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991.
Surface plasmon resonance (SPR) technique is used to follow, both in real time and in situ, the association between a physically adsorbed polymer of beta-cyclodextrin (pbetaCD) and different hydrophobically modified poly(N-isopropylacrylamide) (PNIPAM) copolymers containing either adamantyl or dodecyl groups. This association is due to the complex formation between the hydrophobic groups and the betaCD cavities. Therefore, the adsorbed amount of PNIPAM onto the pbetaCD layer depends on the substituent and on its substitution level. The association and dissociation rate constants are evaluated from the kinetics of PNIPAM adsorption. An estimation of the association constants leads to values higher than 10(4) M(-1), reflecting the strong interaction between these polymers. 相似文献
992.
Faull KF Johnson J Kim MJ To T Whitelegge JP Stevens RL Fluharty CB Fluharty AL 《Journal of mass spectrometry : JMS》2000,35(12):1416-1424
The specific sugar residues and their linkages in the oligosaccharides from pig kidney and human urine cerebroside sulfate activator proteins (saposin B), although previously hypothesized, have been unambiguously characterized. Exhaustive sequential exoglycosidase digestion of the trimethyl-p-aminophenyl derivatives, followed by either matrix-assisted laser desorption/ionization and/or mass spectrometry, was used to define the residues and their linkages. The oligosaccharides were enzymatically released from the proteins by treatment with peptidyl-N-glycosidase F and separated from the proteins by reversed-phase high-performance liquid chromatography (HPLC). Reducing termini were converted to the trimethyl-p-aminophenyl derivative and the samples were further purified by normal-phase HPLC. The derivatized carbohydrates were then treated sequentially with a series of exoglycosidases of defined specificity, and the products of each digestion were examined by mass spectrometry. The pentasaccharides from pig kidney and human urine protein were shown to be of the asparagine-linked complex type composed of mannose-alpha 1-6-mannose-beta 1-4-N-acetylglucosamine-N-acetylglucosamine(alpha 1-6-fucose). This highly degraded structure probably represents the final product of intra-lysosomal exoglycosidase digestion. Oligosaccharide sequencing by specific exoglycosidase degradation coupled with mass spectrometry is more rapid than conventional oligosaccharide sequencing. The procedures developed will be useful for sequencing other oligosaccharides including those from other members of the lipid-binding protein class to which cerebroside sulfate activator belongs. (c) 2000 John Wiley & Sons, Ltd. 相似文献
993.
994.
Chemiluminescence immunoassay for the detection and quantification of methyltestosterone residues in muscle tissue 总被引:1,自引:0,他引:1
Chemiluminescence as a detection method for immunoassay has successfully been applied to the measurement of methyltestosterone (MT) residues in muscle tissue. The sample is digested enzymatically, extracted with diethyl ether and purified on a Lipidex-5000 column. An optional clean-up utilized disposable C18 columns. As the luminescent label the N-(4-aminobutyl)-N-ethylisoluminol conjugate of MT was used. The antiserum was raised in a rabbit against MT-3-carboxymethyloxime-bovine serum albumin. The detection limit of the assay was 14 +/- 7 pg (n = 13), with a limit of quantification in muscle tissue of 0.125 ppb. 相似文献
995.
The electrochemical oxidation of ethyl 3-oxo-3-phenyl-2-phenylhydrazonopropionate has been studied in the pH range 3.0–11.0 at a pyrolytic graphite electrode by linear and cyclic sweep voltammetry, coulometry and spectral studies. The results indicate that the 2 e−, 2 H+ oxidation of this compound gives phenol and ethyl 3-phenyl-2,3-dioxopropionate as the major products of electrooxidation. 相似文献
996.
G L Grunewald M A Pleiss C L Gatchell R Pazhenchevsky M F Rafferty 《Journal of chromatography. A》1984,292(2):319-331
The elution behaviour of a selection of sequence variant and modified insulins has been compared on a C18 reversed-phase column. Observed elution times were compared with those expected from the nature of the differences from bovine insulin. In some cases prediction rules established for peptides are adequate to explain the observed elution and in others detailed knowledge of the structure of the protein is of considerable importance in understanding elution behaviour. 相似文献
997.
M. Cotrait E. Dupart J. Prigent C. Garrigou-Lagrange 《Journal of Molecular Structure》1978,50(2):313-324
β-Alanyl aminomethylphosphonic acid (β-ala AMPh) has been studied in the solid state by X-ray diffraction and vibration spectrometry. It crystallizes (space group P1&;#x0304; with Z = 4) as a zwitterion with, in the asymétrie unit, two independent molecules the conformations of which are quite different. The hydrogen bonding network is very complicated. Because the infrared spectra are very complicated, only a partial correlation of the vibrations of ammonium, amide and phosphonic groups is proposed. 相似文献
998.
999.
A rapid, selective and reproducible high-performance liquid chromatographic (HPLC) method with ultraviolet detection was developed for the determination of the anti-cancer agent Taxotere in biological fluids. The method involves a solid-phase extraction step (C2 ethyl microcolumns) using a Varian Advanced Automated Sample Processor (AASP) followed by reversed-phase HPLC. The validated quantitation range of the method is 10-2500 ng/ml in plasma with coefficients of variation < or = 11%. The method is also suitable for the determination of Taxotere in urine samples under the same conditions. The method was applied in a phase I tolerance study of Taxotere in cancer patients, allowing the pharmacokinetic profile of Taxotere to be established. 相似文献
1000.
G. Merle J. P. Pascault Q. T. Pham C. Pillot R. Salle J. Gol I. Rashkov I. Panayotov D. Gurard A. Hrold 《Journal of polymer science. Part A, Polymer chemistry》1977,15(9):2067-2074
In the present work, we use the binary insertion compound LiC12 to polymerize styrene, methyl methacrylate, butadiene, isoprene, and to copolymerize isoprene and styrene in various hydrocarbon solvents (aromatics and aliphatic) and etheral solvents. We show that the styrene polymerization in aromatic solvents gives better yields than in the etheral solvents, the polymer being atactic. Methyl methacrylate does not polymerize in toluene but does so completely in DME. More generally, the yields of polymerization are better with KC37 than with LiC12 because of the different capacities of the monomer to get into the carbon layers. The polymerization of dienes with LiC12 shows that the microstructures of the polymer obtained in π-or n-donor solvents are similar to the ones obtained by homogenous polymerization with Li cation in such solvents. However, for isoprene in cyclohexane, the results are different. The isoprene styrene copolymers are statistical ones and the mean length of styrene blocks is less than 5. The monomer interaction with the insertion compound and the growing chain geometry between the carbon layers are the facts which control either the stereospecificity of the polymerization or the selectivity of the copolymerization. 相似文献