Hypbrvalent Iodine Oxidation of 3-(2-Furyl)-1-(2-hydroxyaryl)prop-2-enones : Synthesis of Novel 2-(2-Furyl)-cis-3-hydroxvchromanones and Derived 3-Hydroxychromones

The synthesis of novel 2-(2-furyl)-cis-3-hydroxychro-manones(3a-c) has been achieved from 3-(2-furyl)-1-(2-hydroxy-aryl)-2-enones (1a-c) via acid catalyzed hydrolysis of dimethylacetals (2a-c), formed by hypervalent iodine oxidation using iodobenzene diacetate in methanolic potassium hydroxide. Action of dil. alkali on 3a and 3b provides a new synthesis of 2-(2-furyl)-3-hydroxychromones (6a, 6b).     

Simple and Convenient One‐Pot Synthesis of Benzimidazoles and Benzoxazoles using N,N‐Dimethylchlorosulfitemethaniminium Chloride as Condensing Agent

N,N‐Dimethylchlorosulfitemethaniminium chloride (SOCl₂‐DMF) has been found to be an efficient reagent for the one‐pot synthesis of benzimidazoles and benzoxazoles in excellent yield by condensation of carboxylic acids with o‐phenylenediamine/2‐amino‐phenol.     

Voice Handicap Index Results for Older Patients with Adductor Spasmodic Dysphonia

Adductor spasmodic dysphonia (ADSD) adversely affects a speaker's ability to effectively communicate. For many individuals suffering with ADSD, botulinum toxin (Botox) is the chosen treatment to remediate the symptoms. Although Botox's effects on symptom remediation have been examined before, patient perception of improvement post-Botox has been examined less frequently. Further, no studies have addressed the symptomatic changes in older adults that occur after Botox treatment. The Voice Handicap Index (VHI) was used as the instrument to assess older patient's (>65 years) perception of how ADSD impacts certain areas of their life pre- and post-Botox injection. The outcome of the VHI was related to a clinical judgment of voice severity. Participants also completed the Social Readjustment Rating Scale (SRRS) to examine overall stress level, and the outcome of the SRRS was correlated to postinjection VHI scores. Results indicated no significant correlation between VHI scores and voice severity or SRRS ratings. The current study suggests further study of voice outcomes with older adults with ADSD is needed.     

Self-assembly of cetylpyridinium chloride in water-DMF binary mixtures: a spectroscopic and physicochemical approach

The aggregation behavior of cetylpyridinium chloride (CPyCl) in N,N-dimethylformamide (DMF)-water mixed solvents was investigated using electrical conductivity and spectroscopic techniques. Micellar and thermodynamic parameters (DeltaG(m)(0), DeltaH(m)(0), DeltaS(m)(0) and Delta(m)C(p)(0)) were obtained from the temperature dependence of critical micelle concentrations in various aqueous mixtures of DMF. The differences in the Gibbs energies of micellization of CPyCl between water and binary solvents were determined to evaluate the influence of the cosolvent. The effect of cosolvent on the Krafft temperature (K(T)) and on the aggregation number was also analyzed. Micellar micropolarity was examined spectrophotometrically using two different probes, methyl orange (MO) and methylene blue (MB), and was found to increase with DMF addition, accompanied by an enhanced solvation. The mechanism of docking of surfactant and the probe molecules in the system were obtained by using Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) spectroscopy.     

Purification and characterization of a native zinc-binding high molecular weight multiprotein complex from human seminal plasma

The seminal plasma comprises secretions from various accessory sex glands. During fertilization spermatozoa undergo complex sequences of precisely timed events that are regulated by the activation of different intracellular signaling pathways. The precision and efficacy of these pathways are often influenced by the assembly and interactions of multiprotein complexes, thereby directing the flow of regulatory information. Our knowledge about these protein complexes present in human seminal plasma (HuSP) is limited. Here we report the identification and characterization of a native high molecular weight zinc-binding multiprotein complex from HuSP by utilizing 2-DE followed by MS. Twenty-six proteins representing isoforms and/or fragments of 11 different proteins were found to be assembled in this complex. Prostate-specific antigen, zinc α2-glycoprotein, prostatic acid phosphatase, and prolactin inducible protein were the major proteins of this complex. Dynamic light scattering experiments revealed changes in aggregation pattern accompanied with deviation from physiological pH and in presence of SDS. However, no significant changes were observed in the presence of physiological ligands such as zinc and fructose. The present study will be useful and contribute to guide the future studies performed for elucidation of biological significance of this native complex in HuSP.     

