Proteolysis in microfluidic droplets: an approach to interface protein separation and peptide mass spectrometry

A versatile microreactor protocol based on microfluidic droplets has been developed for on-line protein digestion. Proteins separated by liquid chromatography are fractionated in water-in-oil droplets and digested in sequence. The microfluidic reactor acts also as an electrospray ionization emitter for mass spectrometry analysis of the peptides produced in the individual droplets. Each droplet is an enzymatic micro-reaction unit with efficient proteolysis due to rapid mixing, enhanced mass transfer and automated handling. This droplet approach eliminates sample loss, cross-contamination, non-specific absorption and memory effect. A protein mixture was successfully identified using the droplet-based micro-reactor as interface between reverse phase liquid chromatography and mass spectrometry.     

An investigation of teachers’ intentions and reflections about using Standards-based and traditional textbooks in the classroom

This study analyzed teachers’ intentions for and reflections on their use of Standards-based [Connected Mathematics Program (CMP)] textbooks and traditional (non-CMP) mathematics textbooks to guide instruction. In this investigation of the interplay between textbooks and instruction, we focused on learning goals, instructional tasks, teachers’ anticipation of students’ difficulties, and their perceptions of students’ achievement of learning goals. All of these are aspects of teachers’ intentions and reflections that have proved fruitful in comparing the roles of the CMP and non-CMP mathematics textbooks in our Longitudinal Investigation of the Effect of Curriculum on Algebra Learning project. Whereas the cognitive level of the teachers’ intended learning goals appeared generally to reflect the emphases of their respective textbooks, we found that the CMP teachers’ intended learning goals were not as well aligned with the CMP textbooks as the non-CMP teachers’ learning goals were aligned with their non-CMP textbooks. The CMP and non-CMP teachers’ implementations of the lessons seemed to reduce the degree of difference between the cognitive levels of their intended goals. Even so, we found that significantly more CMP lessons than non-CMP lessons were implemented at a high level of cognitive demand. Although the non-CMP teachers’ intended learning goals were better aligned with their textbook’s learning goals, we found that the CMP teachers were more likely than the non-CMP teachers to follow the guidance of their textbooks in designing and selecting instructional tasks for a lesson. Future research should consider other aspects of teachers’ intentions and reflections that may shed a broader light on the role of textbooks and curriculum materials in teachers’ crafting of instructional experiences for their students.     

Estimating returns to scale of Chinese airport airside activities using the CCR-0-objective RTS method

The returns to scale (RTS) nature of 41 Chinese airport airsides is investigated in this paper. We introduce take-off distance available and landing distance available into RTS estimation. To deal with the existence of multiple optimal solutions when estimating RTS using the CCR (Charnes, Cooper and Rhodes) RTS method in Data Envelopment Analysis, we introduce the CCR-0-objective RTS method. The empirical study shows that all those airsides with two runway operate under decreasing RTS. Those airsides with only one runway either show constant RTS or show increasing RTS.     

A note on dynamic Stackelberg games with leaders in turn

Recently, a model of dynamic Stackelberg games with leaders in turn has been proposed, and dynamic Stackelberg games with leaders in turn have been exploited under a feedback information structure. This paper characterizes dynamic Stackelberg games with leaders in turn under other information structures, both closed-loop and open-loop information structures. Explicit solutions are given for linear-quadratic systems under an open-loop information structure for dynamic Stackelberg games with leaders in turn.     

Controlled Dense Coding Through a Genuine Five-Atom Entangled State in Cavity QED

We present an experimentally feasible scheme for implementing controlled dense coding through a genuine five-atom entangled state in cavity QED. The scheme is insensitive to both the cavity decay and the thermal field. In the scheme that four-atom entangled states can be exactly distinguished with detecting the atomic state, and the controlled dense coding can be realized in a simple way.     

Analysis of the electrically forced vibrations of piezoelectric mesa resonators

We study the electrically forced thickness-shear and thickness-twist vibrations of stepped thickness piezoelectric plate mesa resonators made of polarized ceramics or 6-ram class crystals. A theoretical analysis based on the theory of piezoelectricity is performed, and an analytical solution is obtained using the trigonometric series. The electrical admittance, resonant frequencies, and mode shapes are calculated, and strong energy trapping of the modes is observed. Their dependence on the geometric parameters of the resonator is also examined.     

