Investigating cellular metabolism of synthetic azidosugars with the Staudinger ligation

The structure of sialic acid on living cells can be modulated by metabolism of unnatural biosynthetic precursors. Here we investigate the conversion of a panel of azide-functionalized mannosamine and glucosamine derivatives into cell-surface sialosides. A key tool in this study is the Staudinger ligation, a highly selective reaction between modified triarylphosphines and azides that produces an amide-linked product. A preliminary study of the mechanism of this reaction, and refined conditions for its in vivo execution, are reported. The reaction provided a means to label the glycoconjugate-bound azidosugars with biochemical probes. Finally, we demonstrate that the cell-surface Staudinger ligation is compatible with hydrazone formation from metabolically introduced ketones. These two strategies provide a means to selectively modify cell-surface glycans with exogenous probes.     

Pharmacological interactions of essential oil constituents on the viability of micro-organisms

Complex interactions between numerous components of essential oils often contribute to the pharmacological effect and therapeutic outcome. To further elucidate these interactions, several essential oil constituents (EOCs) were combined in different ratios and their inhibitory effects on the growth of bacteria and yeast determined using the minimum inhibitory concentration (MIC) microplate assay. When combined and tested against Candida albicans, (+)-beta-pinene interacted antagonistically with (-)-menthone (sigmaFIC(T) = 9.80), but synergistically with 1,8-cineole (sigmaFIC(T) = 0.35). Against Escherichia coli, the combination of E- and Z-(+/-)-nerolidol and geranyl acetate displayed an additive interaction (sigmaFIC(T) = 1.04); while a variable interaction was observed between E- and Z-(+/-)-nerolidol and eugenol with antagonism and synergy being observed at different ratios of each EOC. The combination of either carvacrol or eugenol with an antimicrobial agent (ciprofloxacin or amphotericin B) resulted in synergistic interactions against all microorganisms tested. These favourable results further support the use of essential oil constituents as adjuvants in the development of a new generation of phytopharmaceuticals that can be used in combination with synthetic drugs against drug-resistant microorganisms.     

New strategies to study the chemical nature of wine oligomeric procyanidins

Tannins represent a key element in red wine flavors, so researchers have made a lot of effort to try to understand the role of their structure in wine taste in recent decades. We report some new routes to achieve a true structure–taste relationship for the major tannins found in wine, which are procyanidins in their monomeric or oligomeric state. All these routes use synthetic standards. New advances in their synthesis and their analyses using chromatographic methods, NMR spectroscopy, and mass spectrometry to obtain more precise information about their chemical structure, including their stereochemistry and their precise degree of polymerization and galloylation, are described.     

Lewis’ Reduction of Modality

I start by reconsidering two familiar arguments against modal realism. The argument from epistemology relates to the issue whether we can infer the existence of concrete objects by a priori means. The argument from pragmatics purports to refute the analogy between the indispensability of possible worlds and the indispensability of unobserved entities in physical science and of numbers in mathematics. Then I present two novel objections. One focusses on the obscurity of the notion of isolation required by modal realism. The other stresses the arbitrary nature of the rules governing the behaviour of Lewisean universes. All four objections attack the reductive analysis of modality that is supposed to be the chief merit of modal realism.     

X‐ray speckle visibility spectroscopy in the single‐photon limit

The technique of speckle visibility spectroscopy has been employed for the measurement of dynamics using coherent X‐ray scattering. It is shown that the X‐ray contrast within a single exposure can be related to the relaxation time of the intermediate scattering function, and this methodology is applied to the diffusion of 72 nm‐radius latex spheres in glycerol. Data were collected with exposure times as short as 2 ms by employing a resonant shutter. The weak scattering present for short exposures necessitated an analysis formalism based on the spatial correlation function of individual photon charge droplets on an area detector, rather than the usual methods employed for intensity correlations. It is demonstrated that this method gives good agreement between theory and experiment and thus holds promise for extending area‐detector‐based coherent scattering methods to the study of faster dynamics than previously obtainable.     

