Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes

Rheumatoid arthritis (RA) is an autoimmune disease characterized by infiltration of immune cells in the synovium. However, the crosstalk of immune cells and synovial fibroblasts is still largely unknown. Here, global miRNA screening in plasma exosomes was carried out with a custom microarray (RA patients vs. healthy controls = 9:9). A total of 14 exosomal miRNAs were abnormally expressed in the RA patients. Then, downregulated expression of exosomal miR-204-5p was confirmed in both the replication (RA patients vs. healthy controls = 30:30) and validation groups (RA patients vs. healthy controls = 56:60). Similar to the findings obtained in humans, a decreased abundance of exosomal miR-204-5p was observed in mice with collagen-induced arthritis (CIA). Furthermore, Spearman correlation analysis indicated that plasma exosomal miR-204-5p expression was inversely correlated with disease parameters of RA patients, such as rheumatoid factor, erythrocyte sedimentation rate, and C-reactive protein. In vitro, our data showed that human T lymphocytes released exosomes containing large amounts of miR-204-5p, which can be transferred into synovial fibroblasts, inhibiting cell proliferation. Overexpression of miR-204-5p in synovial fibroblasts suppressed synovial fibroblast activation by targeting genes related to cell proliferation and invasion. In vivo assays found that administration of lentiviruses expressing miR-204-5p markedly alleviated the disease progression of the mice with CIA. Collectively, this study identified a novel RA-associated plasma exosomal miRNA-204-5p that mediates the communication between immune cells and synovial fibroblasts and can be used as a potential biomarker for RA diagnosis and treatment.Subject terms: Diagnostic markers, Non-coding RNAs     

第一个含桥基大环配体的CuⅡ-CoⅡ-CuⅡ异三核配合物的合成和结构

报道了结构新颖的异三核配合物[Co{Cu(μ-L>(N₃)₂}₂(H₂0)₂]·2H₂O的合成和晶体结构(H₂L:2,3-二羰基-1, 4, 8, 11-四氮杂十四烷).三核配合物由含有草酰胺外延桥基的大环铜(Ⅱ)配合物作为"配合物配体"与金属钴离子(Ⅱ)反应得到.晶体属单斜晶系,P2(l)/c空间群.a=0.77172(4) nm, b=1.531 30(3) nm, c=1.47298(7) nm,β=98.540(2)°, V=1.72137(13) nm³,Z=2,R=0.0843.晶体中包含三核配合物单元和溶剂水分子,并通过分子间氢键连成一维链状结构.     

Chemical composition and antioxidant activities of the essential oil from Nandina domestica fruits

The chemical composition and antioxidant activities of the essential oil from Nandina domestica fruits were studied for the first time. Twenty-two compounds, representing 82.79% of the oil, were identified from the oil. The major compounds were 3-hexen-1-ol (12.9%), linalool (12.3%), 2-methoxy-4-vinylphenol (9.9%), oleic acid (8.0%), furfural (5.8%) and 2,6-di-tert-butyl-4-methylphenol (5.7%). The antioxidant activities of the oil were evaluated using reducing power, metal chelating ability and scavenging capacity against 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis-3-ethylbenzthiazoline-6-sulfonate (ABTS) and superoxide anion free radical. The oil exhibited significant antioxidant activities.     

N-烷基-N-酰基磺酰胺聚苯乙烯基微球作为固相酰化剂的研究

一种可循环使用的固相试剂：N-烷基-N-酰基磺酰胺聚苯乙烯基微球(5), 通过对聚苯乙烯磺酰氯微球树脂进行两步功能基化的修饰反应来制备. 制备过程如下：聚苯磺酰氯树脂(1)与伯胺(2)反应得到聚苯乙烯基N-烷基磺酰胺树脂(3), 树脂3用酰氯(4)或酸酐酰化得到N-烷基-N-酰基磺酰胺聚苯乙烯基树脂(5). 酰化的树脂5作为酰基转移试剂与亲核试剂胺反应得到二级酰胺. 根据5上取代基对酰胺生成的程度的影响结果表明, 烷基R¹和酰基(R²CO)对酰基转移反应活性的大小依次分别为：苯基＞苄基＞甲基＞正丁基＞＞H和对硝基苯甲酰基(苯甲酰基＞乙酰基. 胺的亲核能力对酰胺的收率也有一定的影响. N-苯基-N-苯甲酰基磺酰胺树脂重复使用3次没有发现活性降低.     

