The Pharmacokinetics in Mice and Cell Uptake of Thymus Immunosuppressive Pentapeptide Using LC-MS/MS Analysis

Thymus immunosuppressive pentapeptide (TIPP) is a novel anti-inflammatory peptide with high efficacy and low toxicity. This study aims to establish a selective LC-MS/MS method for analyzing the analyte TIPP in biological samples, laying the foundation for further PK and PD studies of TIPP. Protein precipitation was conducted in acetonitrile supplemented with 2% formic acid and 25 mg/mL dithiothreitol as a stabilizer, which was followed by backwashing the organic phase using dichloromethane. The chromatographic separation of TIPP was achieved on a C18 column with a gradient elution method. During positive electrospray ionization, TIPP was analyzed via multiple-reaction monitoring. The linear relationships between the concentration of TIPP and peak area in murine plasma cell lysates, supernatants, and the final cell rinse PBS were established within the ranges of 20–5000 ng/mL, 1–200 ng/mL, 10–200 μg/mL, and 0.1–20 ng/mL, respectively (r² > 0.99). Validated according to U.S. FDA guidelines, the proposed method was proved to be acceptable. Such a method had been successfully applied to investigate the pharmacokinetics of TIPP in mice via subcutaneous injection. The plasma half-life in mice was 5.987 ± 1.824 min, suggesting that TIPP is swiftly eliminated in vivo. The amount of TIPP uptake by RBL-2H3 cells was determined using this method, which was also visually verified by confocal. Furthermore, the effective intracellular concentration of TIPP was deduced by comparing the intracellular concentration of TIPP and degrees of inflammation, enlightening further investigation on the intracellular target and mechanism of TIPP.     

辉光等离子体辅助化学气相沉积低温合成金刚石薄膜的研究

采用辉光放电等离子体增强化学气相沉积 (GP CVD)技术在低温条件下合成了高品质的亚微米金刚石薄膜 ,并通过对合成过程的实时发射光谱诊断确定了 [CH4 H2 ]系统参与金刚石合成反应的主要荷能粒子。对合成过程的研究表明 :采用这种技术能使电子增强热丝化学气相沉积 (EACVD)合成高品质金刚石薄膜的温度从 85 0℃降至 (340± 5 )℃ ;薄膜低温合成中的主要荷能粒子为CH3 、CH ,CH+ 、H 等 ,其中过饱和原子氢保证了高品质金刚石薄膜的合成 ;根据光诊断和探针测量的结果推断近表面辉光放电可在基片表面形成电偶极层 ,该偶极层是进行超常态反应的必要环境 ,并在低温合成中起重要作用     

Theoretical analysis on double Rayleigh backscattering noise in optical fibreRaman amplifiers and its suppression

In this paper, amplified double Rayleigh backscattering noise (DRB) in the optical fibre Raman amplifier is analysed. Expressions are presented for both forward pumping and backward pumping schemes. Calculation is performed to show the effective suppression of DRB noise by employing an optical isolator. The optimal position for the isolator is determined and is found to be insensitive to the power levels of the signals and pumps. The results show that a reduction of the DRB noise by almost 2 to 3 orders can be reached.     

基于数学形态学的表面原子熔融相的 STM图像识别算法

高温扫描隧道显微镜(HT-STM)可以实时原位地捕捉到表面原子的熔化相变过程.在这一原位变温实验中,快速可靠地识别出每帧STM图像中的熔融相十分关键.传统的手工统计方法存在效率低下、随意性大等问题.我们发展出一套基于数学形态学的算法,来自动快速地识别.与人工方法相比,该算法消除了人为主观误差,使确定的边界更加准确、光滑,处理效率提高了266倍.     

β-胡萝卜素分子的黄琨因子的温度特性

β-胡萝卜素具有光采集、光防护功能, 又是重要的光电材料, 它在外场下的分子结构和性能变化既有理论意义也有应用价值。测量了β-胡萝卜素在环己醇中68～26 ℃温度范围内的紫外-可见吸收、拉曼光谱。实验结果表明随着温度的降低, 黄琨因子和碳碳键每个振动模的电子-声子耦合常数减小, 紫外-可见吸收光谱红移, 碳碳键拉曼散射截面增加。用线性链状多烯分子的“相干弱阻尼电子-晶格振动模型”、“有效共轭长度模型”等理论给予了解释。随着温度的降低,β-胡萝卜素分子的热无序减小,分子结构有序性增加,π电子离域扩展,有效共轭长度增加,导致紫外-可见吸收光谱红移和强的拉曼活性。相干弱阻尼电子-晶格振动增强,使碳碳键拉曼散射截面增加。引用带有量纲的电子-声子相互作用常数,既可以与黄昆因子建立关系式,计算出碳碳键每个振动模的数值,也可以表征分子的有效共轭长度,π电子离域程度及拉曼散射截面的大小等。     

一种改进的夜间数字能见度测量方法

为降低背景光噪声对数字摄像法夜间能见度测量的不确定性影响,对双光源法能见度测量系统进行改进。该系统利用避光筒限制CCD相机视场角,拍摄2个位于不同距离的LED背光源,然后对摄取到的图像进行处理得到光源图像的灰度值,再根据阿拉德定律求得大气消光系数,计算出大气能见度值。通过在无背景光、有背景光未加避光筒和加避光筒3种条件下的比对实验,分析光源图像的灰度信息,并将实验结果进行处理,得出有避光筒和无避光筒时测得的能见度值与无背景光下测量结果的相关系数分别是0.911 3和0.322 7,均方根相对偏差分别是6.87% 和23.38%。结果表明:系统抑制背景光噪声的能力明显增强,能较准确地测量夜间能见度。     

The influences of amplified spontaneous emission,crystal temperature and round-trip loss on scaling of CW thin-disk laser

In this paper, a model is built to explore how the parameters (amplified spontaneous emission (ASE), temperature and round-trip loss) influence the output power in a thin-disk laser. It is found that optical efficiency of the disk laser is reduced with the increase of ASE, temperature or round-trip loss. The parameters are optimized to maximize the output power based on our model. We find that it is necessary to balance the need to lower the temperature with the need to control ASE during the optimization process. But the balance becomes more difficult to achieve with the increase of round-trip loss. We conclude that output power of more than 2.6 MW with a single disk can be achieved, but the necessary disk size (more than 0.5 m) is far beyond the actual technical limits. But it is possible to achieve output power of over a hundred kilowatts using a 10 cm disk in the near future.     

The invariant of the stiffness filter function with the weight filter function of the power function form

Based on the independent, continuous and mapping (ICM) method and homogenization method, a research model is constructed to propose and deduce a theorem and corollary from the invariant between the weight filter function and the corresponding stiffness filter function of the form of power function. The efficiency in searching for optimum solution will be raised via the choice of rational filter functions, so the above mentioned results are very important to the further study of structural topology optimization.     

一类非光滑对称三次系统的全局分支

该文对一类对称三次Hamilton系统在非光滑对称摄动下产生的极限环数目进行研究.通过多参数摄动理论和定性分析方法,得到这类在非光滑摄动下的三次系统可以存在至少19个极限环.