全文获取类型
收费全文 | 200篇 |
免费 | 6篇 |
国内免费 | 2篇 |
专业分类
化学 | 167篇 |
力学 | 6篇 |
数学 | 9篇 |
物理学 | 26篇 |
出版年
2023年 | 1篇 |
2022年 | 3篇 |
2021年 | 4篇 |
2020年 | 13篇 |
2019年 | 16篇 |
2018年 | 10篇 |
2017年 | 9篇 |
2016年 | 16篇 |
2015年 | 5篇 |
2014年 | 13篇 |
2013年 | 22篇 |
2012年 | 21篇 |
2011年 | 15篇 |
2010年 | 10篇 |
2009年 | 10篇 |
2008年 | 11篇 |
2007年 | 3篇 |
2006年 | 2篇 |
2005年 | 5篇 |
2004年 | 3篇 |
2003年 | 3篇 |
2002年 | 2篇 |
2001年 | 1篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1993年 | 1篇 |
1986年 | 1篇 |
1984年 | 5篇 |
1978年 | 1篇 |
排序方式: 共有208条查询结果,搜索用时 15 毫秒
11.
Highly sensitive FRET-based fluorescence immunoassay for aflatoxin B1 using cadmium telluride quantum dots 总被引:1,自引:0,他引:1
Roya Zekavati Shahabeddin Safi Seyed Jamal Hashemi Tavoos Rahmani-Cherati Meisam Tabatabaei Afshin Mohsenifar Mansour Bayat 《Mikrochimica acta》2013,180(13-14):1217-1223
We report on a competitive immunoassay for the determination of aflatoxin B1 using fluorescence resonance energy transfer (FRET) from anti-aflatoxin B1 antibody (immobilized on the shell of CdTe quantum dots) to Rhodamine 123 (Rho 123-labeled aflatoxin B1 bound to albumin). The highly specific immunoreaction between the antibody against aflatoxin B1 on the QDs and the labeled-aflatoxin B1 brings the Rho 123 fluorophore (acting as the acceptor) and the QDs (acting as the donor) in close spatial proximity and causes FRET to occur upon photoexcitation of the QDs. In the absence of unlabeled aflatoxin B1, the antigen-antibody complex is stable, and strong emission resulting from the FRET from QDs to labeled aflatoxin B1 is observed. In the presence of aflatoxin B1, it will compete with the labeled aflatoxin B1-albumin complex for binding to the antibody-QDs conjugate so that FRET will be increasingly suppressed. The reduction in the fluorescence intensity of the acceptor correlates well with the concentration of aflatoxin B1. The feasibility of the method was established by the detection of aflatoxin B1 in spiked human serum. There is a linear relationship between the increased fluorescence intensity of Rho 123 with increasing concentration of aflatoxin B1 in spike human serum, over the range of 0.1–0.6 μmol·mL?1. The limit of detection is 2?×?10?11 M. This homogeneous competitive detection scheme is simple, rapid and efficient, and does not require excessive washing and separation steps. Figure
A nanobiosensor has been fabricated based on a competitive immunoassay for the determination of aflatoxin B1 using fluorescence resonance energy transfer (FRET). In the presence of aflatoxin B1, it will compete with the labeled aflatoxin B1-albumin complex for binding to the antibody-QDs conjugate so that FRET will be increasingly suppressed. 相似文献
12.
An efficient synthesis of new pharmaceutically relevant dioxopyrrolidines, spirobenzo thiazine-2,3′-chromans, and benzothiazepines via isocyanide-based multicomponent condensation reactions at room temperature is reported. This synthesis serves as a nice addition to group-assistant-purification (GAP) chemistry in which purification via chromatography and recrystallization can be avoided, and the pure products are obtained simply by washing the crude products with 95% ethanol. 相似文献
13.
Bahareh Heidari Majid M. Heravi Mohammad Reza Nabid Roya Sedghi Seyyed Emad Hooshmand 《应用有机金属化学》2019,33(1)
A novel nanocatalyst was designed and prepared. Initially, the surface of magnetic graphene oxide (M‐GO) was modified using thionyl chloride, tris(hydroxymethyl)aminomethane and acryloyl chloride as linkers which provide reactive C═C bonds for the polymerization of vinylic monomers. Separately, β‐cyclodextrin (β‐CD) was treated with acryloyl chloride to provide a modified β‐CD. Then, in the presence methylenebisacrylamide as a cross‐linker, monomers of modified β‐CD and acrylamide were polymerized on the surface of the pre‐prepared M‐GO. Finally, palladium acetate and sodium borohydride were added to this composite to afford supported palladium nanoparticles. This fabricated nanocomposite was fully characterized using various techniques. The efficiency of this easily separable and reusable heterogeneous catalyst was successfully examined in Suzuki–Miyaura cross‐coupling reactions of aryl halides and boronic acid as well as in modified Suzuki–Miyaura cross‐coupling reactions of N‐acylsuccinimides and boronic acid in green media. The results showed that the nanocatalyst was efficient in coupling reactions for direct formation of the corresponding biphenyl as well as benzophenone derivatives in green media based on bio‐based solvents. In addition, the nanocatalyst was easily separable, using an external magnet, and could be reused several times without significant loss of activity under the optimum reaction conditions. 相似文献
14.
