Experimental investigation of the CMN matrix element in the band anticrossing model for GaAsN and GaInAsN layers

The temperature dependence of bandgap energy of GaAs_0.98N_0.02 and Ga_0.95In_0.05As_0.98N_0.02 compounds has been analysed in order to determine the C_MN matrix element of the band anticrossing model. We have found that the element equals 2.48 and 2.60 eV for GaAs_0.98N_0.02 and Ga_0.95In_0.05As_0.98N_0.02 layers, respectively. When the same value has been assumed for annealed layers, an increase in the energy of the resonant nitrogen level E_N has been obtained. Two possible mechanisms leading to the increase in this energy are discussed in this work.     

In situ STM study of potential-driven transitions in the film of a cationic surfactant adsorbed on a Au(111) electrode surface

Electrochemical scanning tunneling microscopy (EC-STM) has been employed to study the structure of a film formed by cationic surfactant N-decyl-N,N,N-trimethylammonium triflate (DeTATf) adsorbed on the Au(111) electrode surface. The film is disordered at potentials corresponding to either large negative charge densities or to positive charge densities. At small negative charge densities, an ordered adlayer of flat-lying DeTATf molecules is formed. High-resolution images of this adlayer reveal that the triflate anion is coadsorbed with the N-decyl-N,N,N-trimethylammonium cation, effectively forming an ion pair at the electrode surface. This is a significant result because it explains why this surfactant behaves like a zwitterionic surfactant at the metal/solution interface.     

Polyketanils. Polymers protonated with Bronsted acid

New luminescent materials—aromatic polyketanils (PKs) containing p‐phenylene and biphenylene linkages in the backbone and electron‐donating, side‐group substitutions—were synthesized via melt polycondensation with the goal of studying the interactions between the polymer chains (PKs), primary dopant [1,2‐(di‐2‐ethylhexyl)ester of 4‐sulfophthalic acid (PSA)], and secondary dopant [m‐cresol (MC)] with ¹H NMR, ¹³C NMR, Fourier transform infrared (FTIR), ultraviolet–visible, and photoluminescence measurements. Spectral changes were observed for the PKs after primary and secondary doping, and they supported ionic‐ and hydrogen‐bond formation between the PKs and the PSA and MC, respectively. This specific interaction of the dopant with the host polymer influenced the PK properties, and the following observations were made: (1) HOMO/LUMO energy gap of the protonated PKs; (2) bathochromic/hypsochromic photoluminescence shifts observed after protonation with PSA; (3) a downfield chemical shift of the labile proton in the protonating agent OH group in the ¹H NMR spectra of the protonated polymers; (4) a protonation decrease in the intensity of the C?N stretching band in the FTIR spectrum; and (5) a spherulite‐like morphology, detected by atomic force microscopy and polarized optical microscopy. © 2006 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 44: 5645–5660, 2006     

Investigations on aromatic polyesters with polynaphthalene systems in the main chain—IX. Influence of the chain structure of aromatic copolyesters on their glass transition temperatures

Glass transition temperatures of terephthalic copolyesters of 4,4′dihydroxydinaphthyl 1,1′ and 2,2 bis(4-hydroxyphenyl)propane with various mole ratios of the comonomers and different chain structures have been measured. A relationship has been found between T_g and the chain structure of aromatic copolyesters with polynaphthalene rings in the chain.     

Preparation and characterization of a polyimide nanofoam through grafting of labile poly(propylene glycol) oligomer

Preparation of a polyimide nanofoam (PI‐F) for microelectronic applications was carried out using a polyimide precursor synthesized from poly[(amic acid)‐co‐(amic ester)] and grafted with a labile poly(propylene glycol) (PPG) oligomer. Polyimide precursor was synthesized by partial esterification of poly(amic acid) (PAA) derived from pyromellitic dianhydride (PMDA) and 4,4′‐oxydianiline (ODA). The precursor was then grafted with bromide‐terminated poly(propylene glycol) in the presence of K₂CO₃ in hexamethylphosphoramide and N‐methylpyrrolidone, imidized at 200°C in nitrogen and the product was subsequently decomposed in air at 300°C to eliminate the labile PPG oligomer to produce PMDA/ODA polyimide nanofoam. Nuclear magnetic resonance spectroscopy (¹H‐NMR) and Fourier transform infrared spectroscopy (FT‐IR) techniques were used to characterize the formation of polyimide precursor and extent of grafting of PPG with polyimide. The results of thermogravimetric analysis (TGA) showed three step decomposition of nanofoam with the removal of PPG at 350°C and decomposition of polyimide at around 600°C. The polyimide nanofoams were also characterized by small angle X‐ray scattering (SAXS), field‐emission scanning electron microscopy (FE‐SEM) and transmission electron microscopy (TEM). The morphology showed nanophase‐separated structures with uniformly distributed and non‐interconnected pores of 20–40 nm in size. Dynamic mechanical analysis (DMA) indicated higher storage modulus for the foamed structure compared to the pure PI with reduction in loss tangent for the former system. Copyright © 2004 John Wiley & Sons, Ltd.     

Modulation masking produced by beating modulators.

