排序方式: 共有38条查询结果,搜索用时 31 毫秒
11.
Bakalova R Zhelev Z Ohba H Baba Y 《Journal of the American Chemical Society》2005,127(32):11328-11335
In the present study, we describe the design and fabrication of quantum dot-conjugated hybridization probes and their application to the development of a comparatively simple and rapid procedure for the selection of highly effective small-interfering RNA (siRNA) sequences for RNA interference (RNAi) in mammalian cells, for example, siRNAs with high accessibility and affinity to the respective mRNA target. A single-stranded siRNA was conjugated with a quantum dot and used as a hybridization probe. The target mRNA was amplified in the presence of Cy5-labeled nucleotides, and Cy5-mRNA served as a hybridization sample. The formation of siRNA/mRNA duplexes during a comparatively short hybridization time (1 h) was used as a criterion for the selection of highly effective, target-specific siRNA sequences. The accessibility and affinity of the siRNA sequence for the target mRNA site were determined by fluorescence resonance energy transfer (FRET) between a quantum dot (donor) and a fluorescent dye molecule (Cy5, acceptor) localized at an appropriate distance from each other when hybridization occurred. The FRET signal was observed only when there was high accessibility between an antisense siRNA and a sense mRNA and did not appear in the case of mismatch siRNAs. Moreover, the amplitude of the FRET signal significantly correlated with the specific effect of siRNA on the expression of the target mRNA and protein, determined in native cells by RT-PCR and immunoblot analysis, respectively. 相似文献
12.
Single quantum dot-micelles coated with silica shell as potentially non-cytotoxic fluorescent cell tracers 总被引:1,自引:0,他引:1
The present study describes a stabilization of single quantum dot (QD) micelles by hydrophobic silica precursors and an extension of the silica layer to form a silica shell around the micelle. The obtained product consists of up to 92% of single nanocrystals (CdSe, CdSe/ZnS, or CdSe/ZnSe/ZnS quantum dots) in the silica micelles, coated with silica shell. The thickness of silica shell could vary, starting from 3 to 4 nm. Increasing the shell thickness increases the photoluminescent characteristics of QDs in aqueous solution. The silica-shelled single CdSe/ZnS QD micelles possess a high quantum yield in aqueous solution, a controlled small size, sharp photoluminescence spectra (fwhm approximately 30 nm), an absence of aggregation, and a high transparency. The presence of a hydrophobic layer between the QD and silica shell ensures an incorporation of other hydrophobic molecules (with interesting properties) in the close proximity of nanocrystal. Thus, it is possible to combine the characteristics of hybrid material with the priority of small size. The nanoparticles are amino functionalized and ready for conjugation. A comparatively good biocompatibility is demonstrated. The nanoparticles show ability for intracellular delivery and are noncytotoxic during long-term incubation with viable cells in the absence of light exposure, which makes them appropriate for cell tracing and drug delivery. 相似文献
13.
R. Kotsilkova V. Petkova Y. Pelovski 《Journal of Thermal Analysis and Calorimetry》2001,64(2):591-598
The thermal behavior of epoxy-smectite nanocomposites (hybrids) is examined by non-isothermal thermogravimetry (TG, DTG and
DTA) in air atmosphere. It has been shown that the thermal stability of hybrids is much greater than that of epoxy resin and
strongly depends on both the smectite loading and the type of the gallery cations of organically modified smectites. The kinetics
of degradation of nanocomposites is significantly influenced by the presence of smectites and proceeds in three stages. Stage
I is attributed to the effect of quaternized ammonium ion exchanged smectite, as stages II and III are associated with the
decomposition of the bulk epoxy resin. Because of the interfacial interactions and thesilicate-polymer multilayered nanoscale
organization, the nanocomposites act as excellent heat insulator and mass transport barrier, which shift the thermal decomposition
peaks towards much higher temperatures.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
14.
Quantum dot-based western blot technology for ultrasensitive detection of tracer proteins 总被引:6,自引:0,他引:6
The present study describes two quantum dot-based Western blotting protocols for ultrasensitive detection of "tracer" proteins in cell lysates. 相似文献
15.
A nonlinear problem for penetrant diffusion with relaxation in polymers is considered. A numerical approach to solving this type of problems is developed. The proposed numerical scheme based on a finite element domain approximation and a time difference method can be used for numerical simulation of the considered penetrant diffusion in 2-D and 3-D domains. A numerical procedure and a corresponding computer code are created and tested for some examples in 1-D and 2-D domains. 相似文献
16.