Seyyed Javad Sabounchei Khadijeh Badpa Mehdi Bayat Roya Karamian Fatemeh Ghasemlou Robert W. Gable Parviz Gohari Derakhshandeh Kristof Van Hecke 《应用有机金属化学》2019,33(3)
The reaction of α‐keto‐stabilized diphosphine ylides [Ph2P(CH2)nPPh2═C(H)C(O)C6H4‐p‐CN] (n = 1 (Y1); n = 2 (Y2)) with dibromo(1,5‐cyclooctadiene) palladium(II)/platinum(II) complexes, [Pd/PtBr2(cod)], in equimolar ratio gave the new cyclometalated Pd(II) and Pt(II) complexes [Br2Pd(κ2‐Y1)] ( 1 ), [Br2Pt(κ2‐Y1)] ( 2 ), [Br2Pd(κ2‐Y2)] ( 3 ) and [Br2Pt(κ2‐Y2)] ( 4 ). These compounds were screened in a search for novel antibacterial agents and characterized successfully using Fourier transfer infrared and NMR (1H, 13C and 31P) spectroscopic methods. Also, the structures of complexes 1 and 2 were characterized using X‐ray crystallography. The results showed that the P,C‐chelated complexes 1 and 2 have structures consisting of five‐membered rings, while 3 and 4 have six‐membered rings, formed by coordination of the ligand through the phosphine group and the ylidic carbon atom to the metal centre. Also, a theoretical study of the structures of complexes 1 – 4 was conducted at the BP86/def2‐SVP level of theory. The nature of metal–ligand bonds in the complexes was investigated using energy decomposition analyses (EDA) and extended transition state combined with natural orbitals for chemical valence analyses. The results of EDA confirmed that the main portions of ΔEint, about 57–58%, in the complexes are allocated to ΔEelstat. 相似文献
15.
16.
Reza Sepahvand Mohsen Adeli Bandar Astinchap Roya Kabiri 《Journal of nanoparticle research》2008,10(8):1309-1318
Metal-carbon nanotube-graft-polymer (MCNT-g-P) nanocomposites were synthesized and characterized successfully. In this work, multiwall carbon nanotubes (MWCNT) were
opened using HNO3/H2SO4 mixture and filled by metal nanoparticles such as silver nanoparticles through wet chemistry method. Then MWCNT containing
metal nanoparticles were used as macroinitiator for ring opening polymerization of ε-caprolactone and MCNT-g-P nanocomposites were obtained. Length of grafted polymer arms onto the MWCNT was controlled using MWCNT/ε-caprolactone ratio.
Structure and properties of nanocomposites were evaluated by TEM, DSC, TGA, and spectroscopy methods. 相似文献
17.
M.R. Bayati Roya Molaei F. Golestani-Fard 《Colloids and surfaces. A, Physicochemical and engineering aspects》2011,373(1-3):51-60
This study sheds light on the effect of vanadium doping on hydrophilicity properties of micro arc oxidized TiO2 porous layers. Pure and V-doped titania layers, with a pore size of 50–400 nm, were grown by micro arc oxidation method. Morphology and topography of the layers were studied by SEM and AFM techniques where formation of a porous structure with a rough surface was confirmed. Moreover, phase structure and chemical composition of the samples were investigated employing XRD and XPS techniques. The pure TiO2 layers consisted of anatase and rutile phase. Vanadia phase was also detected in V-doped layers. It was also revealed that V2O5 not only dispersed in the TiO2 matrix, but also doped into the crystalline lattice. Optical properties and band gap energy of the synthesized layers were evaluated by a UV-Vis spectrophotometer. Our results showed that the band gap energy decreased when vanadium was introduced into the titania lattice. Finally, hydrophilicity of the pure and the doped layers was studied under ultraviolet and visible illuminations by measuring the water contact angle on their surface. The V-doped layers, especially those which were grown under intermediate voltages, revealed an enhanced hydrophilicity when compared to the pure TiO2 layers. 相似文献
18.
A method for sensitive and selective determination of silver based on the catalytic effect of silver(I) ion on the oxidation of Janus Green by peroxodisulfate is described. o-Phenanthroline is used as an activator. The rate of the decrease in absorbance of Janus Green (at 615 nm) is proportional to the concentration of silver in the range of 0.3-4.0 ng mL(-1) and 4.0-500.0 ng mL(-1). The theoretical limit of detection was 0.25 ng mL(-1). The method is free from most interferences. The method was applied to the determination of silver in plants (the uptake of silver by plants), in photographic solutions, lake water and several synthetic samples. 相似文献
19.
Rahman Bikas Massomeh Ghorbanloo Roya Sasani Ingo Pantenburg Gerd Meyer 《Journal of Coordination Chemistry》2017,70(5):819-830
The reaction between tridentate NNO donor hydrazone ligands, (E)-2-cyano-N′-(phenyl(pyridin-2-yl)methylene)acetohydrazide (HL1) and (E)-2-cyano-N′-(1-(pyridin-2-yl)ethylidene)acetohydrazide (HL2), with MnCl2·4H2O in methanol resulted in [Mn(HL1)Cl2(CH3OH)] (1) and [Mn(HL2)Cl2(CH3OH)] (2). Molecular structures of the complexes were determined by single-crystal X-ray diffraction. All of the investigated compounds were further characterized by elemental analysis, FT-IR, TGA, and UV–Vis spectroscopy. These complexes were used as catalysts for olefin oxidation in the presence of tert-butylhydroperoxide (TBHP) as an oxidant. Under similar experimental conditions with equal manganese loading, the presence of [Mn(HL2)Cl2(CH3OH)] (2) resulted in higher conversion than [Mn(HL1)Cl2(CH3OH)] (1). 相似文献
20.
Roya Akbarzadeh 《Journal of organometallic chemistry》2010,695(21):2320-3424
An efficient and simple synthesis of ferrocenyl bis-amides by the Ugi four-component reaction of ferrocenecarboxaldehyde, carboxylic acids, isocyanides and amines in methanol at room temperature is reported. 相似文献