Apoptosis is an Early Event During Phthalocyanine Photodynamic Therapy-Induced Ablation of Chemically Induced Squamous Papillomas in Mouse Skin

Abstract— Photodynamic therapy (PDT) is a promising new modality to treat malignant neoplasms including superficial skin cancers. In our search for an ideal photosensitizer for PDT, Pc 4, a silicon phthalocyanine, has shown promising results both in in vitro assays and in implanted tumors. In this study we assessed the efficacy of Pc 4 PDT in the ablation of murine skin tumors; and the evidence for apoptosis during tumor ablation was also obtained. The Pc 4 was administered through tail vein injection to SENCAR mice bearing chemically induced squamous papillomas, and 24 h later the lesions were illuminated with an argon ion-pumped dye laser tuned at 675 nm for a total light dose of 135 J/cm². Within 72-96 h, almost complete tumor shrinkage occurred; no tumor regrowth was observed up to 90 days post-PDT. As evident by nucleosome-size DNA fragmentation, appearance of apoptotic bodies in hematoxylin and eosin staining and direct immunoperoxidase detection of digoxigenin-labeled genomic DNA in sections, apoptosis was clearly evident 6 h post-PDT at which time tumor shrinkage was less than 30%. The apoptotic bodies, as evident by the condensation of chromatin material around the periphery of the nucleus and increased vacuolization of the cytoplasm, were also observed in electron microscopic studies of the tumor tissues following Pc 4 PDT. The extent of apoptosis was greater at 15 h than at 6 and 10 h post-PDT. Taken together, our results clearly show that Pc 4 may be an effective photosensitizer for PDT of nonmelanoma skin cancer, and that apoptosis is an early event during this process.     

Assembly of Gold Nanoparticles on Gold Nanorods Using Functionalized Poly(N‐isopropylacrylamide) as Polymeric “Glue”

A telechelic thermoresponsive polymer, α‐amino‐ω‐thiol‐poly(N‐isopropylacrylamide) (H₂N‐PNiPAM‐SH), is used as the polymeric glue to assemble gold nanoparticles (AuNPs) around gold nanorods (AuNRs) into a satellite structure. Prepared by reversible addition‐fragmentation chain transfer polymerization followed by hydrazinolysis, H₂N‐PNiPAM‐SH is able to interlink the two types of the gold building blocks with the thiol‐end grafting on AuNRs and the amine‐end coordinating on the AuNP surface. The density of the grafted AuNPs on AuNRs can be tuned by adjusting the molar ratio between AuNPs and AuNRs in the feed. The resulted satellite‐like assembly exhibits unique optical property that was responsive to temperature change.     

Probing crystallinity of never-dried wood cellulose with Raman spectroscopy

The structure of wood cell wall cellulose in its native state remains poorly understood, limiting the progress of research and development in numerous areas, including plant science, biofuels, and nanocellulose based materials. It is generally believed that cellulose in cell wall microfibrils has both crystalline and amorphous regions. However, there is evidence that appears to be contrary to this assumption. Here we show, using 1064-nm FT-Raman spectroscopy, that (1) compared to the crystalline state, cellulose in the never-dried native state is laterally aggregated but in a less-than crystalline state wherein internal chains are water-accessible, (2) hydroxymethyl groups (CH₂OH) in cellulose exist not only in the tg conformation but also in the gt rotamer form, and (3) in native-state fibrils, low-frequency Raman bands due to cellulose crystal domains are absent, indicating the lack of crystallinity. Further evidence of the absence of crystallinity of the fibrils was the failure of the normal 64 % H₂SO₄ hydrolysis procedure to produce nanocellulose crystals from untreated wood. X-ray diffraction data obtained on wood, treated-wood, and wood-cellulose samples were consistent with the new finding and indicated that full-width-at-half-height of the X-ray diffractograms and lateral disorder in samples as measured by Raman were correlated (R² = 0.95).     

Iodination of industrially important aromatic compounds with aqueous potassium triiodide

A new reagent system consisting of aqueous KI₃ in AcOH and NaIO₄ as oxidant has been found to be effective in iodinating a variety of commercially important aromatic substrates under ambient conditions. The presence of Na₂SO₃ enhances the yield and the product purity. The procedure ensures high yields (72–98%) at room temperature in a short reaction time. A remarkable feature of this system is that even acidsensitive functionalities like anilines can be iodinated quantitatively.     