Design and synthesis of fluorescent shell functionalized polymer micelles for biomedical application
Selen R. Ismail Rayna G. Bryaskova Nikolai I. Georgiev Nikoleta D. Philipova Ventsislav V. Bakov Veselina P. Uzunova Rumiana D. Tzoneva Vladimir B. Bojinov 《先进技术聚合物》2020,31(6):1365-1376
pH‐sensitive polymers can be defined as polyelectrolytes that include in their structure weak acidic or basic groups that either accept or release protons in response to a change in the environmental pH. This work summarizes the design, synthesis, and potential applications of pH‐responsive fluorescent copolymers in the biomedical field. This was achieved using atom transfer radical polymerization (ATRP) of tert‐butyl acrylate using a CuBr/N,N,N′,N″N″‐pentamethyldiethylenetriamine catalyst system in conjunction with an alkyl bromide as the initiator. Well‐defined macroinitiators based on poly(tert‐butyl acrylate) with narrow molecular weight distributions were obtained by the addition of an appropriate solvent system in order to create a homogeneous catalytic system. The addition of n‐butyl acrylate as a second building block in order to create well‐defined poly(tert‐butyl acrylate)‐b‐poly(n‐butyl acrylate) block copolymers (PtBA‐b‐PnBA) followed by chemical modification of the block copolymers and functionalization with an appropriate fluorescent compound are the basis for the preparation of well‐defined fluorescent pH‐sensitive micelles. Thus, prepared water soluble nanosized pH‐sensitive micelles consisting of hydrophobic poly(n‐butyl acrylate) core and hydrophilic polyacrylic acid shell decorated with an appropriate fluorescent compound determined their potential applications of these systems in the field of biomedicine as biosensors, controlled drug delivery systems, and so on. In this respect, the cell viability and internalization of the polymer micelles were studied. 相似文献
17.
R. Kotsilkova D. Fragiadakis P. Pissis 《Journal of Polymer Science.Polymer Physics》2005,43(5):522-533
The reinforcing effect of carbon nanoparticles in an epoxy resin has been estimated with different approaches based on rheology, molecular dynamics (evaluated by differential scanning calorimetry, dielectric relaxation spectroscopy, and thermally stimulated depolarization current), and dynamic mechanical analysis. Carbon particles aggregate as the volume increases and form a fractal structure in the matrix polymer. The dispersion microstructure has been characterized by its viscoelastic properties and relaxation time spectrum. The scaling of the storage modulus and yield stress with the volume fraction of carbon shows two distinct exponents and has thus been used to determine the critical carbon volume fraction of the network formation (Φ*) for the carbon/epoxy dispersions. At nanofiller concentrations greater than Φ*, the overall mobility of the polymer chains is restricted in both dispersions and solid nanocomposites. Therefore, (1) the relaxation spectrum of the dispersions is strongly shifted toward longer times, (2) the glass‐transition temperature is increased and (3) the relaxation strength of both the secondary (β) and primary (α) relaxations increases in the nanocomposites, with respect to the pure polymer matrix. The dispersion microstructure, consisting of fractal flocs and formed above Φ*, is proposed to play the main role in the reinforcement of nanocomposites. Moreover, the network structure and the interface polymer layer (bond layer), surrounding nanoparticles, increases the relaxation strength and slows the cooperative α relaxation, and this results in an improvement of the mechanical properties. © 2005 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 43: 522–533, 2005 相似文献
18.
The present study describes a simultaneous and highly reproducible large-scale synthesis of six (and more) colors of size-homogeneous and highly luminescent CdSe quantum dots in a single reaction, controlled by a slow-increasing temperature gradient. The described protocol allows a precise control and a synchronized isolation of aliquots of CdSe nanocrystals with defined sizes, avoiding disturbance of the growth of nanocrystals (existing in the reaction mixture) to the isolation of the next aliquot. The obtained quantum dot fractions are of high quality (in 95% size-homogeneous) and have sharp photoluminescence spectra (fwhm approximately 30 nm), quantum yields of 45-70% (in organic solvent), and a lack of aggregation in organic solvents. The method is environmentally friendly as it ensures almost complete utilization of the precursors and productive yield approximately 95%. 相似文献
19.
We describe a facile method to simultaneously measure the bending rigidity and capacitance of biomimetic lipid bilayers. Our approach utilizes the ellipsoidal deformation of quasi-spherical giant unilamellar vesicles induced by a uniform AC electric field. Vesicle shape depends on the electric field frequency and amplitude. Membrane bending rigidity can be obtained from the variation of the vesicle elongation on either field amplitude at fixed frequency or frequency at fixed field amplitude. Membrane capacitance is determined from the frequency at which the vesicle shape changes from prolate to oblate ellipsoid as the frequency is increased at a given field amplitude. 相似文献
20.
Binding of chitosan to phospholipid vesicles studied with isothermal titration calorimetry 总被引:1,自引:0,他引:1
We thermodynamically characterize the interaction of chitosan with small liposomes and the binding and organization of the polysaccharide on the membrane of the vesicles. By means of isothermal titration calorimetry (ITC), we obtain the enthalpy variations arising from binding of the positively ionized chitosan to neutral and negatively charged liposomes. The strong electrostatic interaction of the polysaccharide with the negative charges at the membrane gives rise to highly exothermic signal until charge compensation is reached. The equilibrium constant, the interaction stoichiometry, and the molar enthalpy of binding chitosan monomers to phospholipids from the external leaflet of the vesicle membrane are obtained from the isotherm curve fitting assuming independent binding sites. The strong exothermic signal indicates that the electrostatically driven binding of chitosan to the membrane is energetically favored, leading to further stabilization of the vesicle suspension. The higher the net negative charge of the vesicles, the more pronounced the adsorption of chitosan is, leading to weaker chain organization of the adsorbed chitosan at the membrane. At the point of charge saturation, vesicle aggregation takes place and we show that this behavior does not always lead to charge reversal at the membrane. Models for the binding behavior and structural organization of chitosan are proposed based on the experimental results from ITC, ζ-potential, and dynamic light scattering. 相似文献