NMR bioreactor development for live in-situ microbial functional analysis

A live, in-situ metabolomics capability was developed for prokaryotic cultures under controlled growth conditions. Toward this goal, a radiofrequency-transparent bioreactor was developed and integrated with a commercial wide-bore nuclear magnetic resonance (NMR) imaging spectrometer and a commercial bioreactor controller. Water suppressed 1H NMR spectroscopy was used to monitor glucose and fructose utilization and byproduct excretion by Eubacterium aggregans (an anaerobic bacterial species relevant for biofuel production) under controlled batch and continuous culture conditions. The resulting metabolite profiles (short chain organic acids and ethanol) and trends are consistent with existing knowledge of its metabolism. However, our study also showed that E. aggregans produces lactate end product in significant concentrations-a result not previously reported. The advantages of live in-situ microbial metabolomics analysis and its complementariness with functional genomics/systems biology methods are discussed.     

E1 calculations in thesdf-interacting boson model

A simple IBA-sdf form for theE1 transition operator containing an one-body term and a two-body term is tested in a non-analytical case. TheE 1 transition probabilities in the rare-earth region are reproduced rather well.     

Ordering and diffuse phase transitions in Pb(Sc0.5Ta0.5)O3 ceramics

The ordering of the Sc³⁺ and Ta⁵⁺ ions in Pb (Sc_0.5Ta_0.5) O₃ can be varied by proper heat treatments (1000–1500°C). The ferroelectric → paraelectric transition is strongly influenced by this ordering. With decreasing order parameter Ω the phase transition becomes more diffuse and a number of properties show strong changes.     

Mixed-symmetry states in the neutron-proton interacting boson model

States of mixed proton-neutron symmetry are investigated in different dynamical symmetries of the interacting boson model. We discuss in each of the limits the energy spectrum, the wave functions and the B(M1; 0₁⁺ → 1 _1⁺) values. We also study three classes of transitional nuclei namely the Pd nuclei [U(5) → O(6)], the Sm nuclei [U(5) → SU(3)] and the Pt nuclei [O(6) → SU(3)] with respect to the energy of the lowest non-symmetric J^π = 1⁺, 3⁺ levels as well as the M1 and M3 strengths for exciting these levels from the ground state. For ⁹⁸Pd we compare this calculation with a shell-model calculation. Finally, we adress the problem of the mixing of the non-symmetric J^π = 1⁺ state with nearby hexadecapole (g-boson) configurations.     

The even massN=83 isotones in the generalized seniority scheme

The results of a calculation in the generalized seniority scheme for the odd-oddN=83 isotones is presented. Results are compared with data and with a calculation in the framework of the IBA model. From the latter estimates for parameters in the bosonfermion interaction have been obtained.     

Polymerization of propadiene. IV. Kinetics and mechanism of polymerization under the influence of rhodium(I) complexes

The kinetics and mechanisms of propadiene polymerization under the influence of [Rh(CO)₂Cl]₂, Rh(CO)₂P(C₆H₅)₃Cl, Rh(CO)₃Cl are reported. The reaction rates are first-order in Rh(CO)₂P(C₆H₅)₃Cl and Rh(CO)₃Cl and half-order in [Rh(CO)₂Cl]₂. They are second-order in the substrate for Rh(CO)₃Cl and [Rh(CO)₂Cl]₂ and first-order for Rh(CO)₂P(C₆H₅)₃Cl. The data are interpreted in terms of a common intermediate mechanism. The formation of this common intermediate is the rate-determining step. A solvent effect is also discussed.     

Cooperative Effects in π-Ligand Bridged Dinuclear Complexes. XII [1]. Heterodinuclear Electron Poor μ-Cyclooctatetraene Complexes with CrFe-and CrCo-Combinations

The synfacial heterodinuclear μ-Cot complexes (Cot = cyclooctatetraene) [(CpCr) (CpM)]μ-Cot (Cp = cyclopentadienyl; M ? Fe, 3 ; M ? Co, 4 ) are formed in a thermal reaction of the mononuclear mixed sandwich compound CpCr(n⁶-Cot) and CpML_n [M ? Fe, L_n = benzene (Bz); M ? Co, L_n = (C₂H₄)₂]. 3 possesses two unpaired electrons whereas 4 has only one unpaired electron and is ESR active. From the molecular structure of 3 and from the ESR data of 4 it can be deduced that the unpaired electrons are localized at the Cr centers predominantly forcing a close electronical relation between the heterodinuclear compounds 3 and 4 and the mononuclear sandwich complexes chromocene and CpCrBz, respectively.