排序方式: 共有180条查询结果,搜索用时 31 毫秒
111.
112.
113.
114.
115.
Atsushi Shoji Erika Sugimoto Sayaka Orita Keiichiro Nozawa Akio Yanagida Yoichi Shibusawa Masao Sugawara 《Analytical and bioanalytical chemistry》2010,397(3):1377-1381
We describe a reusable liposome array based on the formation of cleavable disulfide cross-links between liposomes and the
surface of a glass slip. The N-succinimidyl 3-(2-pyridyldithio)-propionate (SPDP)-modified liposomes encapsulating a pH-sensitive fluorescence dye were
immobilized on a 3-mercaptopropyltrimethoxysilane (MTS)-modified glass slip through the formation of disulfide bonds. The
regeneration of a used slip was performed by the lysis of immobilized liposomes with Triton X-100 and the cleavage of disulfide
bonds by reduction with TCEP, followed by immobilization of SPDP-modified liposomes. The regeneration steps did not affect
the fluorescence intensity of re-immobilized liposomes. The liposome array was applied to simultaneous quantification of growth
hormone related peptides, i.e., GHRF and somatostatin, in a mixture. After optimizing the assay condition, the method allowed
quantification of GHRF and somatostatin in concentration ranges from 0.5 × 10−9 to 0.5 × 10−7 g/mL with detection limits of 2 × 10−10 and 3 × 10−10 g/mL, respectively. 相似文献
116.
Yushi Futamura Makoto Kawatani Sayaka Kazami Kenichi Tanaka Makoto Muroi Takeshi Shimizu Koji Tomita Nobumoto Watanabe Hiroyuki Osada 《Chemistry & biology》2012,19(12):1620-1630
Highlights? Development of an encyclopedia of cell morphology, Morphobase ? Small molecules classified by mode of action with Morphobase ? Morphobase system rapidly predicts molecular targets of compounds ? NPD6689 targets tubulin and is a lead for a class of antitubulin drugs 相似文献
117.
Kinoshita H Osamura T Mizuno K Kinoshita S Iwamura T Watanabe S Kataoka T Muraoka O Tanabe G 《Chemistry (Weinheim an der Bergstrasse, Germany)》2006,12(14):3896-3904
Reactions between chiral 3-cinnamoyl-4-methyl-5-phenyl-1,3-oxazolidine-2-thiones and aromatic aldehydes in the presence of BF3Et2O diastereoselectively produced tricyclic compounds incorporating a bridgehead carbon bound to four heteroatoms in high yields. Four stereocenters were induced during the reaction. The tricyclic products were transformed into propane-1,3-diols bearing three consecutive stereocenters by acid hydrolysis, S-methylation, and reductive removal of the chiral auxiliary. 相似文献
118.
119.
Shinji Nishikawa Sayaka Kami Nurul Ashikin Badrul Haswan Takahiro Uno Takahito Itoh Masataka Kubo 《Journal of polymer science. Part A, Polymer chemistry》2015,53(5):622-628
Polyfluorenes with pendant allyl groups were prepared by terpolymerization of 9,9‐dihexylfluorene‐2,7‐bis(trimethyleneborate), 2,7‐dibromo‐9,9‐bis(2‐allyloxyethyl)fluorene, and 4,7‐dibromo‐2,1,3‐benzothiazole, or 4,7‐dibromo‐2,1,3‐naphthoselenadiazole using Suzuki coupling reaction. The subsequent hydrosilylation reaction of these precursor polymers with ethoxydimethylsilane quantitatively converted the allyl groups to ethoxysilyl groups. Hybridization of the emitting polyfluorenes with silicone was successfully achieved by the solvent‐free sol–gel reaction of tetraethoxysilane and silanol‐terminated polydimethylsiloxane in the presence of the ethoxysilyl‐functionalized polyfluorenes. Fluorescence spectra of these luminescent silicones revealed that emitting polyfluorenes were dispersed homogeneously in the matrix of silicone without aggregation. © 2014 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2015 , 53, 622–628 相似文献
120.