排序方式: 共有26条查询结果,搜索用时 15 毫秒
11.
应用新发展的单一轨迹积分方法求解库仑加线性位的基态量子波函数,得到基态能量和波函数的一般解析表达式,并讨论了解的收敛性.应用此方法讨论了重夸克偶素系统. 相似文献
12.
Sultana N Schenkman JB Rusling JF 《Journal of the American Chemical Society》2005,127(39):13460-13461
This communication demonstrates direct electron delivery from electrodes to cyt P450 reductases in stable films ( approximately 100 nm thick) of genetically enriched CYP1A2 and CYP3A4 microsomes made by layer-by-layer assembly with polyions. Reversible voltammetry of films containing genetically enriched cyt P450 monooxygenase microsomes was shown to involve cyt P450 reductase by comparison with the pure rabbit reductase and by lack of characteristic reactions of iron heme enzymes, such as reaction of the FeII form with CO and catalytic electrochemical reduction of oxygen and hydrogen peroxide. The microsome films were activated electrochemically to catalyze styrene epoxidation, consistent with the pathway utilized in the human liver, although further work is required to establish this definitively. 相似文献
13.
This study explored the ability of blind and sighted listeners to detect reflections, “echoes”, of burst trains or continuous noise. Echo detection was compared by presenting 5 ms bursts, rates from 1 to 64 bursts, with a continuous white noise, all during 500 ms. Sounds were recorded in an ordinary room through an artificial binaural head, the loudspeaker 1 m behind it. The reflecting object was an aluminum disk, diameter 0.5 m, placed at 1 m. The sounds were presented to 12 blind and 26 sighted participants in a laboratory using a 2-Alternative-Forced-Choice methodology. The task was to detect which of two sounds contained an echo. In Experiment 2, 1.5 m distance sounds were presented to the blind only. At 1 m, detection for the blind increased up to 64 bursts/500 ms, but for the sighted up to 32 bursts. At 1.5 m, the peak performance for the blind was at 32 bursts. At the 1 m, but not at the 1.5 m distance, the blind performed best with continuous white noise. The overlap in time of signal and echo at 1 m for 64 bursts was 60%, but at 1.5 m 82%. Avoiding an overlap between emitted bursts and returning echoes seems important for echolocation, indicating that an acoustic gaze, analogous to in echolocating animals, may also exist in humans. 相似文献
14.
We demonstrate for the first time a biosensor featuring a sequential two-enzyme pathway suitable to screen potentially toxic reactive metabolites generated during metabolism. 相似文献
15.
Rusling JF Hvastkovs EG Hull DO Schenkman JB 《Chemical communications (Cambridge, England)》2008,(2):141-154
This feature article summarizes recent applications of ultrathin films of enzymes and DNA assembled layer-by-layer (LbL). Using examples mainly from our own research, we focus on systems developed for biocatalysis and biosensors for toxicity screening. Enzyme-poly(L-lysine) (PLL) films, especially when stabilized by crosslinking, can be used for biocatalysis at unprecedented high temperatures or in acidic or basic solutions on electrodes or sub-micron sized beads. Such films have bright prospects for chiral synthesis and biofuel cells. Excellent bioactivity and retention of enzyme structure in these films facilitates their use in detailed kinetic studies. Biosensors and arrays employing DNA-enzyme films show great promise in predicting genotoxicity of new drug and chemical product candidates. These devices combine metabolic biocatalysis, reactive metabolite-DNA reactions, and DNA damage detection. Catalytic voltammetry or electrochemiluminescence (ECL) can be used for high throughput arrays utilizing multiple LbL "spots" of DNA, enzyme and metallopolymer. DNA-enzyme films can also be used to produce nucleobase adduct toxicity biomarkers for detection by LC-MS. These approaches provide valuable high throughput tools for drug and chemical product development and toxicity prediction. 相似文献
16.
17.