This study examined whether "modulation masking" could be produced by temporal similarity of the probe and masker envelopes, even when the masker envelope did not contain a spectral component close to the probe frequency. Both masker and probe amplitude modulation were applied to a single 4-kHz sinusoidal or narrow-band noise carrier with a level of 70 dB SPL. The threshold for detecting 5-Hz probe modulation was affected by the presence of a pair of masker modulators beating at a 5-Hz rate (40 and 45 Hz, 50 and 55 Hz, or 60 and 65 Hz). The threshold was dependent on the phase of the probe modulation relative to the beat cycle of the masker modulators; the threshold elevation was greatest (12-15 dB for the sinusoidal carrier and 9-11 dB for the noise carrier, expressed as 20 log m) when the peak amplitude of the probe modulation coincided with a peak in the beat cycle. The maximum threshold elevation of the 5-Hz probe produced by the beating masker modulators was 7-12 dB greater than that produced by the individual components of the masker modulators. The threshold elevation produced by the beating masker modulators was 2-10 dB greater for 5-Hz probe modulation than for 3- or 7-Hz probe modulation. These results cannot be explained in terms of the spectra of the envelopes of the stimuli, as the beating masker modulators did not produce a 5-Hz component in the spectra of the envelopes. The threshold for detecting 5-Hz probe modulation in the presence of 5-Hz masker modulation varied with the relative phase of the probe and masker modulation. The pattern of results was similar to that found with the beating two-component modulators, except that thresholds were highest when the masker and probe were 180 degrees out of phase. The results are consistent with the idea that nonlinearities within the auditory system introduce distortion in the internal representation of the envelopes of the stimuli. In the case of two-component beating modulators, a weak component is introduced at the beat rate, and it has an amplitude minimum when the beat cycle is at its maximum. The results could be fitted well using two models, one based on the concept of a sliding temporal integrator and one based on the concept of a modulation filter bank.     

Identification of the Large-Conductance Ca2+-Regulated Potassium Channel in Mitochondria of Human Bronchial Epithelial Cells

Mitochondria play a key role in energy metabolism within the cell. Potassium channels such as ATP-sensitive, voltage-gated or large-conductance Ca²⁺-regulated channels have been described in the inner mitochondrial membrane. Several hypotheses have been proposed to describe the important roles of mitochondrial potassium channels in cell survival and death pathways. In the current study, we identified two populations of mitochondrial large-conductance Ca²⁺-regulated potassium (mitoBK_Ca) channels in human bronchial epithelial (HBE) cells. The biophysical properties of the channels were characterized using the patch-clamp technique. We observed the activity of the channel with a mean conductance close to 285 pS in symmetric 150/150 mM KCl solution. Channel activity was increased upon application of the potassium channel opener NS11021 in the micromolar concentration range. The channel activity was completely inhibited by 1 µM paxilline and 300 nM iberiotoxin, selective inhibitors of the BK_Ca channels. Based on calcium and iberiotoxin modulation, we suggest that the C-terminus of the protein is localized to the mitochondrial matrix. Additionally, using RT-PCR, we confirmed the presence of α pore-forming (Slo1) and auxiliary β3-β4 subunits of BK_Ca channel in HBE cells. Western blot analysis of cellular fractions confirmed the mitochondrial localization of α pore-forming and predominately β3 subunits. Additionally, the regulation of oxygen consumption and membrane potential of human bronchial epithelial mitochondria in the presence of the potassium channel opener NS11021 and inhibitor paxilline were also studied. In summary, for the first time, the electrophysiological and functional properties of the mitoBK_Ca channel in a bronchial epithelial cell line were described.     

Protein-Protein Interactions: Insight from Molecular Dynamics Simulations and Nanoparticle Tracking Analysis

Protein-protein interaction plays an essential role in almost all cellular processes and biological functions. Coupling molecular dynamics (MD) simulations and nanoparticle tracking analysis (NTA) assay offered a simple, rapid, and direct approach in monitoring the protein-protein binding process and predicting the binding affinity. Our case study of designed ankyrin repeats proteins (DARPins)—AnkGAG1D4 and the single point mutated AnkGAG1D4-Y56A for HIV-1 capsid protein (CA) were investigated. As reported, AnkGAG1D4 bound with CA for inhibitory activity; however, it lost its inhibitory strength when tyrosine at residue 56 AnkGAG1D4, the most key residue was replaced by alanine (AnkGAG1D4-Y56A). Through NTA, the binding of DARPins and CA was measured by monitoring the increment of the hydrodynamic radius of the AnkGAG1D4-gold conjugated nanoparticles (AnkGAG1D4-GNP) and AnkGAG1D4-Y56A-GNP upon interaction with CA in buffer solution. The size of the AnkGAG1D4-GNP increased when it interacted with CA but not AnkGAG1D4-Y56A-GNP. In addition, a much higher binding free energy (∆GB) of AnkGAG1D4-Y56A (−31 kcal/mol) obtained from MD further suggested affinity for CA completely reduced compared to AnkGAG1D4 (−60 kcal/mol). The possible mechanism of the protein-protein binding was explored in detail by decomposing the binding free energy for crucial residues identification and hydrogen bond analysis.     

Investigation of polyimides containing naphthalene units. III. Influence of monomers structure on polymers properties

A series of polymers from dinaphthalene dianhydrides and aromatic diamines has been synthesized using one-step high-temperature polycondensation. The influence of the monomers structure on the polymers properties has been investigated. © 1997 John Wiley & Sons, Inc.     

Magnetoliposomes as Potential Carriers of Doxorubicin to Tumours

Studies on magnetoliposomes (MLUV) as potential carriers for magnetic‐field‐dependent drug delivery are presented. The systems were formed with hydrophobic superparamagnetic iron oxide nanoparticles (SPIONs) confined within the bilayer of the liposomes. The nanomechanical properties of bilayer lipid membranes were evaluated and related to the amount of incorporated SPIONs. It was found that the presence of SPIONs in the lipid membrane leads to overall stiffening and increases morphological inhomogeneity, facilitating rupture of the MLUV membrane in a low‐frequency alternating magnetic field (AMF). To verify the findings, doxorubicin release from MLUVs in the presence and absence of an AMF was measured. Under experimental conditions, drug release proceeds through MLUV rupture induced by mechanical vibration of SPIONs rather than through localized heating in the vicinity of the SPIONs.