Improved analysis of ladderane lipids in biomass and sediments using high-performance liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry

Ladderane lipids, containing three or five linearly concatenated cyclobutane moieties, are considered to be unique biomarkers for the process of anaerobic ammonium oxidation, an important link in the oceanic nitrogen cycle. Due to the thermal lability of the strained cyclobutane moieties, the ladderane lipids are difficult to analyze by gas chromatography. A method combining high-performance liquid chromatography coupled to positive ion atmospheric pressure chemical ionization tandem mass spectrometry (HPLC/APCI-MS/MS) was developed for the analysis of the most abundant ladderane lipids, occurring as fatty acids and ether-bound to glycerol. Detection was achieved by selective reaction monitoring of four specific fragmentations per ladderane lipid. Detection limits of 30-35 pg injected on-column and a linear response (r(2) > 0.99) over nearly 3 orders of magnitude were achieved for all compounds. Using this method, these unique ladderane lipids were for the first time identified in a surface sediment from the Gullmarsfjorden, in concentrations ranging from 1.1-5.5 ng/g for the ladderane fatty acids and of 0.7 ng/g for the monoether. It is foreseen that this method will allow the investigation of the occurrence of anaerobic ammonium oxidation in natural settings in much greater detail than before.     

Potentiostat for use in electro-chemical experiments

Summary In many electro-chemical experiments it is desirable that one of the current-carrying electrodes of an electrolytic bath has a constant potential. This may be realized by manual control of the bath current. Owing to the sudden and unpredictable potential fluctuations of the electrode concerned, this is very difficult and even impossible in some special cases, e.g. when passivating an Fe-electrode in aqueous H₂SO₄. In addition there may be a considerable time-lag between the potential and the current fluctuations. The different types of potentiostats described so far seem to have too large a time-lag or/and too low a sensitivity for this purpose. The authors describe a potentiostat with proportional and integrating properties resulting in a stabilization of the potential within 1/2 mV in favourable sections of the potential current characteristic. Even with an electrode with a very steep potential current characteristic (e.g. the above mentioned Fe-electrode) the ripple never exceeds a few mV. The bath current, the maximum value of which is 30 mA, is the differential current of two electronic tubes. This type of circuit offers various advantages.     

Über die Einordnung der Affingeometrie in die Theorie der höheren Übertragungen

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Über die Einordnung der Affingeometrie in die Theorie der höheren Übertragungen

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Über die konforme Abbildungn-dimensionaler Mannigfaltigkeiten mit quadratischer Maßbestimmung auf eine Mannigfaltigkeit mit euklidischer Maßbestimmung

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Zur Geometrie der kontinuierlichen Transformationsgruppen

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Beiträge zur allgemeinen (gekrümmten) konformen Differentialgeometrie

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Zur Klassifizierung der assoziativen Zahlensysteme

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