PREPARATION OF MONODISPERSE CROSSLINKED POLYMER MICROSPHERES HAVING CHLOROMETHYL GROUP BY DISTILLATION-PRECIPITATION POLYMERIZATION

Monodisperse crosslinked poly(chloromethylstyrene-co-divinylbenzene) (poly(CMSt-co-DVB)) microspheres were prepared by distillation-precipitation copolymerization of chloromethylstyrene (CMSt) and divinylbenzene (DVB) in neat acetonitrile. The polymer particles had clean surfaces due to the absence of any added stabilizer. The size of the particles ranges from 2.59 μm to 3.19 μm and with mono-dispersity around 1.002-1.014. The effects of monomer feed in copolymerization on the microsphere formation were described. The polymer microspheres were characterized by SEM and chlorinity elemental analysis.     

黑土有机质含量野外高光谱预测模型

以田间原状黑土野外实测高光谱反射率为研究对象,分析黑土有机质的光谱响应波段,运用光谱分析方法提取光谱指数,建立基于反射光谱特征的黑土有机质高光谱预测模型。得出如下结论:(1)黑土反射光谱特征差异主要在小于1 250 nm的光谱范围,尤其是在小于1 100 nm的范围,随着有机质含量的变化,该波谱范围内黑土反射光谱特征呈现单/双谷现象,有机质是影响黑土反射光谱特征的决定因素。(2)有机质与黑土反射率倒数对数微分的相关系数最高,最高值在1 260 nm,达到-0.77(R2),相关系数高的波谱范围为750~1 260 nm。(3)基于黑土野外光谱反射率的有机质含量高光谱预测模型稳定性强,预测能力较好,能够用于黑土有机质含量野外速测。     

Densities and dynamic viscosities of the binary system (water + 1-hexyl-3-methylimidazolium bromide) at different temperatures

Densities and viscosities were measured for pure ionic liquid [C₆mim][Br] (1-hexyl-3-methylimidazolium bromide) and the binary system (water + [C₆mim][Br]) at 0.1 MPa and in the (293.15 to 333.15) K range. The excess molar volume and viscosity deviation were calculated and correlated by Redlich–Kister polynomial expansions. The fitting parameters and the standard deviations were determined.     

拟合曲率综合预测裂缝方法建立及其在陆西凹陷中的应用

从陆西凹陷的实际资料出发，分析了裂缝在油气勘探中的作用及不同类型裂缝的特征，研究了影响构造裂缝发育的因素，认为构造裂缝的发育受构造部位，孔隙度断层等多种因素的影响，探讨了裂缝发育程度与相关影响因素之间的定量关系，给出了拟合求曲率的方法，与最近断层距离的计算方法，建立了裂缝密度的定量化预测方法-拟合曲率综合预测裂缝方法和相应的评价标准，并对陆西凹陷的裂缝分布进行预测。     

Singlet Biradical Versus Triplet Biradical/Zwitterion Characteristics in Isomers of C6−C5−C6−C7−C6-Fused Pentacyclic Aromatic Hydrocarbons Revealed through Reactivity Patterns

Among various polycyclic aromatic hydrocarbons, C₆−C₅−C₆−C₇−C₆ fused pentacyclic aromatic hydrocarbons have the unique potential to adopt quinonoid, zwitterion, singlet, or triplet biradical electronic configurations. Two such hybrid structures between pentacene and azulene were synthesized and their ground state electronic configurations were deduced from the reactivity patterns they exhibit respectively. Compound 6 , where the radicaloid carbons are linked through a para-phenylene, forms a head-to-head dimer like a singlet biradical. In contrast, isomer 7 , where the para-linkage was switched to meta, reacts readily with oxygen which resembles the reactivity of a triplet state. The oxidized intermediate(s) then undergoes rearrangement to furnish the C₆−C₅−C₆−C₆−C₆ ring contraction product 13 . Cation 14 , the protonated form of 7 , was synthesized, which implies 7 also reacts like a zwitterion. It was revealed the oxidative rearrangement takes place even with mesityl dibenzotropylium cation despite its perceived aromaticity. DFT calculations confirm the most stable forms of 6 and 7 are singlet and triplet diradical, which is consistent with the observed reactivity of respective molecules.     

In situ NMR diffusion coefficients assessment of lithium ion conductor using electrochemical priors and Arrhenius constraint——A computational study

In situ NMR measurements of the diffusion coefficients,including an estimate of signal strength,of lithium ion conductor using diffusion-weighting pulse sequence are performed in this study.A cascade bilinear model is proposed to estimate the diffusion sensitivity factors of pulsed-field gradient using prior information of the electrochemical performance and Arrhenius constraint.The model postulates that the active lithium nuclei participating electrochemical reaction are relevant to the NMR signal intensity,when discharge rate or temperature condition is varying.The electrochemical data and the NMR signal strength show a highly fit with the proposed model according our simulation and experiments.Furthermore,the diffusion time is constrained by temperature based on Arrhenius equation of reaction rates dependence.An experimental calculation of Li_4Ti_5O_(12)(LTO)/carbon nanotubes(CNTs) with the electrolyte evaluating at 20 ℃ is presented,which the b factor is estimated by the discharge rate.