Conditions for the generalized numerical range to be real

Necessary and sufficient conditions are given for the C-numerical range of a matrix A to be a subset of the real axis. In particular, it is shown that both A and C must be translates of hermitian matrices.     

Mathematical Models for Studying the Value of Motivational Leadership in Teams

Mathematical models are presented for studying the value of leadership in a team where the members interact with each other. The models are based on a leader’s role of motivating each team member to perform closer to his/her maximum ability. These models include controllable parameters whose values reflect the amount of task interdependence among the workers as well as the motivational skill and variability in the skill of the leader. Confirming results—such as the fact that the skill level of the leader is a critical factor in the expected performance of the team—establish credibility in the models. Mathematical analysis and computer simulations are used to provide new managerial insights into the value of the leader—such as the fact that the skill of the leader can be more important than controlling the amount of interdependence among the team members and that having a choice of multiple leaders with no particular motivating skill is beneficial to the performance of small teams but not to large teams.Daniel Solow received a B.S. in Mathematics from Carnegie-Mellon, an M.S. in Operations Research from the University of California at Berkeley, and a Ph. D. in Operations Research from Stanford University. He has been a professor at Case Western Reserve University since 1978. His research interests include complex systems, discrete, linear, and nonlinear optimization. He has also developed systematic methods for teaching mathematical proofs, computer programming, and operations research.Sandy Kristin Piderit is an assistant professor of organizational behavior at the Weatherhead School of Management at Case Western Reserve University, and earned her Ph.D. from the University of Michigan. She studies the roles of relationships among coworkers on their performance and satisfaction with their work environments, and has published studies in the Academy of Management Review, the Journal of Management Studies, and Management Science.Apostolos Burnetas received a Diploma in Electrical Engineering from National Technical University in Athens, Greece, and an M.B.A. and Ph.D. in Operations Research from Rutgers University. He has been at the Department of Operations at Case Western Reserve University and is currently an Associate Professor at the Department of Mathematics at the University of Athens. His research interests include stochastic models and optimization, complex systems, and applications in queueing systems, supply chain and the interface of operations with finance.Chartchai Leenawong received a B.S. in Mathematics from Chulalongkorn University in Bangkok, an M.S. in Computer Science from the Asian Institute of Technology in Bangkok, and a Ph.D. in Operations Research from Case Western Reserve University. His research interests include mathematical modeling of complex systems as applied to business organizations. He has been a professor at King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand since 2002.     

Loss effects on adhesively-bonded multilayer ultrasonic transducers by self-heating

Multilayer ultrasonic transducers are widely being used for high power applications. In these applications, typical Langevin/Tonpilz structures without any adhesive bondings however have the disadvantage of limited bandwidth. Therefore adhesively-bonded structures are still a potential solution for this issue. In this paper, two-layer piezoelectric ceramic ultrasonic transducers with two different adhesive bondlines were investigated comparing to a single-layer transducer in terms of loss effects during operation with excitation signals sufficient to cause self-heating. The theoretical functions fitted to the measured time–temperature dependency data are compared with experimental results of different piezoelectric transducers. Theoretical analysis of loss characteristics at various surface displacements and the relationship with increasing temperature are reported. The effects of self-heating on the practical performance of multilayer ultrasonic transducers with adhesive bondlines are discussed.     

Thickness induced structural changes in polystyrene films

Changes to the structure of polystyrene melt films as measured through the spectrum of density fluctuations have been observed as a function of film thickness down to the polymer radius of gyration (Rg). Films thicker than 4Rg show bulklike density fluctuations. Thinner films exhibit a peak in S(q) near q=0 which grows with decreasing thickness. This peak is attributed to a decreased interpenetration of chains resulting in an enhanced compressibility. Measurements were made using small angle x-ray scattering in a standing wave geometry designed to enhance scattering from the interior of the film compared to interface scattering.