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991.
992.
993.
Ruth Kleinewillinghöfer 《Archiv der Mathematik》1980,34(1):469-480
Ohne Zusammenfassung 相似文献
994.
995.
Steven R. Caldwell Gary E. Martin Stanley H. Simonsen Ruth R. Inners M. Robert Willcott 《Journal of heterocyclic chemistry》1981,18(3):479-484
The synthesis of the 3-azaphenoxathiin ring system and its molecular structure are reported. Based on 13C-nmr chemical shift additivities associated with the insertion of an annular nitrogen atom and the observed 13C-nmr shift of Cα, the title compound was predicted to have a dihedral angle θ = 160.2°. The observed dihedral angle from the crystal structure was found to be θ = 167.07° which is in reasonably good agreement with the predicted value. It is proposed that the position of the annular nitrogen atom is solely in-control of the observed dihedral angle. 相似文献
996.
997.
The simulation of the behaviour of two proteins (cytochrom c and alpha-chymotrypsinogen) on two types of stationary phases (monolithic and porous particle-based) was attempted for non-linear (simulation of breakthrough curves) and linear (simulation of elution peaks) cation exchange chromatography. It was found that the combination of a stoichiometric model (steric mass action, SMA) with the lumped pore diffusion (POR) model allows a simulation of high predictive value. Using one set of SMA and transport parameters for a given column morphology and/or protein, breakthrough curves and peaks could be simulated that agreed well with the experimental data while no dependency on either the protein load or the mobile phase composition (salt content) was observed. One of the SMA parameters, namely the characteristic charge needed some slight adjustment (within the error of the experimental determination of this parameter) in order to optimise the fit. 相似文献
998.
ANTIBODIES TO UV IRRADIATED DNA: THE MONITORING OF DNA DAMAGE BY ELISA AND INDIRECT IMMUNOFLUORESCENCE 总被引:6,自引:0,他引:6
Altaf A. Wani Ruth E. Gibson-D'Ambrosio Steven M. D'Ambrosio 《Photochemistry and photobiology》1984,40(4):465-471
Abstract The enzyme-linked immunosorbant assay (ELISA) was modified to (1) characterize antibodies raised in rabbits against UV-irradiated single-stranded DNA (UVssDNA) complexed with methylated BSA and (2) directly detect pyrimidine dimers in irradiated DNA. The antisera specifically bound to UVssDNA, UVpoly(dT) and to a limited extent to UVdsDNA and UVpoly(dC) immobilized on protamine sulfate coated microliter wells. Fifty percent of the maximum antibody binding was observed at a 1-5000 dilution against UVssDNA. Binding to ssDNA and poly(dT) was observed only at much higher concentrations of antibody (1:500 dilution), whereas no binding to double stranded DNA (dsDNA) was observed. The extent of binding of the antibody was dependent on the dose of UV radiation to DNA, as well as, to the concentration of antigen immobilized on the plate. Specific binding to DNA irradiated with 5.0 J/m2 was detected with as little as 10 ng of DNA. The sensitivity was further extended to less than 1 J/m2 by using higher concentrations (100 ng) of UVssDNA. The ability of various irradiated molecules, DNA, homopolymers and linkers to act as inhibitors of antibody binding establish that the antigenic determinants are mainly thymine homodimers with lower affinity for cytosine dimers. Potential usefulness of the antibodies to directly quantitate pyrimidine dimers in cells exposed to UV radiation was determined by indirect immunofluorescence. Flow cytometric analysis of immunostained human lymphocytes irradiated with 254 nm radiation indicated that greater than 50% of the population had significantly higher fluorescent intensity than unirradiated control cells. 相似文献
999.
Ruth E. Fake 《Tetrahedron letters》2004,45(48):8925-8926
A rapid method for the qualitative detection of hydroxyl groups on solid-phase has been developed. The method employs N-methylisatoic anhydride to derivatise resin-bound substrates possessing free hydroxyl functionality. The resultant fluorescent ester can be detected by visualisation under a standard laboratory UV lamp at 365 nm excitation. 相似文献
1000.
Christina L.L Chai Alison J EdwardsBronwyn A Wilkes Ruth C.J Woodgate 《Tetrahedron》2003,59(44):8731-8739
The reactivities and selectivities of 1,3-dipolar cycloaddition reactions of ylidene piperazine-2,5-diones with mesitonitrile oxide are reported. The stereoselectivities of reactions with chiral ylidene piperazine-2,5-diones can be directed by judicious choice of substituents on the N- and/or C-substituents of the piperazinedione ring. 相似文献