The electric and magnetic properties of epitaxially grown A0.5-xLaxSr0.5MnO3 （A=Pr, Nd） thin film

The epitaxial （single crystal-like） Pr0.4La0.1Sr0.5MnO3 （PLSMO） and Nd0.35La0.15Sr0.5MnO3 （NLSMO） thin films are prepared and characterized, and the electric and magnetic properties are examined. We find that both PLSMO and NLSMO have their own optimum deposition temperature （To） in their growing into epitaxial thin films. When the deposition temperature is higher than To, a c-axis oriented but polycrystalline thin film grows; when the deposition temperature is lower than To, the thin film tends to be a-axis oriented and also polycrystalline. The most important point is that for the epitaxial PLSMO and NLSMO thin films the electronic phase transitions are closely consistent with the magnetic phase transitions, i.e. an antiferromagnetic phase corresponds to an insulating state, a ferromagnetic phase corresponds to a metallic state and a paramagnetic phase corresponds to a semiconducting state, while for the polycrystalline thin films the electronic phase transitions are always not consistent with the magnetic transitions.     

Effect of Co layer thickness on structural and magnetic properties of C/Co/C films

Granular C/Co/C films have been prepared by magnetron sputtering from C and Co onto glass substrates at room temperature and subsequent in situ annealing. It has been found that the structure and magnetic properties of the C/Co/C films depend strongly on the Co layer thickness. Vibrating sample magnetometer measurements indicate that the in-plane coercivities reach maximum in 20 nm Co thickness of both as-deposited and annealed films. The squareness ratio of annealed films was more than 0.8. X-ray diffraction shows that majority Co nanograins are formed as the hexagonal-close-packed (HCP) structure in 20 nm Co thickness with annealing at 400 °C. Scanning probe microscope was used to scan surface morphology and magnetic domain structures. The values of the surface roughness were lower than 0.6 nm in all annealed samples. The average magnetic cluster size was estimated to be about 10 nm in annealed 20 nm Co thickness films.     

荧光俘获效应对掺饵氧化物玻璃光谱性质的影响

测试了不同掺杂浓度和样品厚度下掺铒磷酸盐和碲酸盐玻璃的吸收光谱、荧光光谱和荧光寿 命，计算了Er³⁺离子在1.53 μm处的吸收截面(σ_a)、发射截面(σ _e)、自发辐射跃迁概率(A_rad)、辐射跃迁寿命(τ_rad) 、以及辐射跃迁量子效率(η)等光谱参数.讨论了荧光俘获效应对掺铒磷酸盐和碲酸盐玻璃 光谱性质及光谱参数的影响.结果表明即使在铒离子低掺杂浓度(0.1 mol% Er₂O ₃)下，荧光俘获效应也普遍存在于掺铒玻璃材料中，使得荧光寿命(τ_{f)和荧光半高宽(FWHM)随样品的厚度和铒离子掺杂浓度增加而增大，导致碲酸盐和磷酸 盐玻璃中τf分别增加11%—37％和6%—17%，FWHM分别增加15%—64%和11%—55% ，使得掺铒玻璃材料的放大品性参数(σe×FWHM) 也相应被估高.由于铒离子在 碲酸盐玻璃中在1.53 μm处吸收和发射截面重叠面积较大，加之铒离子在前者基质中的发射 截面高于后者，使得掺铒碲酸盐玻璃中的荧光俘获效应高于磷酸盐玻璃. 关键词： 荧光俘获 铒离子 碲酸盐玻璃 磷酸盐玻璃}     

Eu3+掺杂Bi2O3-TeO2-B2O3-ZnO玻璃光谱性质

测量了Eu3+（1 mol%）掺杂(60-χ)Bi2O3-χ TeO2-30B2O3-10ZnO（χ＝5,10,20,30,摩尔百分比）玻璃的吸收光谱、发射光谱、激发光谱以及声子边带谱.根据稀土离子Eu3+光学跃起矩阵元的特点,从发射光谱获得了Eu3+光学跃起的J-O参数Ω2与Ω4.结果显示,强度参量Ω2随着Bi2O3量的增加与TeO2量的减少而减小,表明材料的对称性提高, Eu-O键强减弱,共价性减弱.随着Bi2O3量的增加,电－声子偶合减弱,材料的热稳定性大幅度提高.