Kinetic study of oxidation of galactose by N-bromo phthalimide in the presence of cationic micelle in acidic medium

The kinetics of micellar catalyzed oxidation of galactose by N-bromophthalimide was studied in the presence of acidic medium at 308?K. The oxidation reaction exhibits first-order kinetics with respect to oxidant (N-bromophthalimide), fractional order with respect to substrate (galactose) and positive fractional order with respect to HClO₄ on the rate of reaction. The rate of the reaction increased with decreasing the dielectric constant of the medium. With a progressive increase in the concentration of CTAB, the rate of reaction increased and after reaching peak k _obs, decreased at higher concentrations of CTAB. There catalytic roles are best explained by Berezin??s model. The influence of salts on the reaction rate was also studied. The various activation parameters have been calculated. The rate constant and binding constant with the surfactant have also been evaluated. A suitable mechanism consistent with the experimental findings has been proposed.     

Solutions of the SchrödingerEquation forPT-Symmetric Coupled Quintic Potentialsin Two Dimensions

This paper deals with the solutions of time independent Schrödinger wave equation for a two-dimensional PT-symmetric coupled quintic potential in its most general form. Employing wavefunction ansatz method, general analytic expressions for eigenvalues and eigenfunctions for first four states are obtained. Solutions of a particular case are also presented.     

Modulation in Techno-Functional,Textural Properties,In Vitro Starch Digestibility and Macromolecular–Structural Interactions of Pasta with Potato (Solanum tuberosum L.)

The replacement of semolina with potato flour (PF) and potato mash (PM) at different levels was assessed for its effects on pasta quality. The results showed that the addition of PF and PM increased the pasting viscosity of the blends; in addition, PF enhanced the functional properties, while PM reduced them. The minimum cooking time decreased with PF and PM, while the PF pasta exhibited a higher cooking loss (5.02 to 10.44%) than the PM pasta, which exhibited a lower cooking loss. The pasta with PF and PM showed an increase in the total phenolic and flavonoid content, with reduced in vitro digestibility as confirmed by Fourier transform infrared spectroscopy. The PF pasta exhibited lower lightness and higher yellowness than the PM pasta, and its firmness and toughness also modulated owing to the complex interaction between potato starches and the gluten protein matrix, as evident from scanning electron microscopy. Sensory data revealed that pasta containing 30% PF and 16% PM was highly acceptable.     

Expeditious novel routes to enantiopure 3-amino tetrahydrofuran hydrochloride

The synthesis of chemically and enantiomerically pure (S)-3-amino tetrahydrofuran hydrochloride starting from the natural amino acids, l-aspartic acid or l-methionine is described. The process involves no chromatography and can be easily carried out on a large scale. The enantiopurity of the final product was established by NMR and chiral HPLC methods.     

Sample complexity reduction aids efficient detection of low‐abundant proteins from human amniotic fluid

Working with biological fluids poses a challenge of visualizing proteins present in lower concentrations. This study describes a batch‐mode chromatographic method for the fractionation of human amniotic fluid (AF). This method is easy to use with minimal sample quantity, resin volume and sample processing time. For albumin depletion, two methods were evaluated. The results demonstrated that specific depletion of albumin, using affinity‐ligand‐based resin, is more efficient than the conventional dye‐based method. The albumin‐depleted human AF was fractionated by strong anion‐exchange resin in spin devices, for sample, complexity reduction and enrichment of low‐abundant proteins. Analysis of four eluate fractions generated after this step shows enrichment of few low‐abundant proteins. Two novel low‐abundant proteins, Rab GDP dissociation inhibitor β and peptide methionine sulfoxide reductase, were identified from human AF. α‐1‐B Glycoprotein was successfully identified by this strategy, whereas the published literature reports that it was not identified by strong anion‐exchange FPLC followed by SDS‐PAGE. Therefore, the current method has distinct advantages over the conventional column‐based chromatography. This study also reports altered expression of some proteins in Rh‐isoimmunized AF samples in comparison with normal AF.