Approaches to unsaturated analogues of nucleosides comprising four- and six-membered rings

Unsaturated nucleoside analogues 21, 22, 46, and 54, comprising four- and six-membered rings, were synthesized using two different approaches. The 2-benzyloxycycloalkanones 23a and 23b served as starting materials for both methods. Conversion to methylenecyclobutanes 29a and 29b was followed by addition of bromine via pyridinium perbromide to give vicinal dibromides 30a and 30b. Reaction of 29a with Br2 gave a ring-contracted cyclopropane derivative 31. Alkylation-elimination of adenine with 30a gave bromoalkene 32 as the major product and adenine-containing unsaturated derivatives 33, 34, and 35 as minor components. Vicinal dibromide 30b gave the Zaitsev cyclohexene 45 as the only product. Epoxidation of 29a and 29b afforded oxiranes 36a and 36b which were used in alkylation of adenine to furnish hydroxy derivatives 37a, 37b, 38a, and 38b. Beta-elimination via mesylates 39a and 40a using tBuOK/DMF gave Z- and E-methylenecyclobutanes 34 and 35. With an excess of base the E-bis-methylenecyclobutane 41 was obtained. Mesylation of cyclohexane derivatives 37b and 38b gave the Z- and E-N6-mesylated product 48. By contrast, the N6-benzoyl derivatives 49 and 50 afforded O-mesyl intermediates 51 and 52. Beta-elimination gave both Hofmann and Zaitsev products 53 and 45. O-Debenzylation of 34 and 35, 45, and 53 afforded analogues 21, 22, 46, and 54. The E-isomer 22 was also obtained by hydroboration procedure from E-bis-methylenecyclobutane 41.     

Elucidation of stabilizing pickering emulsion with jackfruit filum pectin-soy protein nanoparticles obtained by photocatalysis

There is still the dearth of reports of jackfruit filum pectin-based nanoparticles as the Pickering emulsifiers with respect to the applications in foods, cosmetics and medicines. So we fabricated soy protein-jackfruit filum pectin nanoparticles (SPP) by photocatalysis as Pickering emulsifier. Jackfruit filum pectin exhibited lower yield (17.31%), degree of methoxylation (15.53%), but higher galacuronic acid content (74.22%). A strong linkage between pectin and soy protein was formed by photocatalysis. The conjugated polymer could self-assemble into compact near globular nanoparticle. The mean size of SPP was larger than that of soy protein nanoparticles but smaller than that of soy protein-pectin complex without photocatalysis. Besides, the zeta potential of SPP was ?33.8?mV, significantly lower than that of soy protein nanoparticles but higher than that of control sample, further confirming that SPP surfaces were completely covered with pectin molecules. Compared with control sample, the three-phase contact angle increased from 42.7 to 90.6°, indicated that SPP could be developed as effective Pickering emulsifiers. The emulsions stabilized by SPP exhibited high thermal stability and excellent salt tolerance as well as good freeze-thaw stability in comparison with emulsions covered with control sample. These findings would provide a potential way of producing effective Pickering emulsifier.GRAPHICAL ABSTRACT     

矩形截面毛细管电泳和二维分离

在石英单晶表面制成矩矩截面毛细管柱中进行电泳实验。由于矩形柱比圆形柱有更大散热侧面积且石英单晶的导热性能远无于熔融石英，所以可施加较高的场强，不仅提高了柱效，而且缩矩了分离时间。两相交的通道之间形成自然连接，可实现二维分离，并消除死体积。     

Notable shift of ultraviolet intensity on Sn-doped ZnO nanostructure fabricated by sol–gel method

Sn-doped ZnO (SZO) thin films are deposited by sol–gel dip-coating method with Sn content at 0 at.% and 1–15 at.% with an increment of 2 at.%. The structure and luminescence of the films are investigated. X-ray diffraction results indicate that all the SZO samples show preferential orientation along the (002) direction, and the scanning electron microscope exhibits that the surface morphology of the films change from nanoparticles to nanorods with increasing Sn concentration. X-ray photoelectron spectroscopy reveals that Sn exists as valence of +4 in the matrix. The photoluminescence peaks at 381 and 398 nm are observed in all the samples. The ratio of intensity of peak at 381 nm to that of peak at 398 nm differed markedly. The intensity of peak at 398 nm might be due to the response for the Sn atoms, while the intensity of peak at 381 nm is probably related to the quantum size effect.     

The intersection marker method for 3D interface tracking of deformable surfaces in finite volumes

Currently, the majority of computational fluid dynamics (CFD) codes use the finite volume method to spatially discretise the computational domain, sometimes as an array of cubic control volumes. The Finite volume method works well with single‐phase flow simulations, but two‐phase flow simulations are more challenging because of the need to track the surface interface traversing and deforming within the 3D grid. Surface area and volume fraction details of each interface cell must be accurately accounted for, in order to calculate for the momentum exchange and rates of heat and mass transfer across the interface. To attain a higher accuracy in two‐phase flow CFD calculations, the intersection marker (ISM) method is developed. The ISM method is a hybrid Lagrangian–Eulerian front‐tracking algorithm that can model an arbitrary 3D surface within an array of cubic control volumes. The ISM method has a cell‐by‐cell remeshing capability that is volume conservative and is suitable for the tracking of complex interface deformation in transient two‐phase CFD simulations. Copyright © 2015 John Wiley & Sons, Ltd.     

非低温法的稀有气体纯化技术概述

稀有气体广泛应用于工业生产中,如离子刻蚀、检漏等。但是它们在地球上的含量比较少,属于稀有资源,因此需要对它们进行纯化以循环再利用。首先介绍了不同气体纯化的原理,然后以实际生产中的不同稀有气体使用范围为例,说明纯化装置的设计方法和需要考虑的问题。为以后气体纯化装置的设计提供借鉴。     

DNA Degradation of Genetically Modified Cottonseed Meal During Feed Processing

The effects of dry heating, wet heating, and extrusion on the degradation of DNA in cottonseed meal (CSM) were studied using polymerase chain reaction (PCR) and real-time PCR approach. Both the sad1 DNA, ranging between 128 and 883 bp in size, and the cry1Ab/c gene, ranging between 183 and 652 bp in size, were detectable in all dry-heated CSM and cottonseed. During wet heating, the sad1 gene (≥883 bp) and the cry1Ab/c (≥952 bp) gene were thoroughly degraded at 105 and 120 °C, respectively. Sizes from 128 to 530 bp for the sad1 gene and sizes from 183 to 652 bp for the cry1Ab/c gene were detected during extrusion at temperatures ranging from 75 to 135 °C. Fragments ≤883 bp for the sad1 gene and ≤952 bp for the cry1Ab/c gene were detected in all of the extruded samples with water content varying between 26 and 34 %. The copy number ratio of cry1Ab/c to sad1 in samples of Bt cottonseed meal decreased rapidly when the temperature increased during the heating process. In conclusion, feed processing markedly degrades the larger DNA fragments of sad1 and cry1Ab/c, with high temperature and water content being the main factors for that degradation.     

Chemical Constituents of the Roots of Phlomis betonicoides and Their Anti-Rotavirus Activity

Chemistry of Natural Compounds - Two new isobenzofuranone derivatives (1 and 2) together with six known compounds (3–8) were isolated from the roots of Phlomis betonicoides, a highly sweet...