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Elvira Welzig Harald Pichler Rudolf Krska Dietmar Knopp Reinhard Niessner 《International journal of environmental analytical chemistry》2013,93(3-4):279-288
Abstract The development of an indirect competitive enzyme immunoassay for the sulfonylurea herbicide metsulfuron-methyl (MSM) is described. In contrast to traditional antibody generation in mammals, this extremely sensitive method is based on chicken egg yolk antibodies (IgY). They were raised in laying hens using an MSM-derivative-BSA hapten as immunogen. With a 1:10000 dilution of the antibody solution and a coating antigen (MSM-derivative-KLH) concentration of 10 μg L?1 the IC50 value achieved for the target analyte was 0.4 μg L?1. The least detectable dose was established at 13 ng L?1. Cross-reactivity was tested with 5 structurally related compounds, where only sulfometuron showed a significant binding. The ELISA was tested with spiked tap and surface water samples. This paper, for the first time, demonstrates the production of high-affinity IgY antibodies for a herbicide compound. 相似文献
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Abstract Biphasic blends (mixtures) of liquid crystal ionomers (prepared from ferrocene containing liquid crystal polymers by a redox reaction) and partially sulfonated polystyrene combine the constituent properties in a way, which is dependent on the measuring method. Polarizing microscopy shows homogenous textures similar to the pure liquid crystal polymer. Mechanical measurements show however the elastomeric behaviour of sulfonated polystyrene. The phase separated structure of these blends is proved by differential scanning calorimetry measurements. 相似文献
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Aleksandr I. Chumakov Ilya Sergeev Jean‐Philippe Celse Rudolf Rüffer Marc Lesourd Lin Zhang Manuel Sánchez del Río 《Journal of synchrotron radiation》2014,21(2):315-324
The performance of a cryogenically cooled double‐crystal silicon monochromator was studied under high‐heat‐load conditions with total absorbed powers and power densities ranging from 8 to 780 W and from 8 to 240 W mm?2, respectively. When the temperature of the first crystal is maintained close to the temperature of zero thermal expansion of silicon, the monochromator shows nearly ideal performance with a thermal slope error of 0.6 µrad. By tuning the size of the first slit, the regime of the ideal performance can be maintained over a wide range of heat loads, i.e. from power densities of 110 W mm?2 (at total absorbed power of 510 W) to 240 W mm?2 (at total absorbed power of 240 W). 相似文献
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