Quantitative Comparison of the Antimicrobial Efficiency of Leaching versus Nonleaching Polymer Materials

New antimicrobial materials will be more and more in the focus for hygienic and clinical disease control. Antimicrobial materials have to be distinguished in leaching and nonleaching materials. For many applications of antimicrobial materials on implants the use of nonleaching materials is essential. Therefore, the antimicrobial efficiency of leaching and nonleaching polymers has been investigated quantitatively in vitro in direct comparison on a highly relevant implant of central venous catheters (CVCs) using a well‐established called Certika test. This test is especially designed to test antimicrobial properties of leachable and nonleachable materials. This contribution demonstrates that newly developed nonleaching antimicrobial CVCs are equivalent to conventional leaching CVC systems in their antimicrobial performance against gram‐positive and gram‐negative bacteria, as well as Candida species. The use of new nonleaching antimicrobial polymers as shown here for CVCs represents a different mode of action with the aim to prevent infections also with antibiotic‐resistant strains and reduced side effects.

     

Variational relation problems in a general setting

In this paper, we consider variational relation problems involving a binary relation. The framework presented is more general than that in [J. Optim. Theory Appl. 138 (2008) , 65–76] and in other recent papers which deal with this subject.     

Compact standing wave thermoacoustic generator for power conversion applications

In the past decade, a variety of thermoacoustic engines (TAEs) were devised to convert thermal energy to acoustic power. In this paper, we optimized the design of a standing wave thermoacoustic generator that can provide high intensity acoustic pressure and convert it into electrical power output using a low cost alternator. Three prototypes of standing wave thermoacoustic generator (TAG) were designed to optimize the overall efficiency. The first prototype of standing wave TAG could produce an acoustic pressure of 0.9 kPa (153 dB) with an input thermal power of 210 W. Further, the maximum heat to electrical conversion efficiency was 0.045% with an input thermal power of 250 W. However, the performance of this system was not fully optimized. The performance of TAE depends upon various parameters including stack position, stack length and resonator length. Hence, a new second prototype of tunable TAG was developed to tune these critical parameters in order to improve the overall efficiency. A compact third prototype of TAG was successfully built with optimized parameters and has been tested. In the improved design, high intensity acoustic pressure of 2.9 kPa (163.5 dB) was observed for the same 210 W input thermal power. The maximum heat to electrical energy conversion efficiency was 0.084% with an input of 250 W which is 87% higher as compared to the first prototype. The major reason for the lower conversion efficiency is due to the low efficiency of the alternator. In future, high efficiency alternator designs can be employed along with careful impedance matching to obtain higher conversion efficiencies. The results described in this paper demonstrate the potential of developing compact portable acoustic power and electricity generators for decentralized power applications.     

Multiple Solutions of Generalized Multipoint Conjugate Boundary Value Problems

We consider the boundary value problem

Barium nitrate catalyzed one pot synthesis of 1,4‐dihydropyridines under solvent free conditions at room temperature

Barium nitrate acts as an efficient catalyst for the three‐component one pot synthesis of 1,4‐dihydropyridines. Barium nitrate is a safe chemical, and reaction without the use of organic solvents makes the process eco‐friendly.     

Can nature's design be improved upon? High strength, transparent nacre-like nanocomposites with double network of sacrificial cross links

The preparation of a high-strength and highly transparent nacre-like nanocomposite via layer-by-layer assembly technique from poly(vinyl alcohol) (PVA) and Na+-montmorillonite clay nanosheets is reported in this article. We show that a high density of weak bonding interactions between the polymer and the clay particles: hydrogen, dipole-induced dipole, and van der Waals undergoing break-reform deformations, can lead to high strength nanocomposites: sigmaUTS approximately 150 MPa and E' approximately 13 GPa. Further introduction of ionic bonds into the polymeric matrix creates a double network of sacrificial bonds which dramatically increases the mechanical properties: sigmaUTS approximately 320 MPa and E' approximately 60 GPa.