High Solids Enzymatic Hydrolysis of Pretreated Lignocellulosic Materials with a Powerful Stirrer Concept

In this study, we present a powerful stirred tank reactor system that can efficiently hydrolyse lignocellulosic material at high solid content to produce hydrolysates with glucose concentration > 100 g/kg. As lignocellulosic substrates alkaline-pretreated wheat straw and organosolv-pretreated beech wood were used. The developed vertical reactor was equipped with a segmented helical stirrer, which was specially designed for high biomass hydrolysis. The stirrer was characterised according to mixing behaviour and power input. To minimise the cellulase dosage, a response surface plan was used. With the empirical relationship between glucose yield, cellulase loading and solid content, the minimal cellulase dosage was calculated to reach at least 70 % yield at high glucose and high substrate concentrations within 48 h. The optimisation resulted in a minimal enzyme dosage of 30 FPU/g dry matter (DM) for the hydrolysis of wheat straw and 20 FPU/g DM for the hydrolysis of beech wood. By transferring the hydrolysis reaction from shaking flasks to the stirred tank reactor, the glucose yields could be increased. Using the developed stirred tank reactor system, alkaline-pretreated wheat straw could be converted to 110 g/kg glucose (76 %) at a solid content of 20 % (w/w) after 48 h. Organosolv-pretreated beech wood could be efficiently hydrolysed even at 30 % (w/w) DM, giving 150 g/kg glucose (72 %).     

40 GHz mode-beating with 8 Hz linewidth and 64 fs timing jitter from a synchronized mode-locked quantum-dash laser diode

Linewidth narrowing of the radio frequency beat-tones and the optical-modes is experimentally investigated in a ～40 GHz quantum-dash mode-locked laser diode subject to optical injection of 10 GHz pulses. In comparison to the 75 kHz linewidth exhibited by the beat-tones in passive mode-locking conditions, a remarkable reduction to less than 8 Hz is achieved when the laser is under optical injection. From this beat-tone signal, an integrated root-mean-square timing jitter of 64 fs is calculated. In addition, a quadratic profile of the optical linewidth with the wavelength is observed in active locking, reaching a minimum of 1.7 MHz for the longitudinal modes around 1530 nm and progressively increasing to 37.4 MHz for modes at 1525 nm.     

Die Titration der Zuckerarten

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Zur mafsanalytischen Bestimmung der Ferro- und Ferricyanwasserstoffs?ure

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