A Study on Spectro-Analytical Aspects,DNA – Interaction,Photo-Cleavage,Radical Scavenging,Cytotoxic Activities,Antibacterial and Docking Properties of 3 – (1 – (6 – methoxybenzo [d] thiazol – 2 – ylimino) ethyl) – 6 – methyl – 3H – pyran - 2, 4 – dione and its Metal Complexes

The focus of the present work is on the design, synthesis, characterization, DNA-interaction, photo-cleavage, radical scavenging, in-vitro cytotoxicity, antimicrobial, docking and kinetic studies of Cu (II), Cd (II), Ce (IV) and Zr (IV) metal complexes of an imine derivative, 3 – (1 – (6 – methoxybenzo [d] thiazol – 2 – ylimino) ethyl) – 6 – methyl – 3H – pyran – 2, 4 – dione. The investigation of metal ligand interactions for the determination of composition of metal complexes, corresponding kinetic studies and antioxidant activity in solution was carried out by spectrophotometric methods. The synthesized metal complexes were characterized by EDX analysis, Mass, IR, ¹H-NMR, ¹³C-NMR and UV–Visible spectra. DNA binding studies of metal complexes with Calf thymus (CT) DNA were carried out at room temperature by employing UV-Vis electron absorption, fluorescence emission and viscosity measurement techniques. The results revealed that these complexes interact with DNA through intercalation. The results of in vitro antibacterial studies showed the enhanced activity of chelating agent in metal chelated form and thus inferring scope for further development of new therapeutic drugs. Cell viability experiments indicated that all complexes showed significant dose dependent cytotoxicity in selected cell lines. The molecular modeling and docking studies were carried out with energy minimized structures of metal complexes to identify the receptor to metal interactions.     

“On-water” synthesis of 3-substituted indoles via Knoevenagel/Michael addition sequence catalyzed by Cu doped ZnS NPs

Cu doped ZnS NPs represent a green catalyst for an ‘on-water’ one-pot rapid synthesis of 3-substituted indole derivatives via Knoevenagel/Michael addition reaction of indane-1,3-dione, aromatic aldehydes, and indole. The catalytic activity of Cu doped ZnS NPs was about sevenfold higher as compared to the ZnS NPs. The Cu doped ZnS NPs catalyst could be recovered and reused for five reaction cycles, giving a total TOF = 201 h⁻¹.     

Convenient,One-Step Syntheses of 2-Fluoroveratrole And 2-Fluoroveratraldehyde

Metalated veratraldehyde and veratrole were quenched with perchloryl fluoride to provide one-step syntheses of 2-fluoroveratraldehyde and 2-fluoroveratrole, respectively. Previous syntheses of these precursors to 2-fluorodopamine and 5-fluoronorepinephrine are multi-step processess which involve introduction of fluorine at an early stage. Perchloryl fluoride is a potentially hazardous reagent, and should be used only on an appropriate (millimolar) scale with precautions.     

Altering protein surface charge with chemical modification modulates protein–gold nanoparticle aggregation

Gold nanoparticles (AuNP) can interact with a wide range of molecules including proteins. Whereas significant attention has focused on modifying the nanoparticle surface to regulate protein–AuNP assembly or influence the formation of the protein “corona,” modification of the protein surface as a mechanism to modulate protein–AuNP interaction has been less explored. Here, we examine this possibility utilizing three small globular proteins—lysozyme with high isoelectric point (pI) and established interactions with AuNP; α-lactalbumin with similar tertiary fold to lysozyme but low pI; and myoglobin with a different globular fold and an intermediate pI. We first chemically modified these proteins to alter their charged surface functionalities, and thereby shift protein pI, and then applied multiple methods to assess protein–AuNP assembly. At pH values lower than the anticipated pI of the modified protein, AuNP exposure elicits changes in the optical absorbance of the protein–NP solutions and other properties due to aggregate formation. Above the expected pI, however, protein–AuNP interaction is minimal, and both components remain isolated, presumably because both species are negatively charged. These data demonstrate that protein modification provides a powerful tool for modulating whether nanoparticle–protein interactions result in material aggregation. The results also underscore that naturally occurring protein modifications found in vivo may be critical in defining nanoparticle–protein corona compositions.     

Cosmological Parameters from Planck Data in SU(2)CMB,Their Local ΛCDM Values,and the Modified Photon Boltzmann Equation

A review of the spatially flat cosmological model SU(2)_CMB, minimally induced by the postulate that the cosmic microwave background (CMB) is subject to an SU(2) rather than a U(1) gauge principle, is given. Cosmological parameter values, which are determined from the Planck CMB power spectra at small angular scales, are compared to their values in spatially flat ΛCDM from both local and global extractions. As a global model SU(2)_CMB leans toward local ΛCDM cosmology and is in tension with some global ΛCDM parameter values. Spectral antiscreening / screening effects in SU(2)_CMB radiance are presented within the Rayleigh– Jeans regime in dependence on temperature and frequency. Such radiance anomalies can cause CMB large-angle anomalies. Therefore, it is pointed out how SU(2)_CMB modifies the Boltzmann equation  for the perturbations of the photon phase space distribution at low redshift and why this requires to the solve the ℓ-hierarchy on a comoving momentum grid (q-grid) for all z.