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21.
Electrochemical Detection of a Cancer Biomarker mir‐21 in Cell Lysates Using Graphene Modified Sensors
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Tugba Kilic Arzum Erdem Yasemin Erac M. Ozgur Seydibeyoglu Salih Okur Mehmet Ozsoz 《Electroanalysis》2015,27(2):317-326
In the present study, the voltammetric and impidimetric detection of microRNA‐21, mir‐21 from cell lysates was investigated for the first time by using graphene modified disposable pencil graphite electrodes (GME). The surface characterization of GME was performed via electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM). Upon passive adsorption of inosine substituted antimicroRNA‐21, antimir‐21 probe, InP, onto the surface of GME and then solid phase hybridization of InP with mir‐21, the target, the electrochemical detection was performed by using Differential Pulse Voltammetry (DPV) and EIS techniques. This developed biosensor, GME has presented a 2.77 times lower detection limit of 2.09 µg/mL (3.12 pmol) with respect to unmodified pencil graphite electrode (GE). Moreover it is capable of analyzing mir‐21 in the cell lysates of mir‐21 positive breast cancer cell line (MCF‐7) contrast to mir‐21 negative hepatoma cell line (HUH‐7). The proposed electrochemical yes‐no system does not require any purification and/or amplification step prior to fast detection of mir‐21 from real samples. 相似文献
22.
The aim of this work is to establish the existence of multi-peak solutions for the following class of quasilinear problems where \(\epsilon\) is a positive parameter, \(N\geq2\), \(V\), \(f\) are continuous functions satisfying some technical conditions and \(\phi\) is a \(C^{1}\)-function.
相似文献
$$ - \mbox{div} \bigl(\epsilon^{2}\phi\bigl(\epsilon|\nabla u|\bigr)\nabla u \bigr) + V(x)\phi\bigl(\vert u\vert\bigr)u = f(u)\quad\mbox{in } \mathbb{R}^{N}, $$
23.
In this study, new xanthine biosensors, XO/Au/PVF/Pt and XO/Pt/PVF/Pt, based on electroless deposition of gold(Au) and platinum(Pt) nanoparticles on polyvinylferrocene(PVF) coated Pt electrode for detection of xanthine were presented. The amperometric responses of the enzyme electrodes were measured at the constant potential, which was due to the electrooxidation of enzymatically produced H2O2. Compared with XO/PVF/Pt electrode, XO/Au/PVF/Pt and XO/Pt/PVF/Pt exhibited excellent electrocatalytic activity towards the oxidation of the analyte. Effect of Au and Pt nanoparticles was investigated by monitoring the response currents at the different deposition times and the different concentrations of KAuCl4 and PtBr2. Under the optimal conditions, the calibration curves of XO/Au/PVF/Pt and XO/Pt/PVF/Pt were obtained over the range of 2.5 × 10?3 to 0.56 mM and 2.0 × 10?3 to 0.66 mM, respectively. The detection limits were 7.5 × 10?4 mM for XO/Au/PVF/Pt and 6.0 × 10?4 mM for XO/Pt/PVF/Pt. The effects of interferents, the operational and the storage stabilities of the biosensors and the applicabilities of the proposed biosensors to the drug samples analysis were also evaluated. 相似文献
24.
Arndt M Salih KS Fromm A Goossen LJ Menges F Niedner-Schatteburg G 《Journal of the American Chemical Society》2011,133(19):7428-7449
The ruthenium-catalyzed hydroamidation of terminal alkynes has evolved to become a broadly applicable tool for the synthesis of enamides and enimides. Depending on the catalyst system employed, the reaction leads chemo-, regio-, and stereoselectively to a single diastereoisomer. Herein, we present a comprehensive mechanistic study of the ruthenium-catalyzed hydroamidation of terminal alkynes, which includes deuterium-labeling, in situ IR, in situ NMR, and in situ ESI-MS experiments complemented by computational studies. The results support the involvement of ruthenium-hydride and ruthenium-vinylidene species as the key intermediates. They are best explained by a reaction pathway that consists of an oxidative addition of the amide, followed by insertion of a π-coordinated alkyne into a ruthenium-hydride bond, rearrangement to a vinylidene species, nucleophilic attack of the amide, and finally reductive elimination of the product. 相似文献
25.
The differential cross sections for coherent scattering of 59.5 keV γ-rays by Mo and Sn were measured using a high-purity germanium detector. The results were compared with predictions of form factor theories and S-matrix calculations. 相似文献
26.
We discuss the parametrization of quantum groups in terms of independent operators. We find that this consideration leads to the parametrization ofSU q(2) in terms of aq-oscillator plus a commuting phase. The commuting phase is naturally identified with the subgroupU(1) and the remaining cosetSU q(2)/U(1)=CP q(1) consists of aq-oscillator. For unitary quantum groupsSU q (n), the analogous construction results in the quantum projective spaceSU q(n+1)/U q (n)=CP q (n) being identified with then-dimensionalq-oscillator. This yields a nonlinear action of the quantum groupSU q(n+1) on then-dimensionalq-oscillator. 相似文献
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28.
Ohne ZusammenfassungFür viele wertvolle Hinweise möchte ich an dieser Stelle meinem akademischen Lehrer, Herrn Prof. Dr. W. von Wahl, danken 相似文献
29.
Experiment of C-Band Wavelength Conversion in a Silicon Waveguide Pumped by Dispersed Femtosecond Laser Pulse
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We experimentally demonstrate the C-band wavelength conversion using four-wave mixing in a 17-mm-long silicon-on-insulator waveguide pumped by a dispersed mode-locked femtosecond laser pulse. The idler can be observed with an incident average pump power lower than 4 dBm, and about 35 nm of conversion bandwidth from 1530nm to 1565nm is measured by using a 1550-nm pump wavelength. The pulse-pumped efficiency is demonstrated to be higher, by more than 22 dB, than the cw-pumped efficiency. The conversion efficiency variations with respect to the pump and signal powers are also investigated. 相似文献
30.
In this paper, we investigate with a time fractional-order derivative in a three-species predator-prey model with the presence of prey social behavior. A new approximation for predator-prey interaction in the presence of prey social behavior has been considered. For the model analysis, the study has been divided into two principal parts. First of all, we study the local stability of the equilibria and the existence of Hopf bifurcation. Then, for the numerical analysis, the Caputo fractional derivative operator is utilized to approximate the numerical solution of the model. An excellent agreement is seen between the numerical results and the theoretical predictions. 相似文献