Zhou L Yang J Estavillo C Stuart JD Schenkman JB Rusling JF 《Journal of the American Chemical Society》2003,125(5):1431-1436
Rapid detection of DNA damage could serve as a basis for in vitro genotoxicity screening for new organic compounds. Ultrathin films (20-40 nm) containing myoglobin or cytochrome P450(cam) and DNA grown layer-by-layer on electrodes were activated by hydrogen peroxide, and the enzyme in the film generated metabolite styrene oxide from styrene. This styrene oxide reacted with double stranded (ds)-DNA in the same film, mimicking metabolism and DNA damage in human liver. DNA damage was detected by square wave voltammetry (SWV) by using catalytic oxidation with Ru(bpy)(3)(2+) (bpy = 2,2'-bipyridine) and by monitoring the binding of Co(bpy)(3)(3+). Damaged DNA reacts more rapidly than intact ds-DNA with Ru(bpy)(3)(3+), giving SWV peaks at approximately 1 V versus SCE that grow larger with reaction time. Co(bpy)(3)(3+) binds more strongly to intact ds-DNA, and its SWV peaks at 0.04 V decreased as DNA was damaged. Little change in SWV signals was found for incubations of DNA/enzyme films with unreactive organic controls or hydrogen peroxide. Capillary electrophoresis and HPLC-MS suggested the formation of styrene oxide adducts of DNA bases under similar reaction conditions in thin films and in solution. The catalytic SWV method was more sensitive than the Co(bpy)(3)(3+) binding assay, providing multiple measurements over a 5 min reaction time. 相似文献
18.
Dhanuka P. Wasalathanthri Dandan Li Donghui Song Zhifang Zheng Dharamainder Choudhary Ingela Jansson Xiuling Lu John B. Schenkman James F. Rusling 《Chemical science》2015,6(4):2457-2468
Human toxic responses are very often related to metabolism. Liver metabolism is traditionally studied, but other organs also convert chemicals and drugs to reactive metabolites leading to toxicity. When DNA damage is found, the effects are termed genotoxic. Here we describe a comprehensive new approach to evaluate chemical genotoxicity pathways from metabolites formed in situ by a broad spectrum of liver, lung, kidney and intestinal enzymes. DNA damage rates are measured with a microfluidic array featuring a 64-nanowell chip to facilitate fabrication of films of DNA, electrochemiluminescent (ECL) detection polymer [Ru(bpy)2(PVP)10]2+ {(PVP = poly(4-vinylpyridine))} and metabolic enzymes. First, multiple enzyme reactions are run on test compounds using the array, then ECL light related to the resulting DNA damage is measured. A companion method next facilitates reaction of target compounds with DNA/enzyme-coated magnetic beads in 96 well plates, after which DNA is hydrolyzed and nucleobase-metabolite adducts are detected by LC-MS/MS. The same organ enzymes are used as in the arrays. Outcomes revealed nucleobase adducts from DNA damage, enzymes responsible for reactive metabolites (e.g. cyt P450s), influence of bioconjugation, relative dynamics of enzymes suites from different organs, and pathways of possible genotoxic chemistry. Correlations between DNA damage rates from the cell-free array and organ-specific cell-based DNA damage were found. Results illustrate the power of the combined DNA/enzyme microarray/LC-MS/MS approach to efficiently explore a broad spectrum of organ-specific metabolic genotoxic pathways for drugs and environmental chemicals. 相似文献
19.
Krishnan S Wasalathanthri D Zhao L Schenkman JB Rusling JF 《Journal of the American Chemical Society》2011,133(5):1459-1465
Cytochrome (cyt) P450s comprise the enzyme superfamily responsible for human oxidative metabolism of a majority of drugs and xenobiotics. Electronic delivery of electrons to cyt P450s could be used to drive the natural catalytic cycle for fundamental investigations, stereo- and regioselective synthesis, and biosensors. We describe herein 30 nm nanometer-thick films on electrodes featuring excess human cyt P450s and cyt P450 reductase (CPR) microsomes that efficiently mimic the natural catalytic pathway for the first time. Redox potentials, electron-transfer rates, CO-binding, and substrate conversion rates confirmed that electrons are delivered from the electrode to CPR, which transfers them to cyt P450. The film system enabled electrochemical probing of the interaction between cyt P450 and CPR for the first time. Agreement of film voltammetry data with theoretical simulations supports a pathway featuring a key equilibrium redox reaction in the natural catalytic pathway between reduced CPR and cyt P450 occurring within a CPR-cyt P450 complex uniquely poised for substrate conversion. 相似文献
20.
We have deposited relatively thick (∼60 nm) Ge layers on Br-passivated Si(111) substrates by thermal evaporation under high
vacuum conditions at room temperature. Ge has grown in a layer-plus-island mode although it is different from the Stranski-Krastanov
growth mode observed in epitaxial growth. Both the islands and the layer are nanocrystalline. This appears to be a consequence
of reduction of surface free energy of the Si(111) substrate by Br-passivation. The size distribution of the Ge nanoislands
has been determined. The Br-Si(111) substrates were prepared by a liquid treatment, which may not produce exactly reproducible
surfaces. Nevertheless, some basic features of the nanostructural island growth are reasonably reproducible, while there are
variations in the details of the island size distribution. 相似文献