Release after transmission: Arginine-rich, cell-penetrating peptides (CPPs) mediate cytoplasmic delivery of trimethoprim (TMP)-terbium complex conjugates and selective, intracellular labeling of E.?coli dihydrofolate reductase (eDHFR) fusion proteins. A disulfide bond linking CPP and cargo is reduced following uptake. CPP conjugation can be used to deliver otherwise cell-impermeable, ligand-fluorophore conjugates. 相似文献
Brilliance of terbium : Heterodimeric conjugates of trimethoprim covalently linked to sensitized terbium chelates bind to Escherichia coli dihydrofolate reductase fusion proteins with nanomolar affinity (see picture). Terbium luminescence enables sensitive and time‐resolved detection of labeled proteins in vitro and on the surface of living mammalian cells.
The proton transfer compound LH2 , (phenH+)2(pydc2—), has been prepared from 1, 10‐phenanthroline, phen, and 2, 6‐pyridinedicarboxylic acid, (dipicolinic acid), pydcH2. Characterization was performed using solution and solid phase CP/MAS 13C NMR and IR spectroscopy. The reactions of this adduct with ZnSO4·7H2O and Zn(NO3)2·4H2O give the complexes, [Zn(pydc)2][Zn(phen)2(H2O)2]·7H2O (1) and [Zn(phen)3]4(H(Hpydc)2)(NO3)7·26H2O (2) , respectively. These complexes were characterized by 1H and 13C NMR spectroscopy and single crystal X‐ray analysis. The complexes crystallize in the triclinic space group P1 with Z = 2. The unit cell dimensions for complex 1 and 2 are: a = 9.9838(9) Å, b = 14.7483(13) Å, c = 14.8365(13) Å and a = 12.640(4) Å, b = 15.855(5) Å, c = 21.830(7) Å, respectively. In complex 1 (pydc2—) and phen, are tri‐ and bidentate ligands, respectively, and an anionic [Zn(pydc)2]2— and cationic [Zn(phen)2(H2O)2]2+ complex are formed simultaneously. In complex 2 , three phen participate in complexation leaving hydrogen‐bis(pyridine‐2‐carboxylate), (H(Hpydc)2)— as a supramolecular anion. The fragments (H(Hpydc)2)—, 7 NO3—, and 26 H2O in complex 2 are joined together by extensive and strong H‐bonding; therefore, the structure is composed of [Zn(phen)3]48+, and an anionic hydrogen bond supramolecular assembly with the formula, {(H(Hpydc)2(NO3)7)8— · 26H2O}n. The anionic species (H(Hpydc)2)— has a special position at the inversion center, as well as one of the NO3— anions, which is disordered over the inversion center. Most of the hydrogen bonds in complex 2 represent strong H‐bonding. The protonation constants of the building blocks of the pydc‐phen adduct, the equilibrium constants for the reaction of (pydc2—) with phenanthroline and the stoichiometry and stability of the ZnII complex with LH2 on aqueous solution were determined by potentiometric pH titration. The solution study results support self‐association between (pydc2—) and (phenH+) with a stoichiometry for the Zn(II) complex similar to that observed for the isolated crystalline complex. 相似文献
Visible light‐driven Al‐doped TiO2 with different aluminum contents (2, 5 and 10 mol%) were synthesized via a facile sol–gel method. Fourier transform infrared (FTIR), UV‐visible diffuse reflectance, energy dispersive X‐ray (EDX) spectroscopy as well as X‐ray diffraction (XRD), X‐ray fluorescence (XRF) and scanning electron microscopy (SEM) methods were used for the characterization of the obtained nanoparticles. The photocatalytic performance of the samples was evaluated by the degradation of rhodamine B (RhB) under visible light irradiation. The yield of the degradation RhB was estimated to be 71%, 89%, 65% and 56%, for the bare TiO2, 2%, 5% and 10% Al‐doped TiO2, respectively. It was found that 2 mol% of Al‐doped TiO2 shows the best photocatalytic performance. In low concentration of dopant, separation of photogenerated electron–hole pairs promoted, and subsequently, the degradation efficiency increased. It was proposed that the degradation of RhB by 2 mol% Al‐doped TiO2 photocatalyst follows both N‐deethylation and chromophore cleavage mechanisms, while the N‐deethylation still predominated over cleavage of dye chromophore structure. The key role of hydroxyl radicals in RhB degradation was verified by the effects of scavengers. In addition, the photocatalyst can be reused for three runs without any significant loss of its catalytic activity. 相似文献
Tandem reaction between acyl isoselenocyanates, generated from acyl chlorides and KSeCN, and benzene‐1,2‐diamine in acetone at room temperature, gave 1,3,5‐triazepineselone derivatives in moderate to good yields. 相似文献
Microchimica Acta - Copper nanoclusters (CuNCs) are an attractive alternative to other metal nanoclusters. The synthesis of CuNCs is highly efficient and fast, with low-cost and without any... 相似文献
Journal of Solid State Electrochemistry - A non-enzymatic sensor was developed for simultaneous detection of glucose, ethanol, and glycerol concentrations in wine using nickel nanoparticles on... 相似文献