牛血清白蛋白与两种金属配合物的作用研究

The interaction of Methylthymol Blue(MTB)-Zinc(Ⅱ) compound and Alizarin Red S(ARS)-Aluminum(Ⅲ) compound with Bovine serum albumin (BSA) was investigated by UV-Vis spectrophotometric method in acidic buffer solution. MTB-Zn(Ⅱ)-BSA was a blue color compound, which possesses maximum absorption at 613 nm with 172 nm, 174 nm and 18 nm of red shift compared to the MTB, MTB-BSA and MTB-Zn(Ⅱ) complexes respectively. Dual wavelength substantial amount ratio method, balance dialysis substantial amount ratio method and unity wavelength substantial amount ratio method were compared. The following results were obtained: the apparent molar absorptivity of MTB-Zn(Ⅱ) with BSA was ε=2.20×10⁴ L·mol^-1·cm^-1. Conditional proportion were defined, n_MTB∶n_Zn(Ⅱ)∶n_BSA=2∶2∶1; condition combination constant, K=2.07×1010. Combination proportion were defined, n_ARS∶n_Al(Ⅲ)∶n_BSA=6∶4∶1. Condition equilibrium constant of reaction of ARS-Al(Ⅲ) with BSA was K=8.80×10⁸. The apparent molar absorptivity of ε=2.65×10⁴ L·mol^-1·cm^-1. It is suggested that combination between BSA and MTB-Zn(Ⅲ) is due to coordination force. That combination between BSA and ARS-Al(Ⅲ) is due to the coordination bond and electrostatic force.     

Synthesis and solution studies of the complexes of trivalent lanthanides with the tetraazamacrocycle TETA-(PO)(2)

A new potentially multidentate hexaprotic ligand H(6)[TETA-(PO)(2)] has been prepared by reaction of ethylenediamine-N,N'-diacetic acid (EDDA), paraformaldehyde, and phosphinic acid; its coordination properties with three lanthanide ions (La(3+), Gd(3+), and Lu(3+)) have been explored. The structures of the complexes were studied in aqueous solution by potentiometric pH titrations and by (31)P NMR spectroscopy. Four acidity constants were determined potentiometrically in the range 2.5 < pH < 14. The four measured pK(a) values can be divided into two groups, and within each group the initial deprotonation was found to have little effect on the second. Variable temperature (31)P and (31)P[(1)H] EXSY NMR spectra showed that, for [Lu(TETA-(PO)(2))](3-), the two phosphorus atoms exist in different chemical environments and undergo an exchange process which is very fast on the NMR time scale at room temperature. This result is consistent with one of the phosphinate residues coordinating the metal ion and exchanging with a free analogue. In the case of [La(TETA-(PO)(2))](3-), only one temperature invariant signal is observed in (31)P NMR spectra; it corresponds to both phosphinate residues remaining uncoordinated to La(3+). The stability of [Ln(TETA-(PO)(2))](3-) has an order of La(3+) > Gd(3+) > Lu(3+). The coordination of one phosphinate residue to Lu(3+) brings the metal ion closer to the plane of four nitrogens and farther from the four carboxylate arms, resulting in [Lu(TETA-(PO)(2))](3-) having a lower stability than the corresponding La(3+) and Gd(3+) complexes. A pM-pH distribution diagram showed that introducing two phosphinate groups into TETA renders [Gd(TETA-(PO)(2))](3-) more stable than [Gd(TETA)](-). The selectivity factor of the ligand for Gd(3+) vs Ca(2+), Zn(2+), and Cu(2+) has been calculated, and the hydration number for [Dy(TETA-(PO)(2))](3-) has been measured by (17)O NMR spectroscopy to be zero.     

不同分子量N-琥珀酰壳聚糖的制备及其与K562肿瘤细胞间亲和性的初步探讨

用化学降解法制备不同分子量的壳聚糖 ,以其为原料合成了系列N 琥珀酰壳聚糖 ,然后用异硫氰酸荧光素进行荧光标记 ,再与K5 6 2肿瘤细胞共孵育 ,通过流式细胞仪检测细胞的荧光强度来确定不同分子量N 琥珀酰壳聚糖与K5 6 2肿瘤细胞间亲和性的强弱 ,为靶向抗肿瘤药物载体的研究提供初步的参考 .结果表明N 琥珀酰壳聚糖和K5 6 2肿瘤细胞间有较强的亲和性 ,随着分子量的增加 ,其亲和性逐渐减弱 .     

Clean Aerobic Liquid Oxidation of Aldehydes with Solid Catalyst

Clean liquid oxidation of aldehydes can be accomplished using solid catalyst in the presence of molecular oxygen at room temperature, which is a valuable alternative to traditional counterparts.     

