triblock copolymers with plasmid DNA at the single molecule level and to enlighten the mechanism involved in DNA condensation.     

Comparing the effects of reverberation and of noise on speech recognition in simulated electric-acoustic listening

Cochlear implant users report difficulty understanding speech in both noisy and reverberant environments. Electric-acoustic stimulation (EAS) is known to improve speech intelligibility in noise. However, little is known about the potential benefits of EAS in reverberation, or about how such benefits relate to those observed in noise. The present study used EAS simulations to examine these questions. Sentences were convolved with impulse responses from a model of a room whose estimated reverberation times were varied from 0 to 1 sec. These reverberated stimuli were then vocoded to simulate electric stimulation, or presented as a combination of vocoder plus low-pass filtered speech to simulate EAS. Monaural sentence recognition scores were measured in two conditions: reverberated speech and speech in a reverberated noise. The long-term spectrum and amplitude modulations of the noise were equated to the reverberant energy, allowing a comparison of the effects of the interferer (speech vs noise). Results indicate that, at least in simulation, (1) EAS provides significant benefit in reverberation; (2) the benefits of EAS in reverberation may be underestimated by those in a comparable noise; and (3) the EAS benefit in reverberation likely arises from partially preserved cues in this background accessible via the low-frequency acoustic component.     

Theoretical studies of molecular complexes: a probe into basis set and correlation effects

We investigate the effect of basis set size, correlation effects and interplanar separation on the theoretical electronic structure of stacking complexes of para- and meta-hydroxyaruline with formamidinium cation, constructed as analogs for the complexes of 5- and 6-hydroxytryptamine with imidazolium cation.     

Valence band and Zn 3d energy levels in Me2Zn from photoelectron spectra and pseudopotential ab initio calculations: electric field gradients in gas phase Zn compounds

The He I and X-ray photoelectron spectra of the valence levels and Zn 3d levels in Me₂Zn have been recorded. The orbital ionization potentials are compared with those obtained from our ab initio pseudopotential calculation on Me₂Zn. There is excellent agreement between predicted and observed values for the outer valence orbitals. The Zn 3d level in Me₂Zn in split into five peaks due to the combined effect of spin-orbit splitting and crystal field splitting. The major part of the splitting is due to the asymmetric C⁰₂ crystal field term which transforms like the electric field gradient. The derived C⁰₂ terms for Me₂Zn and ZnCl₂ are ?0.0169 ± 0.0007 eV and ?0.011 eV respectively. The observed and calculated splitting confirms an electrostatic (rather than a bonding) origin. The C⁰₂ value for Me₂Zn is consistent with that observed recently for Me₂Cd.     

An Evolutionary Approach for the Design and Scheduling of Electroplating Facilities

This paper tackles the Cyclic Hoists Scheduling Problem. This problem is often encountered in electroplating facilities when mass production is required. Then a repetitive sequence of moves is searched for the hoists. We more precisely deal with a global optimization problem that simultaneously considers the design and the scheduling of such production lines. It consists in studying systems integrating several transportation resources, called hoists, by minimizing the cycle time, while minimizing the number of hoists used. To achieve these goals, we use an evolutionary approach. The encoding of one solution is based on the representation of the empty moves of the hoists. To evaluate each individual, we propose a linear programming model. This one both verifies the satisfaction of constraints and provides the best cycle time for the considered number of hoists. This contribution describes a promising approach to solving a simple version of this problem, namely cyclic hoist scheduling, based on Evolutionary Algorithms (EAs), which is an optimization method inspired by biological evolution models. The issues of solution encoding and specialised genetic operators with a repair procedure of the infeasible solutions are discussed. Some results are presented with benchmark examples.      

Effects of signal delay on auditory filter shapes derived from psychophysical tuning curves and notched-noise data obtained in simultaneous masking

Psychophysical tuning curves (PTCs) measured in simultaneous masking usually sharpen as a short duration signal is moved from the onset to the temporal center of a longer duration masker. Filter shapes derived from notched-noise maskers have not consistently shown this effect. One possible explanation for this difference is that the signal level is fixed in the PTC paradigm, whereas the masker level is usually fixed in the notched-noise paradigm. In the present study, the signal level was fixed at 10 dB SL in both paradigms. The signal was 20 ms in duration, and presented at the onset or temporal center of the 400-ms masker. The masker was a pure tone presented in quiet (PTC) or in the presence of a pure-tone "restrictor" intended to limit off-frequency listening (PTCr), or it was a noise with a spectral notch placed symmetrically or asymmetrically about the 2-kHz signal frequency. Filter shapes were derived from the PTC, PTCr, and notched-noise data using the roex (p, w, t) model. The effects of signal delay and masking paradigm on filter bandwidth were analyzed with a two-factor repeated-measures ANOVA. There was a significant effect of signal delay (the filters sharpened with time) and masking paradigm (the filters derived from the notched-noise data were significantly wider than those derived from either of the PTC measurements, which did not differ from one another). Although the interaction between delay and paradigm was not significant, the filter derived from the notched-noise data sharpened more with time than did the other filters, and thus the bandwidth of the filters from the three paradigms were more similar at the longer delay than at the shorter delay. It is likely that the tuning-curve and notched-noise paradigms measure the same underlying filtering, but that various other factors contribute differentially to the derived filter shapes.     

Temporal effects in simultaneous masking with on- and off-frequency noise maskers: effects of signal frequency and masker level

Temporal effects in simultaneous masking were measured as a function of masker level for an on-frequency broadband masker and an off-frequency narrow-band masker for signal frequencies of 750, 1730, and 4000 Hz. The on-frequency masker was 10 equivalent rectangular bandwidths (ERBs) wide and centered at the signal frequency; the off-frequency masker was 500 Hz wide and its lower frequency edge was 1.038 ERBs higher in frequency than the signal. The primary goal of the study was to determine whether previously observed differences regarding the effects of signal frequency and masker level on the temporal effect for these two different types of masker might be due to considerably different signal levels at threshold. Despite similar masked thresholds, the effects of signal frequency and masker level in the present study were different for the two masker types. The temporal effect was significant for the two highest frequencies and absent for the lowest frequency in the presence of the broadband masker, but was more or less independent of frequency for the narrow-band masker. The temporal effect increased but then decreased as a function of level for the broadband masker (at the two higher signal frequencies, where there was a temporal effect), but increased and reached an asymptote for the narrow-band masker. Despite the different effects of signal frequency and masker level, the temporal effects for both types of masker can be understood in terms of a basilar-membrane input-output function that becomes more linear during the course of masker stimulation.