CertainC*-algebras with real rank zero and their corona and multiplier algebras: II

We first determine the homotopy classes of nontrivial projections in a purely infinite simpleC*-algebraA, in the associated multiplier algebraM(A) and the corona algebraM A/A in terms ofK _*(A). Then we describe the generalized Fredholm indices as the group of homotopy classes of non-trivial projections ofA; consequently, we determine theK _*-groups of all hereditaryC*-subalgebras of certain corona algebras. Secondly, we consider a group structure of *-isomorphism classes of hereditaryC*-subalgebras of purely infinite simpleC*-algebras. In addition, we prove that ifA is aC*-algebra of real rank zero, then each unitary ofA, in caseA it unital, each unitary ofM(A) and ofM(A)/A, in caseA is nonunital but -unital, can be factored into a product of a unitary homotopic to the identity and a unitary matrix whose entries are all partial isometries (with respect to a decomposition of the identity).Partially supported by a grant from the National Science Foundation.     

1,6-六亚甲基二异氰酸酯自聚产物的结构表征

用IR与NMR表征了用醋酸钾为催化剂时 1,6 六亚甲基二异氰酸酯 (HDI)自聚产物的结构 .结果表明 ,自聚主产物是三聚体异氰脲酸酯 ,主要含有三聚体异氰脲基、异氰酸根 ,同时含有由杂质带来的微量氨基甲酸酯、脲基甲酸酯基、取代脲基、缩二脲基 .一维核磁谱及二维化学位移相关谱分辨出 7种羰基 ,一种NCO基 ,确定了氮上 8种不同取代结构的分子链连接情况 .通过建立理论模型 ,定量地描述了自聚产物的结构 .     

Comparative study on the inclusion behaviour of cyclodextrin derivatives with venoruton and rutin by thin layer chromatography

The interaction of rutin and venoruton (troxerutin), with alpha-, beta- and gamma-cyclodextrin (CD), hydroxypropyl-beta-cyclodextrin (HP-beta-CD) and methyl-beta-cyclodextrin (M-beta-CD) was investigated by reversed-phase thin layer chromatography on polyamide plates. A mobile phase consisted of NH(4)OH; NH(4)Cl buffer solution containing various CD concentrations (pH = 9.7, 20 degrees C) was used as mobile phase. The equilibrium constants (K(f)) and the retention factor (R(f)) were determined and used to study the inclusion process. The in fluence of CDs on the solubility of rutin and venoruton was characterized by R(M) values and the increasing hydrophilicity of drugs. The results show that the inclusion capacity of cyclodextrins follows the order HP-beta-CD > M-beta-CD > beta-CD > gamma-CD, and rutin is more easily included by the studied cyclodextrins than venoruton. In addition, the thermodynamic parameters (Delta H, Delta S) for the formation of complexes were obtained from the van't Hoff equation, displaying the enthalpy-entropy compensation effect.     

新型杂多配合物K3H2[GeW8Mo3VO40]·4H2O的合成与晶体结构

用分步酸化、分步加料法合成出新型杂多配合物K3H2[GeW8Mo3VO40]·4H2O,并用X射线单晶衍射法测定其晶体结构,该晶体属单斜晶系,C2空间群,a＝1.894 4(4)nm,b＝3.292 0(7)nm,c＝1.249 4(2)nm,β＝90.25(3)°,V＝7.792(4)nm3,Z＝6.最终偏差因子R＝0.063.RW＝0.067.     

cBN晶体的Raman光谱测量

 用R1000激光共聚焦Raman光谱仪研究了高温高压合成棒中的立方氮化硼（cBN）晶体、原材料六方氮化硼（hBN）和催化剂。Raman光谱测量结果表明：伴随cBN晶体生长的散射峰,出现了两条全新的Raman散射峰（约1.088 cm^-1和约1.368 cm^-1）。该散射峰所对应的物质可能是在高温高压条件下hBN向cBN转变时生成的不完全产物——BN的一种新相。这一结果将有助于进一步讨论cBN的生长机理。     

用遗传算法构造光正交码

提出了一种利用遗传算法构造任意码长、码重、自相关限、互相关限光正交码(GA-OOC)的方法,设计了新的码字矩阵;分析了基于GA-OOC的光CDMA系统的误码率性能。仿真结果表明:该算法具有构造算法简单、易于编程实现的特点;与基于PC,EPC,QC和BIBD-OOC的光码分多址系统相比,GA-OOC的光码分多址系统具有更好的误码率性能。GA-OOC有望用于构造二维光地址码。